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Cereal Research Communications

, Volume 46, Issue 2, pp 301–310 | Cite as

Detection and discrimination of barley- and wheat-specific forms of Wheat dwarf virus in Poland

  • K. TrzmieEmail author
Article

Abstract

Wheat dwarf virus (WDV) is one of the most common viruses on cereal crops in Poland. Studies were undertaken aiming at molecular characterization of Polish isolates of the virus. The presence of two main groups, WDV-barley- and WDV-wheat-specific forms, in field samples has been confirmed. Detection and differentiation of WDV isolates was conducted using immuno-capture polymerase chain reactions. The studies were carried out on a set of 68 samples collected from different parts of the country. Obtained results demonstrated that WDV-wheat-specific form can infect all tested cereals: wheat, triticale, rye and barley while WDV-barley-specific form was identified mainly in barley and in rare cases in wheat plants. Comparative analysis of coat protein gene was performed using 16 WDV isolates originated from different hosts revealed high (>98%) nucleotide sequence identity. Moreover, WDV-wheat-specific (Pol-WDV-W) and WDV-barley-specific (WDV-B) isolates were fully sequenced. Based on nucleotide sequence similarity, Pol-WDV-W should be classified as WDV-E and WDV-B as WDV-F strains. This is the first report of the complete sequence of WDV isolates from Poland.

