This experiment was carried out to evaluate the growth, physiological and yield traits of stay-green (Stg) QTL introgression sorghum lines, which were developed by the Ethiopian Institute of Agricultural Research in collaboration with ICRISAT between 2006 and 2008, under induced post-flowering drought stress. It involved a total of 12 genotypes including seven Stg QTL introgression lines, two Stg donor parents and three senescent recurrent parents. It was organized in a split plot design with three replications under well-watered and induced drought stress growing conditions at Melka Werer, Ethiopia during the post-rainy season of 2014. Analysis of variance revealed that the effect of moisture regimes on all measured traits was significant (P < 0.05). Differences among the genotypes and genotypeby-water regime interaction were also significant (P < 0.05) for all the traits considered. Post-flowering drought stress was observed to significantly reduce most of the growth, physiological and yield related traits. The Stg introgression lines Meko/B35-selection 120, Teshale/ B35-selection 2 and Teshale/E36-1 showed better drought stress tolerance properties than the rest of the genotypes based on the measured growth and physiological traits. These introgression lines also showed better grain yield than their recurrent parents under post-flowering drought stress and can be used as new versions of the existing varieties (served as recurrent parents) and for future breeding programs. Furthermore, leaf chlorophyll content, assimilation rate, transpiration rate, water use efficiency, root length and root dry weight were found to have strong correlation and can be used to screen genotypes for post-flowering drought tolerance.
Abdullah, F., Hareri, F., Naaesan, M., Ammar, M.A., Kanbar, O.Z. 2011. Effect of drought on different physiological characters and yield component in different varieties of Syrian durum wheat. J. of Agric. Sci. 3:127–133.
Adugna, A. 2007. The role of introduced sorghum and millets in Ethiopian agriculture. SAT e-Journal 3:1–4.
Adugna, A., Tirfessa, A. 2014. Response of stay-green quantitative trait locus (QTL) introgression sorghum lines to post-anthesis drought stress. African J. of Biotechnol. 13:4492–4500.
Atta, B.M., Mahmood, T., Trethowan, R.M. 2013. Relationship between root morphology and grain yield of wheat in north-western NSW, Australia. Aust. J. of Crop Sci. 7:2108–2115.
Barrs, H.D. 1968. Determination of water deficits in plant tissue. In: Kozlowski, T.T. (ed.) Water Deficits and Plant Growth. Academic Press. New York, USA. vol. 1, pp. 235–368.
Bezabih, A. 2012. Evaluation of stay-green quantitative trait loci (QTLs) introgression sorghum (Sorghum bicolor (L.) Moench) lines for post-flowering drought resistance at Kobo, North eastern Ethiopia. M.Sc. Thesis, Haramaya University. Alemaya, Ethiopia.
Bibi, A., Sadaqat, H.A., Tahir, M.H.N., Akram, H.M. 2012. Screening of sorghum (Sorghum bicolor var Moench) for drought tolerance at seedling stage in polyethylene glycol. The J. of Animal and Plant Sci. 22:671–678.
Blum, A. 2005. Drought resistance, water-use efficiency, and yield potential are they compatible, dissonant, or mutually exclusive? Aust. J. of Agric. Res. 56:1159–1168.
Borrell, A., Hammer, G., Van Oosterom, E. 2001. Stay-green: A consequence of the balance between supply and demand for nitrogen during grain filling? Ann. Appl. Biol. 138:91–95.
Borrell, A.K., Bidinger, F.R., Sunitha, K. 1999. Stay-green associated with yield in recombinant inbred sorghum lines at varying rate of leaf senescence. ISML 40:31–33.
Borrell, A.K., Hammer, G.L. 2000. Nitrogen dynamics and the physiological basis of stay-green in sorghum. Crop Sci. 40:1295–1307.
Campanile, R.G., DeVita, P., DiFonzo, N., Flagella, Z., Laus, M., Lawlor, D., Padalino, L., Rascio, A., Russo, M., Shewry, P.R., Spano, G., Stoppelli, M.C., Troccoli, A., Trono, D. 2000. Resistance to a biotic stresses in durum wheat: Which ideotype? In: Royo, C., Nachit, M., DiFonzo, N., Araus, J.L. (eds), Durum Wheat Improvement in the Mediterranean Region: New Challenges. CIHEAM. Zaragoza, Spain. pp. 215–225.
Dial, H.L. 2012. Plant Guide for Sorghum (Sorghum bicolor L.). USDA-Natural Resources Conservation Service, Tucson Plant Materials Center. Tucson, AZ, USA.
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., Basra, S.M.A. 2009. Plant drought stress: effects mechanisms and management. Agronomy for Sustainable Devel. 29:185–212.
Hasheminasab, H., Assad, M.T., Aliakbari, A., Sahhafi, S.R. 2012. Evaluation of some physiological traits associated with improved drought tolerance in Iranian wheat. Ann. of Biol. Res. 3:1719–1725.
IBPGR and ICRISAT. 1993. Descriptors for sorghum [Sorghum bicolor (L.) Moench]. International Board of Plant Genetic Resources, Rome, Italy. ICRISAT, Patacheru, India.
Kamran, M., Naeem, M.K., Ahmad, M., Nawazshah, M.K., Iqbal, M.S. 2014. Physiological responses of wheat (Triticum aestivum L.) against drought stress. Am. J. Res. Commun. pp. 1–9. http://www.usa-journals.com. ISSN:2325–4076.
