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Cereal Research Communications

, Volume 47, Issue 1, pp 98–110 | Cite as

Ammonium Uptake and Assimilation are Required for Rice Defense against Sheath Blight Disease

  • W. J. Chi
  • Z. Y. Wang
  • J. M. Liu
  • C. ZhangEmail author
  • Y. H. WuEmail author
  • Y. J. BaiEmail author
Pathology

Abstract

Nitrogen (N) is an important nutrient for plant growth and yield production, and rice grown in paddy soil mainly uses ammonium (NH4+) as its N source. Previous studies have shown that N status is tightly connected to plant defense; however, the roles of NH4+ uptake and assimilation in rice sheath blight disease response have not been studied previously. Here, we analyzed the effects of different N sources on plant defense against Rhizoctonia solani. The results indicated that rice plants grown in N-free conditions had higher resistance to sheath blight than those grown under N conditions. In greater detail, rice plants cultured with glutamine as the sole N source were more susceptible to sheath blight disease compared to the groups using NH4+ and nitrate (NO3) as sole N sources. N deficiency severely inhibited plant growth; therefore, ammonium transporter 1;2 overexpressors (AMT1;2 OXs) were generated to test their growth and defense ability under low N conditions. AMT1;2 OXs increased N use efficiency and exhibited less susceptible symptoms to R. solani and highly induced the expression of PBZ1 compared to the wild-type controls upon infection of R. solani. Furthermore, the glutamine synthetase 1;1 (GS1;1) mutant (gs1;1) was more susceptible to R. solani infection than the wild-type control, and the genetic combination of AMT1;2 OX and gs1;1 revealed that AMT1;2 OX was less susceptible to R. solani and required GS1;1 activity. In addition, cellular NH4+ content was higher in AMT1;2 OX and gs1;1 plants, indicating that NH4+ was not directly controlling plant defense. In conclusion, the present study showed that the activation of NH4+ uptake and assimilation were required for rice resistance against sheath blight disease.

Keywords

AMT assimilation sheath blight defense GS1;1 rice 

Abbreviations

AMT

ammonium transporter

Os

Oryza sativa

GS

glutamine syn-thetase

N

nitrogen

R. solani

Rhizoctonia solani

OX

overexpression

ORF

open reading frame

PBZ1

Probenazole-inducible gene

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Supplementary material

42976_2019_4701098_MOESM1_ESM.pdf (109 kb)
Ammonium Uptake and Assimilation are Required for Rice Defence against Sheath Blight Disease

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Copyright information

© Akadémiai Kiadó, Budapest 2019

Authors and Affiliations

  1. 1.Institute of Plant ProtectionLiaoning Academy of Agricultural SciencesShenyangChina
  2. 2.College of Plant ProtectionShenyang Agricultural UniversityShenyangChina
  3. 3.College of Life EngineeringShenyang Institute of TechnologyFushunChina
  4. 4.Department of Agricultural and Biological TechnologyWenZhou Agricultural Science Research Institute (WenZhou Vocational College of Science &Technology)WenzhouChina

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