Effect of Cerium (Ce) on the Redox States of Ascorbate and Glutathione through Ascorbate-glutathione Cycle in the Roots of Maize Seedlings under Salt Stress

Abstract

This study investigated the effect of cerium (Ce) on the ascorbate-glutathione (AsA-GSH) cycle in the roots of maize seedlings under salt stress. The results showed that salt stress significantly increased the activities of ascorbate peroxidase (APX) and monodehydroascorbate reductase (MDHAR), malondialdehyde (MDA) content and electrolyte leakage (EL) in the roots of maize seedlings, compared to the control. However, salt stress significantly decreased plant height, dry weight of root and shoot, the activities of glutathione reductase (GR) and dehydroascorbate reductase (DHAR), and the ratios of AsA/DHA and GSH/GSSG in the roots of maize seedlings, compared to the control. Pretreatment with cerium nitrate (Ce(NO3)3) significantly increased plant height, dry weight of root and shoot, the activities of above four enzymes, and the ratios of AsA/DHA and GSH/GSSG under salt stress, compared to salt stress alone. Pretreatment with Ce(NO3)3 significantly decreased MDA content and EL induced by salt stress, compared to salt stress alone. Meanwhile, application of Ce(NO3)3 to the control also significantly increased plant height, dry weight of root and shoot, the activities of above four enzymes and the ratios of AsA/DHA and GSH/GSSG, and decreased MDA content and EL, compared with control. Our results suggested that Ce(NO3)3 alleviated salt stress-induced oxidative damage by improving AsA-GSH cycle in maize roots.

Abbreviations

APX:

ascorbate peroxidase

GR:

glutathione reductase

DHAR:

dehydroascorbate reductase

MDHAR:

monodehydroascorbate reductase

GSH:

reduced glutathione

AsA:

reduced ascorbic acid

MDA:

malondialdehyde

EL:

electrolyte leakage

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Acknowledgement

This study was funded by the Science and Technology Program of Henan Province (142102110041).

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Correspondence to C. Shan.

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Communicated by S. Gottwald

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Hu, H., Shan, C. Effect of Cerium (Ce) on the Redox States of Ascorbate and Glutathione through Ascorbate-glutathione Cycle in the Roots of Maize Seedlings under Salt Stress. CEREAL RESEARCH COMMUNICATIONS 46, 31–40 (2018). https://doi.org/10.1556/0806.45.2017.057

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Keywords

  • salt stress
  • cerium nitrate
  • ascorbate-glutathione cycle
  • maize
  • root