Cereal Research Communications

, Volume 46, Issue 2, pp 287–300 | Cite as

Influence of Proline Priming on Antioxidative Potential and Ionic Distribution and its Relationship with salt Tolerance of Wheat

  • F. ShafiqEmail author
  • S. H. Raza
  • A. Bibi
  • I. Khan
  • M. Iqbal


Mechanisms involved in salt tolerance urge exploration and investigation of genotypic variation to assist future breeding programs. Comparative examination of ten wheat cultivars for salt tolerance and their response towards proline-seed-priming was performed. Exposure of wheat seedlings to salinity resulted in prominent reduction in root and shoot growth attributes of all cultivars. Furthermore, decrease in the chlorophyll contents was evident although this varied among cultivars. Wheat seedlings grown from proline pre-treated seeds exhibited improved photosynthetic pigments, besides this response was also cultivar and concentration dependent. Generally, salt stressed plants exhibited higher antioxidant enzyme activities. Proline priming significantly influenced antioxidant activities, however, its magnitude varied. The peroxidase activity varied among wheat cultivars that were evident from the analysis of POD activity on Native-PAGE gel. Salinity caused the accumulation of Na+ in the roots and the magnitude of Na+ translocation to the shoot was cultivar dependent. Similarly, K+ uptake and its distribution among root and shoot varied. Priming treatments affected ion distribution of Na+ and K+ but inter-cultivar variations were evident. Conclusively, all the cultivars investigated exhibited differential response to salinity and proline seed pre-treatments. However, the proline-priming mediated improvements in growth and antioxidant enzyme activities contributed to stress tolerance which partly relied on the ability of the plant to uptake sodium and its partitioning in the roots. Of the cultivars tested, Faisalabad-08 and Bhakhar-2002 were ranked as relatively salt tolerant and the cvs. AARI-10, MH-97 and Auqab-2000 as relatively salt sensitive.


