Cereal Research Communications

, Volume 43, Issue 4, pp 537–543 | Cite as

Differently Expressed ‘Early’ Flavonoid Synthesis Genes in Wheat Seedlings Become to Be Co-regulated under Salinity Stress

  • O. Y. ShoevaEmail author
  • E. K. Khlestkina


Synthesis of flavonoid compounds in plants is associated with their response to environmental stress; however, the way in which the transcription of the relevant structural genes is regulated in stressed plants is still obscure. Transcription of the ‘early’ flavonoid synthesis genes Chi-1 and F3h-1 in the wheat coleoptile was investigated by quantitative real-time PCR in seedlings exposed to 100 mM or 200 mM NaCl. Under mild stress, transcript abundance of both Chi-1 and F3h-1 was increased significantly after six days of exposure. Under severe stress, the level of transcription was the same or even lower than that seen in nonstressed seedlings. In non-stressed conditions, the transcription patterns of Chi-1 and F3h-1 were quite distinct from one another, whereas under stress they became similar. An observed alteration in structural genes regulation mode under stress conditions may optimize flavonoid biosynthesis pathway to produce protective compounds with maximum efficiency.


salinity tolerance Triticum aestivum L. transcript abundance qRT-PCR 


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We thank Dr Robert Koebner ( for linguistic assistance during the preparation of the manuscript. This study was partially supported by RFBR (grant No 14-04-31637), a grant from the President of the Russian Federation (MD-2615.2013.4), and the State Budget Programme (Project No VI.53.1.5.).


