Multifunctional Cytokinesis Genes in Schizosaccharomyces pombe
The proper division of cells is essential for the production of viable daughter cells. In plants and fungi, the dividing cell produces a cross-wall or septum that bisects the cytoplasm. For separation of the daughter cells, the septum has to be cleaved. To study the regulation of this process, we isolated mutants defective in septum cleavage. The mutants showed highly pleiotropic phenotypes and defined 17 novel genes. The deduced amino acid sequences of the products of the cloned genes exhibited homologies to various transcription regulators of other organisms. The homologies and the pleiotropic effects of the mutations on sexual development, stress response, mitotic stability, septum initiation and septum placement indicated that these genes affect cell separation indirectly, through multifunctional regulatory modules.
Keywordscytokinesis yeast Schizosaccharomyces transcription sterility
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- 10.Grallert, A., Sipiczki, M. (1999) Identification of novel sep genes of Schizosaccharomyces pombe. Cancer and Cell Cycle. ISREC Conference. Lausanne (Switzerland). Abstract Book PA-87.Google Scholar
- 18.Marks, J., Hyams, J. S. (1985) Localization of F-actin through the cell division cycle of S pombe. Eur J. Cell Biol 39, 27–32.Google Scholar
- 20.Miklös, I., Sipiczki, M. (1999) Analysis of a Schizosaccharomyces pombe cytokinesis mutant strain defective in the spl1 gene. Curr. Gen. 35, 439.Google Scholar
- 28.Sipiczki, M., Bozsik, A. (2000) The use of morphomutants to investigate septum formation and cell separation in Schizosaccharomyces pombe. Arch. Microbiol. (in press).Google Scholar
- 32.Szilágyi, Z., Grallert, A., Zilahi, E., Sipiczki, M. (2000) The Schizosaccharomyces pombe sep10 gene encodes a conservative protein that presumably plays a role in transcription of a subset of genes. First Joint Meeting of the Slovenian Society for Microbiology and the Hungarian Society for Microbiology. Keszthely, Hungary. Programme and Abstracts p. 67.Google Scholar
- 33.Zilahi, E., Miklós, I., Sipiczki, M. (2000) The Schizosaccharomyces pombe sep15+ gene encodes a protein homologous to the Med8 subunit of the Saccharomyces cerevisiae transcriptional mediator complex. Curr Genet. (in press).Google Scholar
- 34.Zilahi, E., Salimova, E., Simanis, V., Sipiczki, M. (2000) The S pombe sep1 gene encodes a nuclear protein that influences the expression of the cdc15 gene. FEBS Letters (in press).Google Scholar
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