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Cereal Research Communications

, Volume 44, Issue 2, pp 309–319 | Cite as

Molecular Cloning and Identification of Novel ω-gliadin Genes from Triticum Species

  • Z. L. Li
  • D. D. Wu
  • H. Y. Li
  • G. Chen
  • W. G. Cao
  • S. Z. Ning
  • D. C. Liu
  • L. Q. ZhangEmail author
Quality and Utilization

Abstract

Gliadin is a main component of gluten proteins that affect functional properties of bread making and contributes to the viscous nature of doughs. In this study, thirteen novel ω-gliadin genes were identified in several Triticum species, which encode the ARH-, ATD-and ATN-type proteins. Two novel types of ω-gliadins: ATD- and ATN- have not yet been reported. The lengths of 13 sequences were ranged from 927 to 1269 bp and the deduced mature proteins were varied from 309 to 414 residues. All 13 genes were pseudogenes because of the presence of internal stop codons. The primary structure of these ω-gliadin genes included a signal peptide, a conserved N-terminal domain, a repetitive domain and a conserved C-terminus. In this paper, we first characterize ω-gliadin genes from T. timopheevi ssp. timopheevi and T. timopheevi ssp. araraticum. The ω-gliadin gene variation and the evolutionary relationship of ω-gliadin family genes were also discussed.

Keywords

ω-gliadin Triticum cloning pseudogene 

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Notes

Acknowledgements

This research was supported by the National Natural Science Foundation of China (31271723), Sichuan Provincial Youth Fund (2011JQ0016), and the Scientific Research Foundation of the Education Department of Sichuan Province (14ZA0012).

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Molecular Cloning and Identification of Novel ω-gliadin Genes from Triticum Species

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© Akadémiai Kiadó, Budapest 2016

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Z. L. Li
    • 1
  • D. D. Wu
    • 1
  • H. Y. Li
    • 1
  • G. Chen
    • 1
  • W. G. Cao
    • 2
  • S. Z. Ning
    • 1
  • D. C. Liu
    • 1
  • L. Q. Zhang
    • 1
    Email author
  1. 1.Triticeae Research InstituteSichuan Agricultural UniversityWenjiang, ChengduChina
  2. 2.Eastern Cereal and Oilseeds Research CentreAgriculture and Agri-Food CanadaOntarioCanada

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