Transcriptome Profile of Early Responsive Genes in Susceptible Barley during Rhynchosporium secalis Infection
Scald caused by Rhynchosporium secalis, is an economically important disease found worldwide. In order to profile genes and pathways responding to R. seclais infection, leaf transcriptomes before and after fungus inoculation in susceptible barley were compared using cDNA-AFLP technique. Transcriptional changes of 144 expressed sequence tags (ESTs) were observed, of which 18 have no previously described function. Functional annotation of the transcripts revealed a wide range of pathways including cell wall fortification, cytoskeleton construction and metabolic processes at different time points. Furthermore, the results of RT-PCR analysis on candidate genes, ABC transporters and lycine-specific demethylase were consistent with the cDNA-AFLP data in their expression patterns. Taken together, our data suggest that susceptible barley reprograms metabolic and biological processes to initiate a suitable response R. secalis infection.
Keywordsbarley Rhynchosporium secalis defense response RT-PCR transcriptomics
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The authors thank the Director General of AECS and the Head of Biotechnology Department for their help throughout the period of this research.
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