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Cereal Research Communications

, Volume 46, Issue 2, pp 333–343 | Cite as

Comparison of Endosperm Amyloplast Development and Degradation in Waxy and Non-waxy Wheat

  • H. Yu
  • Y. Yang
  • X. Y. Chen
  • G. X. Lin
  • J. Y. Sheng
  • J. Y. Nie
  • Q. J. Wang
  • E. J. Zhang
  • X. R. Yu
  • Z. Wang
  • F. XiongEmail author
Article

Abstract

The waxy wheat shows special starch quality due to high amylopectin content. However, little information is available concerning the development and degradation of amyloplast from waxy wheat endosperm. To address this problem, waxy wheat variety, Yangnuo 1, and a non-waxy wheat variety, Yangmai 13, were chosen to investigate the development and degradation of endosperm amyloplast during wheat caryopsis development and germination stage respectively using histochemical staining and light microscopy. Changes of morphology, the soluble sugar and total starch content were indistinguishable in the process of caryopsis development of two wheat varieties. The developing endosperm of non-waxy was stained blue-black by I2-KI while the endosperm of waxy wheat was stained reddish-brown, but the pericarp of waxy and non-waxy wheat was stained blue-black. In contrast to nonwaxy wheat, endosperm amyloplast of waxy wheat had better development status and higher proportion of small amyloplast. During seed germination many small dissolution pores appeared on the surface of endosperm amyloplast and the pores became bigger and deeper until amyloplast disintegrated. The rate of degradation of waxy wheat endosperm amyloplast was faster than non-waxy wheat. Our results may also be helpful to the use of waxy starch in food and nonfood industry.

