Advertisement

Cereal Research Communications

, Volume 45, Issue 2, pp 192–201 | Cite as

Salicylic Acid and Fish Flour Pre-treatments Affect Wheat Phenolic and Flavonoid Compounds, Lipid Peroxidation Levels under Salt Stress

  • B. Karadağ
  • N. Candan YücelEmail author
Article

Abstract

The potential effect of combined salicylic acid and fish flour to improve plant tolerance to salt stress was investigated. This pre-treatment improved the growth of wheat seedlings under salinity when compared to control (untreated wheat seedlings). Moreover, combined pre-treatment improved significantly phenylalanine ammonia lyase (PAL) and peroxidase (POD) enzyme activities, also phenolic-flavonoid content in the shoots of salt stressed seedlings. One of the most important consequences of increase in salt stress is the oxidative tissue damage. In our study, salt stress increased lipid peroxidation levels (LPO) and also the loss of chlorophylls levels during stress might also be related to photo-oxidation resulting from oxidative stress. Whereas phenylalanine ammonia-lyase (PAL) activities of wheat shoots increased by a 2.1-fold under salt stress, the activities of shoots grown from seeds primed with salicylic acid and fish flour (SA + FF) increased by a 4-fold for 0.05 mM SA + FF, 4.8- fold for 0.1 mM SA + FF and 3.7-fold for 2.5 mM SA + FF combined pre-treatment under salt stress. Also, the combined salicylic acid + fish flour primed seedlings showed higher content of the scopoletin, and salicylic, syringic, vanilic and gallic acids under both salt and non-salinity stress conditions.