Keywords

WDV wheat barley IC-PCR 

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References

  1. Achon, M.A., Serrano, L., Ratti, C., Rubies-Autonell, C. 2006. First detection of Wheat dwarf virus in barley in Spain associated with an outbreak of Barley Yellow Dwarf. Plant Dis. 90:970.CrossRefGoogle Scholar
  2. Áy, Z., Kerényi, Z., Takács, A., Papp, M., Petróczi, I.M., Gáborjányi, R., Silhavy, D., Pauk, J., Kertész, Z. 2008. Detection of cereal viruses in wheat (Triticum aestivum L.) by serological and molecular methods. Cereal Res. Commun. 36:215–224.CrossRefGoogle Scholar
  3. Behjatnia, S., Afsharifar, A., Tahan, V., Motlagh, M.A., Gandomani, O.E., Niazi, A., Izadpanah, K. 2011. Widespread occurrence and molecular characterization of Wheat dwarf virus in Iran. Aust. Plant Pathol. 40:12–19.CrossRefGoogle Scholar
  4. Bisztray, G., Gáborjányi, R., Vacke, J. 1989. Isolation and characterization of wheat dwarf virus found for the first time in Hungary. J. of Plant Dis. Prot. 96:449–454.Google Scholar
  5. Clark, M.F., Adams, A.N. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. of General Virol. 34:475–483.CrossRefGoogle Scholar
  6. Commandeur, U., Huth, W. 1999. Differentiation of strain of wheat dwarf virus in infected wheat and barley plants by means of polymerase chain reaction. J. of Plant Dis. Prot. 106:550–552.Google Scholar
  7. Ekzayez, A.M., Kumari, S.G., Ismail, I. 2011. First report of Wheat dwarf virus and its vector (Psammotettix provincialis) affecting wheat and barley crops in Syria. Plant Dis. 95:76.CrossRefGoogle Scholar
  8. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows95/98/NT. Nucleic Acids Symposium Series 41:95–98.Google Scholar
  9. Huth, V.W., Lessemann, D.E. 1994. Evidence of Wheat dwarf virus in Germany. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes 46:105–106.Google Scholar
  10. Jeżewska, M. 2001. First report of Wheat dwarf virus occurring in Poland. Phytopathologica Polonica 21:93–100.Google Scholar
  11. Jeżewska, M., Cajza, M., Buchowska-Ruszkowska, M. 2010. Monitoring i diagnostyka molekularna wirusów zbóż. In: Sosnowska, D. (ed), Ograniczanie strat w plonach roślin uprawnych z zachowaniem bezpieczeństwa żywności. Instytut Ochrony Roślin–PIB, Poznań, pp. 157–180. [Monitoring and molecular diagnostics of cereal viruses. In: Reducing losses in crop yields with food safety].Google Scholar
  12. Jeżewska, M., Trzmiel, K. 2016. Masowe wystąpienia żółtej mozaiki jęczmienia na zbożach ozimych w Polsce w sezonie 2014/2015. Progress in Plant Protection 56(3): 296–301.Google Scholar
  13. Jilaveanu, A., Vacke, J. 1995. Isolation and identification of wheat dwarf virus (WDV) in Romania. Probleme de Protectia Plantelor 23:51–62.Google Scholar
  14. Kapooria, R.G., Ndunguru, J. 2004. Occurrence of viruses in irrigated wheat in Zambia. EPPO Bulletin 34:413–419.CrossRefGoogle Scholar
  15. Köklü, G., Ramsell, J.N.E., Kvarnheden, A. 2007. The complete genome sequence for a Turkish isolate of Wheat dwarf virus (WDV) from barley confirms the presence of two distinct WDV strains. Virus Genes 34:359–366.CrossRefGoogle Scholar
  16. Kundu, J.K., Gadiou, S., Červená, G. 2009. Discrimination and genetic diversity of Wheat dwarf virus in the Czech Republic. Virus Genes 38:468–474.CrossRefGoogle Scholar
  17. Kvarnheden, A., Lindbland, M., Lindsten, K., Valkonen, J.P.T. 2002. Genetic diversity of Wheat dwarf virus. Archives of Virology 147:206–216.CrossRefGoogle Scholar
  18. Lemmetty, A., Huusela-Veistola, E. 2005. First report of WDV in winter wheat in Finland. Plant Dis. 89:912.CrossRefGoogle Scholar
  19. Lindbland, M., Sandgren, M., Sigvald, R. 1999. Epidemiology and control of wheat dwarf. In: Proc. VIIth Int. Plant Virus Epidemiology Symp. Aguadulce (Almeria), Spain. p. 114.Google Scholar
  20. Lindsten, K., Lindsten, B., Abdelmoeti, M., Juntti, N. 1980. Purification and some properties of wheat dwarf virus. In: Proc. 3rd Conf. on Virus Diseases of Gramineae in Europe. Rothamsted Experimental Station, Harpenden, Herts. pp. 27–31.Google Scholar
  21. Lindsten, K., Lindsten, B. 1993. Occurrence and transmission of Wheat dwarf virus (WDV) in France. In: A.N.P.P. Third Int. Conf. on Pests in Agriculture. Montpellier, France. pp. 41–48.Google Scholar
