Using Markers and Field Evaluation to Identify the Source of Eyespot Resistance Gene Pch1 in the Collection of Wheat Breeding Lines

Abstract

Pch1 gene translocated from Aegilops ventricosa provides effective resistance to eyespot in wheat. To track the Pch1 gene introgression, we investigated 372 genotypes obtained from various breeding programs using endopeptidase EpD1b marker, sequence-tagged-site (STS) marker XustSSR2001-7DL, and the score of infection index (K-index) evaluated after in vivo inoculation test. These genotypes were divided into three groups with 136, 124 and 112 genotypes for the field test lasting three years. In 2011, the mean K-index was 0.81, while 2012 and 2013 the mean K-indexes were 1.60 and 1.46, respectively. Both marker results indicated that 18 genotypes possessed Pch1 gene. Statistical analysis of the level of K-index showed that these 18 genotypes were resistant to eyespot, which verified the proper assignment of wheat genotypes with Pch1 gene based on the marker data. Thus, the endopeptidase and XustSSR2001-7DL are useful for identifying sources of eyespot resistance gene Pch1 in wheat breeding program.

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Acknowledgement

This work was financed by the Ministry of Agronomy and Rural Development (HOR hn - 801 - 11/13).

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Correspondence to M. Kwiatek.

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Authors’ Contribution and Conflict of Interest

M. Kwiatek and H. Wiśniewska participated equally in the research. H. Wiśniewska initiated the project and designed the study. M. Kwiatek, M. Majka, M. Gawłowska, K. Pankiewicz and J. Belter performed the markers analysis. M. Korbas and J. Danielewicz carried out the in vivo tests. Z. Kaczmarek made the statistical analysis. M. Kwiatek wrote the paper. The authors declare that they have no conflict of interest.

Communicated by X.F. Zhang

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Kwiatek, M., Wiśniewska, H., Kaczmarek, Z. et al. Using Markers and Field Evaluation to Identify the Source of Eyespot Resistance Gene Pch1 in the Collection of Wheat Breeding Lines. CEREAL RESEARCH COMMUNICATIONS 43, 638–648 (2015). https://doi.org/10.1556/0806.43.2015.024

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Keywords

  • endopeptidase
  • eyespot disease
  • inoculation tests
  • isozyme
  • STS marker
  • Triticum aestivum