Keywords

phenolic contents fish flour salicylic acid salt stress wheat 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anonymus 2000. Fisheries statistics. State Institute of Statistics, Prime Ministry Republic of Turkey. Ankara, Turkey.Google Scholar
  2. Arora, A., Byrem, T.M., Nair, M.G., Strasburg, G.M., 2000. Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Arch. Biochem. Biophys. 373:102–109.CrossRefGoogle Scholar
  3. Azooz, M.M. 2009. Salt stress mitigation by seed priming with salicylic acid in two faba bean genotypes in salt tolerance. Inter. J. Agric. Biol. 11:343–350.Google Scholar
  4. Boscolo, W.R. 2001. Desempenhoe caracteristicas de carcaqa de machos revertidos de tilapias do Nilo (Oreochromis niloticusL.) (Performance and carcass characteristics of Nile Tilapia (Oreochromis niloticusL.) fed with rations containing different levels of fat). Revista Brasileire Zootecnol. 30:1391–1396. (in Spanish)CrossRefGoogle Scholar
  5. Bradford, K.J. 1976. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortSci. 21:1105–1112.Google Scholar
  6. Buege, J.A., Aust, S.D. 1978. Microsomal lipid peroxidation. Methods Enzymol. 52:302–310.CrossRefGoogle Scholar
  7. Dincer, T., Cakli, S., Kilinc, B., Tolasa, S. 2010. Amino acids and fatty acid composition content of fish sauce. J. Animal Vet. Advan. 9:311–315.CrossRefGoogle Scholar
  8. Du, L., Ali, G.S., Simons, K.A., Hou, J., Yang, T., Reddy, A.S., Poovanah, B.W. 2009. Ca2+/calmodulin regulates salicylic-acid-mediated plant immunity. Nature 457:1154–1158.CrossRefGoogle Scholar
  9. Hodgins, D.S. 1971. Yeast phenylalanine ammonia-lyase. Purification, properties, and the identification of catalytically essential dehydroalanine. J. Biol. Chem. 46:2977–2985.Google Scholar
  10. Horii, A., Mccue, P., Shetty, K. 2007. Enhancement of seed vigour following insecticide and phenolic elicitor treatment. Biores. Technol. 98:623–632.CrossRefGoogle Scholar
  11. Iyengar, E.R.R., Reddy, M.P. 1996. Photosynthesis in highly salt tolerant plants. In: Pesserkali, M. (ed.), Handbook of Photosynthesis. Marshal Dekar. Baten Rose, USA. pp. 897–909.Google Scholar
  12. Lan, T., You, J., Kong, L., Yu, M., Liu, M., Yang, Z. 2016. The interaction of salicylic acid and Ca alleviates aluminium toxicity in soybean. Plant Physiol. Biochem. 98:146–154.CrossRefGoogle Scholar
  13. Lee, J., Scagel, C.F. 2009. Chicoric acid found in basil (Ocimum basilicum L.) leaves. Food Chem. 115:650–656.CrossRefGoogle Scholar
  14. Lichtenthaler, H.K. Wellburn, A.R. 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans. 11:591–592.CrossRefGoogle Scholar
  15. Manaa, A., Gharbi, E., Mimouni, H., Wasti, S., Aschi-Smiti, S., Lutts, S., Ben Ahmed, H. 2014. Simultaneous application of salicylic acid and calcium improves salt tolerance in two contrasting tomato cultivars. South Afric. J. Bot. 95:32–39.CrossRefGoogle Scholar
  16. McCue, P., Zheng, Z., Pinkham, J.L., Shetty, K. 2000. A model for enhanced pea seedling vigour following low pH and salicylic acid treatments. Process Biochem. 35:600–613.CrossRefGoogle Scholar
  17. Miladinov, Z.J., Balesevic-Tubic, S.N., Ðordevic, V.B., Ðukic, V.H., Ilic, A.D., Cobanovic, L.M. 2015. Optimal time of soybean seed priming and primer effect under salt stress conditions. J. Agric. Sci. Belgrad 60:109–117.CrossRefGoogle Scholar
  18. Milic, B.L., Djilas, S.M., Canadanovic-Brunet, J.M. 1998. Antioxidative activity of phenolic compounds. Food Chem. 61:443–447.CrossRefGoogle Scholar
  19. Nakano, Y., Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22:867–880.Google Scholar
  20. Naz, F., Gul, H., Hamayun, M., Sayyed, A., Khan, H., Sherwani, S. 2014. Effect of NaCl stress on P. sativumgermination and seedling growth with the influence ofseed priming with potassium (KCL and KOH). Am.- Eur. J. Agric. Environ. Sci. 14:1304–1311.Google Scholar
  21. Randhir, R., Shetty, K. 2003. Light-mediated fava bean (Vicia faba) response to phytochemical and protein elicitors and consequences on nutraceutical enhancement and seed vigour. Process Biochem. 38:945–952.CrossRefGoogle Scholar
  22. Saleh, A.M., Madany, M.M.Y. 2015. Coumarin pretreatment alleviates salinity stress in wheat seedlings. Plant Physiol. Biochem. 88:27–35.CrossRefGoogle Scholar
  23. Shah, Y., Klessig, D.F. 1999. Salicylic acid: signal perception and transduction. In: Hooykaas, P.J.J., Hall, M.A., Libbenga, K.R. (eds), Biochemistry and Molecular Biology of Plant Hormones. Elsevier Science Publications. Amsterdam, The Netherlands. pp. 513–541.CrossRefGoogle Scholar
  24. Shetty, K. 1997. Biotechnology to harness the benefits of dietary phenolics; focus on Lamiaceae.Asia Pacific. J. Clin. Nutr. 6:162–171.Google Scholar
  25. Shetty, K., Randhir, R. 2005. Developmental stimulation of total phenolics and related antioxidant activity in light and dark germinated corn by natural elicitors. Process Biochem. 40:1721–1732.CrossRefGoogle Scholar
  26. Tabatabaei, S.A. 2014. The effect of priming on germination indexes and seed reserve utilization of maize seeds under salinity stress. J. Seed Sci. Technol. 3:44–51.Google Scholar
  27. Ventura, L., Dona, M., Macovei, A., Carbonera, D., Buttafava, A., Mondoni, A., Rossi, G., Balestrazzi, A. 2012. Understanding the molecular pathways associated with seed vigour. Plant Physiol. Biochem. 60:196–206.CrossRefGoogle Scholar
  28. Verstraeten, S.V., Keen, C.L., Schmitz, H.H., Fraga, C.G., Oteiza, P.L. 2003. Flavanols and procyanidins protect liposomes against lipid oxidation and disruption of the bilayer structure. Free Radic. Biol. Med. 34:84–92.CrossRefGoogle Scholar
  29. Waseem, M., Athar, H.U.R., Ashraf, M. 2006. Effect of salicylic acid applied through rooting medium on drought tolerance of wheat. Pakistan J. Bot. 38:1127–1136.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2017

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of ScienceDokuz Eylul UniversityBucaTurkey

Personalised recommendations