  22. Lindsten, K., Vacke, J. 1991. A possible barley adapted strain of Wheat dwarf virus (WDV). Acta Phytopathologica et Entomologica Hungarica 26:175–180.Google Scholar
  23. Mehner, S., Manurung, B., Grüntzig, M., Habekuß, A., Witsack, W., Fuchs, E. 2003. Investigations into the ecology of the Wheat dwarf virus (WDV) in Saxony-Anhalt, Germany. J. of Plant Dis. Prot. 110:313–323.Google Scholar
  24. Muhire, B., Martin, D.P., Brown, J.K., Navas-Castillo, J., Moriones, E., Zerbini, F.M., Rivera-Bustamante, R., Malathi, V.G., Briddon, R.W., Varsani, A. 2013. A genome-wide pairwise-identity-based proposal for the classification of the viruses in the genus Mastrevirus (family Geminiviridae). Archives of Virology 158:1411–1424.CrossRefGoogle Scholar
  25. Najar, A., Makkouk, K.M., Kumari, S.G. 2000. First record of Barley yellow striate mosaic virus, Barley stripe mosaic virus, and Wheat dwarf virus infecting cereal crops in Tunisia. Plant Dis. 84:1045.CrossRefGoogle Scholar
  26. Rabenstein, F., Sukhacheva, E., Habekuß, A., Schubert, J. 2005. Differentiation of Wheat dwarf virus isolates from wheat, triticale and barley by means of a monoclonal antibody. In: Proc. Xth Conf. on Viral Diseases of Gramineae in Europe. Louvain-la-Neuve, Belgium. p. 60.Google Scholar
  27. Ramsell, J.N.E., Lemmetty, A., Jonasson, J., Andersson, A., Sigvald, R., Kvarnheden, A. 2008. Sequence analyses of Wheat dwarf virus isolates from different hosts reveal low genetic diversity within the wheat strain. Plant Pathol. 57:834–841.CrossRefGoogle Scholar
  28. Rosen, S., Skaletski, H.J. 2000. Primer3 on the WWW for general use and for biologist programmers. In: Krawetz, S. Misenes, S. (eds), Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press. Totova/New Jersey, USA. pp. 365–386.Google Scholar
  29. Schubert, J., Habekuß, A., Kazmaier, K., Jeske, H. 2007. Surveying cereal-infecting geminiviruses in Germany – Diagnostic and direct sequencing using rolling circle amplification. Virus Res. 127:61–70.CrossRefGoogle Scholar
  30. Schubert, J., Habekuß, A., Wu, B., Thieme, T., Wang, X. 2014. Analysis of complete genomes of isolates of the Wheat dwarf virus from new geographical locations and descriptions of their defective forms. Virus Genes 48:133–139.CrossRefGoogle Scholar
  31. Snihur, H., Polischuk, V., Kastirr, U. 2007. Dissemination of viruses of cereal crops in agrocoenosises of Ukraine. In: 10th International Plant Virus Epidemiology Symp. ICRISAT. Hyderabad, India. p. 107.Google Scholar
  32. Stephanov, J., Dimov, A. 1981. Bolestta vdjudjavanje po spenittsata Bolgaria. Rasteniev Nauki 18:1274–128. [The Wheat dwarf disease in Bulgaria.] Tóbiás, I., Kiss, B., Bakardjieva, N., Palkovics, L. 2009. The nucleotide sequence of barley strain of of Wheat Dwarf Virus isolated in Bulgaria. Cereal Res. Commun. 37:237–242.Google Scholar
  33. Tóbiás, I., Shevchenko, O., Kiss, B., Bysov, A., Snihur, H., Polischuk, V., Salanki, K., Palkovics, L. 2011. Comparison of the nucleotide sequences of Wheat Dwarf Virus (WDV) isolates from Hungary and Ukraine. Polish J. of Microbiol. 60:125–131.Google Scholar
  34. Vacke, J. 1961. Wheat dwarf virus disease. Biologia Plantarum (Praha) 3:228–233.CrossRefGoogle Scholar
  35. Vacke, J. 1962. Some new findings on what dwarf virus. In: Plant Virology, Proc. 5th Conference of the Czechoslovak Plant Virologists. Publishing House of the Czechoslovak Academy of Sciences. Prague, Czechoslovakia. pp. 331–334.Google Scholar
  36. Vacke, J., Kvarnheden, A., Lindbland, M., Lindsten, K. 2004. Wheat Dwarf. In: Lapierre, H., Signoret, P.A. (eds), Viruses and Virus Diseases of Poaceae (Gramineae). INRA. Paris, France. pp. 590–593.Google Scholar
  37. Wu, B., Shang, X., Shubert, J., Habekuß, A., Elena, S.F., Wang, X. 2015. Global-scale computational analysis of genomic sequences reveals the recombination pattern and coevolution dynamics of cereal-infecting geminiviruses. Scientific Reports 5:e 8153.CrossRefGoogle Scholar
  38. Xie, J., Wang, X., Liu, Y., Peng, Y., Zhou, G. 2007. First report of the occurrence of Wheat dwarf virus in wheat in China. Plant Dis. 91:111.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2018

Authors and Affiliations

  1. 1.Department of Virology and BacteriologyInstitute of Plant Protection — National Research InstitutePoznańPoland

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