Kapanigowda, M.H., Payne, W.A., Rooney, W.L., Mullet, J.E. 2012. Transpiration ratio in sorghum [Sorghum bicolor (L.) Moench] for increased water-use efficiency and drought tolerance. J. of Arid Land Studies 22:175–178.
Kassahun, B., Bidinger, F.R., Hash, C.T. 2010. Stay-green expression in early generation sorghum (Sorghum bicolor (L.) Moench) QTL introgression lines. Euphytica 172:351–362.
Khakwani, A.A., Dennett, M.D., Munir, M., Baloch, M.S. 2012. Wheat yield response to physiological limitations under water stress condition drought resistance mechanisms. The J. of Animal and Plant Sci. 22:773–780.
Mafakheri, A., Siosemardeh, B., Bahramnejad, B., Struik, P.C., Sohrab, Y. 2010. Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Aust. J. of Crop Sci. 4:580–585.
Malala, T.J. 2010. Evaluation and selection of 20 Sorghum (Sorghum bicolor (L.) Moench) genotypes for drought tolerance. M.Sc. Thesis, University of Pretoria. Pretoria, South-Africa.
Prasad, P.V.V., Staggenborg, S.A., Ristic, Z. 2008. Response of crops to limited water. In: Royo, C., Nachit, M., DiFonzo, N., Araus, J.L. (eds), Understanding and Modeling Water Stress Effects on Plant Growth Processes: New Challenges. CIHEAM. Zaragoza, Spain. pp. 215–236.
Reddy, N., Reddy, R., Ragimasalawada, M., Sabbavarapu, M.M., Nadoor, S., Patil, J.V. 2014. Detection and validation of stay-green QTL in post-rainy sorghum involving widely adapted cultivar, M35–1 and a popular stay-green genotype B35. BMC Genomics 15:2–16.
Rosenow, D.T., Quisenberry, J.E., Wendt, C.W. 1983. Drought tolerant sorghum and cotton germplasm. Agric. Water Management 7:207–222.
Rostampour, M.F., Yarnia, R.M., Khoee, F., Seghatoleslami, M.J., Moosavi, G.R. 2012. Effect of superb A200 and drought stress on dry matter yield in forage Sorghum. Am.-Eurasian J. Agric. Env. Sci. 12:231–236.
Shamsi, K., Petrosyan, M., Mohammadi, N.G., Haghparast, R. 2010. Evaluation of grain yield and its components in three bread wheat cultivars under drought stress. J. of Animal and Plant Sci. 9:1117–1121.
Songsri, P., Jogloy, S., Holbrook, C.C., Kesmala, T., Vorasoot, N., Akkasaeng, C., Patanothai, A. 2009. Association of root, specific leaf area and SPAD chlorophyll meter reading to water use efficiency of peanut under different available soil water. Agric. Water Management 96:790–798.
Soo-Cheul, Y., Sung-Hwan, C., Zhang, H., Hyo-Chung, P., Chung-Hee, L., Jinjie, L., Jeong-Hoon, Y., Byun-Woo, L., Hee-Jong, K., Seo, H.S., Nam-Chon, P. 2007. Quantitative trait loci associated with functional stay-green SNU-SG1 in Rice. Molecules and Cells 24:83–94.
Sticker, F.C., Wearden, S., Pauli, A.W. 1961. Leaf area determination in grain sorghum. Agron. J. 53:187–188.
Taiz, L., Zeiger, E. 2006. Plant Physiology. Sinauer Associates, Inc. Sunderland, UK. pp. 591–602.
Talebi, R., Ensafi, M.H., Baghebani, N., Karami, E., Mohammadi, K. 2013. Physiological responses of chickpea (Cicer arietinum) genotypes to drought stress. Environ. and Exp. Biol. 11:9–15.
Thevar, P.A., Kirkham, M.B., Aiken, R.M., Kofoid, K.D., Xin, Z. 2010. Optimizing water use with high-transpiration-efficiency plants. 19th World Cong. of Soil Science, Soil Solutions for a Changing World. Brisbane, Australia. pp. 804–807.
Xu, W., Subudhi, P.K., Crasta, O.R., Rosenow, D.T., Mullet, J.E., Nguyen, H.T. 2000. Molecular mapping of QTLs conferring stay green in sorghum. Genome 43:461–469.
Yamasaki, S., Dillenburg, L.R. 1999. Measurements of leaf relative water content in Araucaria angustifolia. Revista Brasilleira de Fisiologia Vegetal 11: 69–75.
We thank the national sorghum research program of the Ethiopian Institute of Agricultural Research (EIAR) at Melkassa Agricultural Research Center, Adama for providing with the experimental materials. The Department of Plant Biology and Biodiversity Management of Addis Ababa University is also hereby acknowledged for the laboratory space and equipment.
Communicated by A. Börner
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Sintayehu, S., Adugna, A., Fetene, M. et al. Study of Growth and Physiological Characters in Stay-green QTL Introgression Sorghum bicolor (L.) Lines under Post-flowering Drought Stress. CEREAL RESEARCH COMMUNICATIONS 46, 54–66 (2018). https://doi.org/10.1556/0806.45.2017.061
- QTL introgression lines