Characterization of a New Wheat-Aegilops biuncialis 1Mb(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit
In this study, a new substitution line, 12-5-1, with 42 chromosomes that was derived from BC3F2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. The 12-5-1 was immune to both powdery mildew and stripe rust and has stable fertility. Multi-color fluorescence in situ hybridization indicated that 12-5-1 was a substitution line 1Mb(1B). The seed storage protein electrophoresis showed that 12-5-1 presented high molecular weight glutenin subunits (2+12) of CN19 and a new subunit designated as M which apparently originated from parent Ae. biuncialis, and absent 7+8 subunits. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness and mixing time of 12-5-1 were signifiantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1Mb-specifi polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1026, TNAC1041, TNAC1-02 and TNAC1-04, were also obtained. The new substitution line 1Mb(1B) 12-5-1 could be a valuable source for wheat improvement, especially for wheat end product quality and resistance to disease.
Keywordswheat Aegilops biuncialis bread-making quality HMW-GS multi-colour FISH
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This research was supported by the Applied Fundamental Research Fund of Sichuan Province (No. 2014JY0006), the National Natural Science Foundation of China (No. 31271420, No. 31330017, No. 31371682 and No. 31201203), and the National Transgenic Major Project (No. 2014ZX0801003B-002). We are grateful to Prof. Dr. Zongxiang Tang and Prof. Dr. Shulan Fu (State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Sichuan, China) for help and guidance of FISH analysis.
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