Salt Tolerant Wheat Landraces and Gly II Transformed Lines Show Distinct Biochemical Mechanisms of Stress Tolerance
The present investigation was carried out to study the distinct salt tolerance mechanism in two sets of material, Gly II transgenics and Kharchia landraces. The Gly II transgenics were developed for glyoxalase II (osglyII) gene (GenBank accession no. AY054407) from Oryza sativa through Agrobacterium mediated method in the background of wheat cultivar PBW 621. Kharchia 65 is a salt tolerant landrace derivative developed from Kharchia local which is native to saline soils of Rajasthan. The six wheat genotypes, viz. Kharchia local, Kharchia 65, PBW 621, G-2-2, G-3-4 and G-1-13 were evaluated for growth parameters, antioxidant enzymes and contents of glutathione, ascorbic acid, malondialdehyde (MDA), H2O2, sugars, chlorophyll, carotenoid, electrolyte leakage (EL) and Na+, K+ under control and two salt treatments (150 mM and 250 mM NaCl). The activities of antioxidant enzymes, glutathione, sugar content increased in both GlyII and Kharchia genotypes as compared to PBW 621. The GlyII activity increased (77–84%) in GlyII genotypes alongwith content of reduced glutathione (GSH) to maintain redox homeostasis. Apparently, GlyII and Kharchia genotypes exhibited minimum oxidative stress due to low content of MDA, H2O2, diminished EL and thereby causing less growth reduction and maintaining high chlorophyll and carotenoid level as compared to PBW 621. In addition, Gly II transgenic material and Kharchia lines showed less Na+ accumulation, greater seedling biomass and sugar content due to its salt tolerance mechanism. We infer that GlyII activity enhances GSH which play significant role in detoxifying ROS to establish stress homeostasis. The route for generation of GSH is via ascorbate-glutathione pathway mediated by glutathione reductase. Hence, GlyII transgenics and Kharchia genotypes can diminish salt stress following above mechanism.
Keywordsantioxidants GlyII transgenics glyoxalase pathway wheat salt treatments
glutathione s transferase
reactive oxygen species
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- FAO. 2016. FAO Soils Portal. Available at: https://doi.org/www.fao.org/soils-portal/soilmanagement/management-of-some-problem-soils/salt-affected soils/more information-on-salt-affected-soils/en.
- Gupta, B.K., Sahoo, K.K., Ghosh, A., Tripathi, A.K., Khalid, A., Das, P., Singh, A.K., Pareek, A., Sopory, S.K., Singla-Pareek, S.L. 2018. Manipulation of glyoxalase pathway confers tolerance to multiple stresses in rice Plant Cell and Environ. 41:1186–1200.Google Scholar
- Gurmani, A.R., Khan, S.U., Mabood, F., Ahmed, Z., Butt, S.J., Din, J., Mujeeb-Kazi, A., Smith, D. 2014. Screening and selection of synthetic hexaploid wheat germplasm for salinity tolerance based on physiological and biochemical characters. Int. J. Agric. Biol. 16:681–690.Google Scholar
- Hoagland, D.R., Arnon, D. 1950. The water culture method for growing plants without soil. Circular 347, California Agricultural Experiment Station, University of California-Berkeley, Berkeley Ca, USA.Google Scholar
- Hussain, M.I., Shah, S., Hussain, S., Iqbal, K. 2002. Growth, yield and quality response of three wheat (Triticum aestivum L.) varieties to different levels of N, P and K. Int. J. Agric. Biol. 4(3):362–364.Google Scholar
- Joshi, Y.C., Ali, Q., Bal, A.R., Rana, R.S. 1980. Sodium/potassium index of wheat seedlings in relation to sodicity tolerance, International symposium on salt affected soils, Feb 18–21. CSSRI. Karnal pp. 451–460.Google Scholar
- Kaur, R. 2014. Genetic transformation of bread wheat (Triticum aestivum L.) by ‘particle gun and Agrobacterium-mediated approaches. Ph.D. dissertatioin. Punjab Agricultural University, Ludhiana, India.Google Scholar
- Kaya, C., Ashraf, M., Dikilitas, M., Tuna, A.L. 2013. Alleviation of salt stress induced adverse effects on maize plants by exogenous application of indoleacetic acid (IAA) and inorganic nutrients – a field trial. Aust. J. Crop Sci. 7:249–254.Google Scholar
- Kumar, M., Hasan, M., Arora, A., Gaikwad, K., Kumar, S., Rai, R.D. 2015. Sodium chloride-induced spatial and temporal manifestation in membrane stability index and protein profiles of contrasting wheat (Triticum aestivum L.) genotypes under salt stress. Ind. J. Plant Physiol. 20:271–275.CrossRefGoogle Scholar
- Passaia, G., Spagnolo, F.L., Caverzan, A., Jardim-Messeder, D., Christoff, A.P., Gaeta, M.L., de Araujo Mariath, J.E., Margis, R., Margis-Pinheiro, M. 2013. The mitochondrial glutathione peroxidase GPX3 is essential for H2O2 homeostasis and root and shoot development in rice. Plant Sci. 208:93–101.PubMedCrossRefPubMedCentralGoogle Scholar
- Rahaie, M., Xue, G.P., Schenk, P.M. 2013. The role of transcription factors in wheat under different abiotic stresses. In: Vahdati, K. and Leslie, C. (eds) Abiotic Stress. Plant Responses and Applications in Agriculture. In Tech, Rijeka, Croatia, pp. 367–385.Google Scholar
- Singh, V., Singh, A.P., Bhadoria, J., Giri, J., Singh, J., Vineeth, T.V., Sharma, P.C. 2018. Differential expression of salt-responsive genes to salinity stress in salt tolerant and salt-sensitive rice (Oryza sativa L.) at seedling stage. Protoplasma 255:1665–1681.Google Scholar
- Valentine, W.N., Paglia, D.E. 1987. Studies on the quantitative and qualities characterization of glutathione peroxidase. J. Laboratory Clin. Med. 70:158–165.Google Scholar
- Xie, J., Dai, Y., Mu, H., De, Y., Chen, H., Wu, Z., Ren, W. 2016. Physiological and biochemical responses to NACl salinity stress in three Roegneria (Poaceae) species. Pakistan J. Bot. 48(6):2215–2222.Google Scholar