  1. Borghesi, E., González-Miret, M.L., Escudero-Gilete, M.L., Malorgio, F., Heredia, F.J., Meléndez-Martínez, A.J. 2011. Effects of salinity stress on carotenoids, anthocyanins, and color of diverse tomato genotypes. J. Agric. Food Chem. 59:11676–11682.CrossRefGoogle Scholar
  2. Christie, P.J., Alfenito, M.R., Walbot, V. 1994. Impact of low-temperature stress on general phenylpropanoid and anthocyanin pathways – enhancement of transcript abundance and anthocyanin pigmentation in maize seedlings. Planta 194:541–549.CrossRefGoogle Scholar
  3. Das, P.K., Shin, D.H., Choi, S.-B., Park, Y.-I. 2012. Sugar-hormone cross-talk in anthocyanin biosynthesis. Mol. Cells 34:501–507.CrossRefGoogle Scholar
  4. Giovanini, M.P., Puthoff, D.P., Nemacheck, J.A., Mittapalli, O., Saltzmann, K.D., Ohm, H.W., Shukle, R.H., Williams C.E. 2006. Gene-for-gene defense of wheat against the Hessian fly lacks a classical oxidative burst. Mol. Plant-Microbe Interact. 19:1023–1033.CrossRefGoogle Scholar
  5. Himi, E., Maekawa, M., Noda, K. 2011. Differential expression of three flavanone 3-hydroxylase (F3H) genes in grains and coleoptiles of wheat. Int. J. Plant Genomic ID:369460.Google Scholar
  6. Hirayama, T., Shinozaki K. 2010. Research on plant abiotic stress responses in the post-genome era: past, present and future. Plant J. 61:1041–1052.CrossRefGoogle Scholar
  7. Ithal, N., Reddy, A.R. 2004. Rice flavonoid pathway genes, OsDfr and OsAns, are induced by dehydration, high salt and ABA, and contain stress responsive promoter elements that interact with the transcription activator, OsC1-MYB. Plant Sci. 166:1505–1513.CrossRefGoogle Scholar
  8. Khlestkina, E.K. 2013. The adaptive role of flavonoids: emphasis on cereals. Cereal Res. Commun. 41:185–198.CrossRefGoogle Scholar
  9. Khlestkina, E.K., Dobrovolskaya, O.B., Leonova, I.N., Salina, E.A. 2013. Diversification of the duplicated F3h genes in Triticeae. J. Mol. Evol. 76:261–266.CrossRefGoogle Scholar
  10. Khlestkina, E.K., Röder, M.S., Salina, E.A. 2008. Relationship between homoeologous regulatory and structural genes in allopolyploid genome – a case study in bread wheat. BMC Plant Biol. 8:88.CrossRefGoogle Scholar
  11. Khlestkina, E.K., Röder, M.S., Pshenichnikova, T.A., Börner, A. 2010. Functional diversity at the Rc (red coleoptile) gene in bread wheat. Mol. Breeding 25:125–132.CrossRefGoogle Scholar
  12. Lo Piero, A.R., Puglisi, I., Rapisarda, P., Petrone, G. 2005. Anthocyanins accumulation and related gene expression in red orange fruit induced by low temperature storage. J. Agric. Food Chem. 53:9083–9088.CrossRefGoogle Scholar
  13. Ma, D., Sun, D., Wang, C., Li, Y., Guo, T. 2014. Expression of flavonoid biosynthesis genes and accumulation of flavonoid in wheat leaves in response to drought stress. Plant Physiol. Biochem. 80:60–66.CrossRefGoogle Scholar
  14. Martin, C., Prescott, A., MacKay, S., Bartlett, J., Vrijlandt, E. 1991. Control of anthocyanin biosynthesis in flowers of Antirrhinum majus. Plant J. 1:37–49.CrossRefGoogle Scholar
  15. Offerman, J.D., Rychlik, W. 2003. Oligo primer analysis software. In: Krawetz, S.A., Womble D.D. (eds), Introduction to Bioinformatics: a Theoretical and Practical Approach. Humana Press. New Jersey, USA. pp. 345–361.Google Scholar
  16. Olenichenko, N.A., Ossipov, V.I., Zagoskina, N.V. 2006. Effect of cold hardening on the phenolic complex of winter wheat leaves. Russ. J. Plant Physiol. 53:495–500.CrossRefGoogle Scholar
  17. Olenichenko, N.A., Zagoskina, N.V., Astakhova, N.V., Trunova, T.I., Kuznetsov, Yu.V. 2008. Primary and secondary metabolism of winter wheat under cold hardening and treatment with antioxidants. Appl. Biochem. Microbiol. 44:535–540.CrossRefGoogle Scholar
  18. Quattrocchio, F., Wing, J.F., Leppen, H.T.C., Mol, J.N.M., Koes R.E. 1993. Regulatory genes controlling anthocyanin pigmentation are functionally conserved among plant species and have distinct sets of target genes. Plant Cell 5:1497–1512.CrossRefGoogle Scholar
  19. Shen, X. Y., Martens, S., Chen, M.L., Li, D.F., Dong, J.L., Wang, T. 2010. Cloning and characterization of a functional flavanone-3 beta-hydroxylase gene from Medicago truncatula. Mol. Biol. Rep. 37:3283–3289.CrossRefGoogle Scholar
  20. Shoeva, O.Y., Khlestkina, E.K. 2013. F3h gene expression in various organs of wheat. Mol. Biol. 47:901–903.CrossRefGoogle Scholar
  21. Shoeva, O.Y., Khlestkina, E.K., Berges, H., Salina, E.A. 2014. The homoeologous genes encoding chalcone–flavanone isomerase in Triticum aestivum L.: structural characterization and expression in different parts of wheat plant. Gene 538:334–341.CrossRefGoogle Scholar
  22. Tereshchenko, O.Y., Arbuzova, V.S., Khlestkina, E.K. 2013. Allelic state of the genes conferring purple pigmentation in different wheat organs predetermines transcriptional activity of the anthocyanin biosynthesis structural genes. J. Cereal Sci. 57:10–13.CrossRefGoogle Scholar
  23. Tereshchenko, O.Y., Khlestkina, E.K., Gordeeva, E.I., Arbuzova, V.S., Salina, E.A. 2012. Relationship between anthocyanin biosynthesis and abiotic stress in wheat. In: Börner, A., Kobijlski, B. (eds), Proceedings of the 15th International EWAC Conference, 2011, Novi Sad, Serbia. pp. 72–75.Google Scholar
  24. Treutter, D. 2006. Significance of flavonoids in plant resistance: a review. Environ. Chem. Lett. 4:147–157.CrossRefGoogle Scholar
  25. Van Oosten, M.J., Sharkhuu, A., Batelli, G., Bressan, R.A., Maggio, A. 2013. The Arabidopsis thaliana mutant air1 implicates SOS3 in the regulation of anthocyanins under salt stress. Plant Mol. Biol. 83:405–415.CrossRefGoogle Scholar
  26. Walia, H., Wilson, C., Condamine, P., Liu, X., Ismail, A.M., Zeng, L., Wanamaker, S.I., Mandal, J., Xu, J., Cui, X.P., Close, T.J. 2005. Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage. Plant Physiol. 139:822–835.CrossRefGoogle Scholar
  27. Winkel-Shirley, B. 2001. It takes garden. How work on diverse plant species has contributed to an understanding of flavonoid metabolism. Plant Physiol. 127:1399–1404.Google Scholar
  28. Zamora, P., Pardo, A., Fierro, A., Prieto, H., Zuniga, G.E. 2013. Molecular characterization of the chalcone isomerase gene family in Deschampsia antarctica. Polar. Biol. 36:1269–1280.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2015

Authors and Affiliations

  1. 1.Institute of Cytology and GeneticsSiberian Branch of the Russian Academy of SciencesNovosibirskRussia
  2. 2.Novosibirsk State UniversityNovosibirskRussia

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