Keywords

waxy wheat amyloplast development degradation 

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References

  1. Baik, B.K., Lee, M.R. 2003. Effects of starch amylose content of wheat on textural properties of white salted noodles. Cereal Chem. 80:304–309.CrossRefGoogle Scholar
  2. Blazek, J., Copeland, L. 2010. Amylolysis of wheat starches. II. Degradation patterns of native starch granules with varying functional properties. J. Cereal Sci. 52:295–302.Google Scholar
  3. Chao, S., Sharp, P.J., Worland, A.J., Warham, E.J., Koebner, R.M.D., Gale, M.D. 1989. RFLP-based genetic maps of wheat homoeologous group7 chromosomes. Theor. Appl. Genet. 78:495–504.CrossRefGoogle Scholar
  4. Colonna, P., Buleon, A., Lemarie, F. 1988. Action of Bacillus subtilis α-amylase on native wheat starch. Biotechnol Bioeng. 31:895–904.CrossRefGoogle Scholar
  5. Dodson, J.R., Li, X., Zhou, X., Zhao, K., Sun, N., Atahan, P. 2013. Origin and spread of wheat in China. Quaternary Sci. Rev. 72:108–111.CrossRefGoogle Scholar
  6. Domínguez, F., Cejudo, F.J. 2014. Programmed cell death (PCD): an essential process of cereal seed development and germination. Front Plant Sci. 5:1–11.Google Scholar
  7. He, J.F., Goyal, R., Laroche, A., Zhao, M.L., Lu, Z.X. 2012. Water stress during grain development affects starch synthesis, composition and physicochemical properties in triticale. J. Cereal Sci. 56:552–560.CrossRefGoogle Scholar
  8. Hung, P.V., Maeda, T., Morita, N. 2006. Waxy and high-amylose wheat starches and fours-characteristics, functionality and application. Trends Food Sci. Tech. 17:448–456.CrossRefGoogle Scholar
  9. Li, W.H., Shan, Y.L., Xiao, X.L., Luo, Q.G., Zheng, J.M., Ouyang, S.H., Zhang, G.Q. 2013. Physicochemical properties of A- and B- starch granules isolated from hard red and soft red winter wheat. J. Agr. Food Chem. 61:6477–6484.CrossRefGoogle Scholar
  10. Ma, H.B., Zhang, X., Wang, C.G., Gao, D.R., Zhang, B.Q., Lv, G.F., Wu, R.L., Cheng, X.M., Wang, X., Cheng, S.H., Bie, T.D. 2013. Effect of wx genes on amylose content, physicochemical properties of wheat starch, and the suitability of waxy genotype for producing Chinese crisp sticks. J. Cereal Sci. 58:140–147.CrossRefGoogle Scholar
  11. Macdonald, F.D., Preiss, J. 1985. Partial purification and characterization of granule-bound starch syntheses from normal and waxy maize. Plant Physiol. 78:849–852.CrossRefGoogle Scholar
  12. Mira, I., Persson, K., Kurtis, V.V. 2007. On the effect of surface active agents and their structure on the temperature-induced changes of normal and waxy wheat starch in aqueous suspension. Part I. Pasting and calorimetric studies. Carbohyd. Polym. 68:665–678.Google Scholar
  13. Morell, M.K, Rahman, S.L., Abrahams, S., Appels, R. 1995. The biochemistry and molecular biology of starch synthesis in cereals. Aust. J. Plant Physiol. 22:647–660.Google Scholar
  14. Morrison, W.R., Milligan, T.P., Azudin, M.N. 1984. A relationship between the amylose and lipid contents of starches from diploid cereals. J. Cereal Sci. 2:257–271.CrossRefGoogle Scholar
  15. Nakamura, T., Yamamori, M., Hirano, H., Hidaka, S., Nagamine, T. 1995. Production of waxy (amylose-free) wheats. Mol. Genet. Genom. 248:253–259.CrossRefGoogle Scholar
  16. Nakamura, T., Vrinten, P., Hayakawa, K., Ikeda, J. 1998. Characterization of a granule-bound starch synthase isoform found in the pericarp of wheat. Plant Physiol. 118:451–459.CrossRefGoogle Scholar
  17. Parker, M.L. 1985. The relationship between A-type and B-type starch granules in the developing endosperm of wheat. J. Cereal Sci. 3:271–278.CrossRefGoogle Scholar
  18. Peña, R.J., Trethowan, R., Pfeiffer, W.H., van Ginkel, M. 2002. Quality (end use) improvement in wheat: compositional, genetic, and environmental factors. J. Crop Prod. 5:1–37.CrossRefGoogle Scholar
  19. Sasaki, T., Yasui, T., Matsuki, J. 2000. Effect of amylose content on gelatinization, retrogradation and pasting properties of starches from waxy and nonwaxy wheat and their F1 seeds. Cereal Chem. 77:58–63.CrossRefGoogle Scholar
  20. Shin, M., Song, J., Seib, P.A. 2004. In vitro digestibility of cross-linked starches-RS4. Starch/Starke. 56:478–483.CrossRefGoogle Scholar
  21. Singh, S., Singh, N., Isono, N., Noda, T. 2010. Relationship of granule size distribution and amylopectin structure with pasting, thermal, and retrogradation properties in wheat starch. J. Agr. Food Chem. 58:1180–1188.CrossRefGoogle Scholar
  22. Stitt, M., Scheibe, R., Feil, R. 1989. Response of photosynthetic electron transport and carbon metabolism to a sudden decrease of irradiance in the saturating or the limiting range. BBA-Bioenergetics. 973:241–249.CrossRefGoogle Scholar
  23. Van Dongen, J.T., Roeb, G.W., Dautzenberg, M., Froehlich, A., Vigeolas, H., Minchin, P.E., Geigenberger, P. 2004. Phloem import and storage metabolism are highly coordinated by the low oxygen concentrations within developing wheat seeds. Plant Physiol. 135:1809–1821.CrossRefGoogle Scholar
  24. Wang, S.J., Wang, J.R., Zhang, W., Li, C.L., Yu, J.L., Wang, S. 2015. Molecular order and functional properties of starches from three waxy wheat varieties grown in China. Food Chem. 181:43–50.CrossRefGoogle Scholar
  25. Yu, X.R., Zhou, L., Jing, Y.P., Liu, D.T., Hu, M.L., Xiong, F., Wang, Z. 2013. Application of image-pro plus in analysis of wheat starch granule microscopic image. Journal of Chinese Electron Microscopy Society. 32:344–351. (In Chinese with English abstract)Google Scholar
  26. Yu, X.R., Yu, H., Zhang, J., Shao, S.S., Zhou, L., Xiong, F., Wang, Z. 2015. Comparison of endosperm starch granule development and physiochemical properties of starches from waxy and non-waxy wheat. Int. J. Food Prop. 18:2409–2421.CrossRefGoogle Scholar
  27. Zhang H.X., Zhang W, Xu C.Z., Zhou, X. 2013. Morphological features and physicochemical properties of waxy wheat starch. Int. J. Biol. Macromol. 62:304–309.CrossRefGoogle Scholar
  28. Zhu, T., Jackson, D.S., Wehling, R.L., Geera, B. 2007. Comparison of amylose determination methods and the development of a dual wavelength iodine binding technique. Cereal Chem. 85:51–58.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2018

Authors and Affiliations

  • H. Yu
    • 1
  • Y. Yang
    • 1
  • X. Y. Chen
    • 1
  • G. X. Lin
    • 1
  • J. Y. Sheng
    • 1
  • J. Y. Nie
    • 1
  • Q. J. Wang
    • 1
  • E. J. Zhang
    • 1
  • X. R. Yu
    • 1
  • Z. Wang
    • 1
  • F. Xiong
    • 1
    Email author
  1. 1.Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture & Agri-Product SafetyYangzhou UniversityYangzhouChina

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