catalase peroxidase proline priming salinity superoxide dismutase wheat 


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  1. Aebi, H. 1984. Catalase. In: L. Packer (ed.), Methods in enzymology, Academic Press.Orlando, FL, USA. 105:121–126.Google Scholar
  2. Alscher, R.G., Erturk, N., Heath, L.S. 2002. Role of superoxide dismutases (SODs) in controlling oxidative stress. J. Exp. Bot. 53:1331–1341.CrossRefGoogle Scholar
  3. Apel, K., Hirt, H. 2004. Reactive oxygen species, metabolism, oxidative stress and signal transduction. Ann. Review Plant Biol. 55:373–399.CrossRefGoogle Scholar
  4. Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24:1–10.CrossRefGoogle Scholar
  5. Averinaa, N.G., Gritskevicha, E.R., Vershilovskayaa, I.V., Usatovb, A.V., Yaronskaya, E.B. 2010. Mechanisms of salt stress tolerance development in barley plants under the influence of 5-amino-levulinic acid. Russ. J. Plant Physiol. 57:792–798.CrossRefGoogle Scholar
  6. Chance, B., Maehly, A.C. 1955. Assay of catalases and peroxidases. Meth. Enzymol. 2:764–775.CrossRefGoogle Scholar
  7. Daliakopoulos, I.N., Tsanis, I.K., Koutroulis, A., Kourgialas, N.N., Varouchakis, A.E., Karatzas, G.P., and Ritsema, C.J. 2016. The threat of soil salinity: A European scale review. Sci. Total Environ. 573:727–739.CrossRefGoogle Scholar
  8. Dhanda, S.S., Sethi, G.S., Behl, R.K. 2004. Indices of drought tolerance in wheat genotypes at early stages of plant growth. J. Agron. Crop Sci. 190:6–12.CrossRefGoogle Scholar
  9. FAO 2008. FAO land and plant nutrition management service.
  10. Forni, C., Duca, D., Glick, B.R. 2017. Mechanisms of plant response to salt and drought stress and their alteration by rhizobacteria. Plant Soil. 410:335–356.CrossRefGoogle Scholar
  11. Giannopolitis, C.N, Ries, S.K. 1977. Superoxide dismutases, purification and quantitative relationship with water-soluble protein in seedlings. Plant Physiol. 59:315–318.CrossRefGoogle Scholar
  12. Hernández, J., Jimenez, A., Mullineaux, P., Sevilla, F. 2000. Tolerance of pea plants (Pisum sativum) to long term salt stress is associated with induction of antioxidant defenses. Plant Cell Environ. 23:853–862.CrossRefGoogle Scholar
  13. Hernandez, M., Garcia, N.F., Vivancos, P.D., Olmos, E. 2009. A different role for hydrogen peroxide and the antioxidative system under short and long salt stress in Brassica oleracea roots. J. Exp. Bot. 61:521–535.CrossRefGoogle Scholar
  14. Iqbal, M., Ashraf, M. 2013. Gibberellic acid mediated induction of salt tolerance in wheat plants: Growth, ionic partitioning, photosynthesis, yield and hormonal homeostasis. Environ. Exp. Bot. 86:76–85.CrossRefGoogle Scholar
  15. Koevoets, I.T., Venema, J.H., Elzenga, J.T.M., Testerink, C. 2016. Roots withstanding their environment: exploiting root system architecture responses to abiotic stress to improve crop tolerance. Frontiers Plant Sci. 1–7.Google Scholar
  16. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685.CrossRefGoogle Scholar
  17. Miller, G., Honig, A., Stein, H., Suzuki, N., Mittler, R., Zilberstein, A. 2009. Unraveling delta1-pyrroline-5-carboxylate (P5C)/proline cycle in plants by uncoupled expression of proline oxidation enzymes. J. Biol. Chem. 284:26482–26492.CrossRefGoogle Scholar
  18. Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7:405–410.CrossRefGoogle Scholar
  19. Mittler, R. 2006. Abiotic Stress, the field environment and stress combination. Trends Plant Sci. 11:15–19.CrossRefGoogle Scholar
  20. MSTAT Development Team 2013. MSTAT User’s Guide: A Microcomputer Program for the Design Management and Analysis of Agronomic Research Experiments. Michigan State University. East Lansing, MC, USA.Google Scholar
  21. Munns, R, James, R.A. 2003. Screening methods for salinity tolerance: A case study with tetraploid wheat. Plant Soil 253:201–218.CrossRefGoogle Scholar
  22. Munns, R, Tester, M. 2008. Mechanisms of salinity tolerance. Ann. Rev. Plant. Biol. 59:651–681.CrossRefGoogle Scholar
  23. Munns, R. 2002. Comparative physiology of salt and water stress. Plant Cell Environ. 25:239–250.CrossRefGoogle Scholar
  24. Munns, R., James, R.A., Xu, B., Athman, A., Conn, S.J., Jordans, C., Byrt, C.S., Hare, R.A., Tyerman, S.D., Tester, M., Plett, D., Gilliham, M. 2012. Wheat grain yield on saline soils is improved by an ancestral Na+ transporter gene. Nature Biotech. 30:360–364.CrossRefGoogle Scholar
  25. Murtaza, B., Murtaza, G., Sabir, M., Owens, G., Abbas, G., Imran, M., Shah, G.M. 2017. Amelioration of saline–sodic soil with gypsum can increase yield and nitrogen use efficiency in rice–wheat cropping system. Arch. Agron. Soil Sci. 63:1267–1280.CrossRefGoogle Scholar
  26. Raza, S.H., Athar, H.R., Ashraf, M., Hameed, A. 2007. Glycine betaine-induced modulation of antioxidant enzymes activities and ion accumulation in two wheat cultivars differing in salt tolerance. Environ. Exp. Bot. 3:368–376.CrossRefGoogle Scholar
  27. Raza, S.H., Ahmad, M.B., Ashraf, M.A., Shafiq, F. 2014. Time-course changes in growth and biochemical indices of mung bean [Vigna radiata (L.) Wilczek] genotypes under salinity. Braz. J. Bot. 37:429–439.CrossRefGoogle Scholar
  28. Robin, A.H.K., Matthew, C., Uddin, M.J., Bayazid, K.N. 2016. Salinity-induced reduction in root surface area and changes in major root and shoot traits at the phytomer level in wheat. J. Exp. Bot. 67:3719–3729.CrossRefGoogle Scholar
  29. Roy, S., Negrao, S., Tester, M. 2014. Salt resistant crop plants. Curr. Opinion Biotech. 26:115–124.CrossRefGoogle Scholar
  30. Schleiff, U. 2008. Analysis of water supply of plants under saline soil conditions and conclusions for research on crop salt tolerance. J. Agron. Crop. Sci. 194:1–8.CrossRefGoogle Scholar
  31. Sileshi, A.A., Kibebew, K. 2016. Status of salt affected soils, irrigation water quality and land suitability of Dubti/Tendaho area, North Eastern Ethiopia. Doctoral dissertation, Haramaya University. Alemaya, Ethiopia.Google Scholar
  32. Szabados, L.S., Savoure, A. 2009. Proline, a multifunctional amino acid. Trends Plant Sci. 15:89–97.CrossRefGoogle Scholar
  33. Ueda, A., Yamamoto-Yamane, Y., Takabe, T. 2007. Salt stress enhances proline utilization in the apical region of barley roots. Biochem. Biophysics Res. Commun. 355:61–66.CrossRefGoogle Scholar
  34. Van Loon, L.C. 1971. Tobacco polyphenoloxidase: A specific staining method indicating non-identify with peroxidases. Phytochem. 10:503–507.CrossRefGoogle Scholar
  35. Wolf, B.A. 1982. Comprehensive system of leaf analysis and its use for diagnosing crop nutrients status. Commun. Soil Sci. Plant Anal. 13:1035–1059.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2018

Authors and Affiliations

  • F. Shafiq
    • 1
    Email author
  • S. H. Raza
    • 1
  • A. Bibi
    • 1
  • I. Khan
    • 1
  • M. Iqbal
    • 1
  1. 1.Department of BotanyGovernment College University FaisalabadPunjabPakistan

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