Mapping QTL for Resistance against Pyrenophora tritici-repentis in Wheat

Abstract

Tan spot, caused by the fungus Pyrenophora tritici-repentis (Died.) Drechs is an important foliar disease of wheat (Triticum aestivum L.). From a set of phenotypically and molecularly characterized set of Argentinean isolates, two isolates H0019 and H0120 which do not correspond to known races of the pathogen were selected. Segregation for resistance among a set of recombinant inbred lines bred from the cross ‘W7984’ × cv. ‘Opata 85’ was used to identify the basis for resistance at the seedling stage, against those fungal isolates (H0019 and H0120), across three independent environments. On the basis of the mean performance across all three environments, a QTL against chlorosis located on the 6AS and linked to the RFLP locus Xksuh4c was significant for both isolates (with a LOD of 3.76 for isolate H0019 and 5.87 for H0120).

Abbreviations

PCR:

polymerase chain reaction

RILS:

recombinant inbreed lines

GS:

growth stage

LOD:

log likelihood ratio

References

  1. Ali, S., Francl. L.J. 2003. Population race structure of Pyrenophora tritici-repentis prevalent on wheat and noncereal grasses in the great plains. Plant Dis. 87:418–422.

    Article  PubMed  Google Scholar 

  2. Ali, S., Gurung, S., Adhikari, T.B. 2010. Identification and characterization of novel isolates of Pyrenophora tritici-repentis from Arkansas. Plant Dis. 94:229–235.

    Article  CAS  PubMed  Google Scholar 

  3. Anderson, J.A., Effertz, R.J. Faris, J.D., Francl, L.J., Meinhardt, S.W., Gill, B.S. 1999. Genetic analysis of sensitivity to Pyrenophora tritici-repentis necrosis inducing toxin in durum and common wheat. Phytopathol. 89:293–297.

    Article  CAS  Google Scholar 

  4. Andrie, R.M., Pandelova, I., Ciuffetti, L.M. 2007. A combination of phenotypic and genotypic characterization strengthens Pyrenophora tritici-repentis race identification. Phytopathol. 97:694–701

    Article  CAS  Google Scholar 

  5. Arseniuk, E., Czembor, P.C., Czaplicki, A., Song, Q., Cregan, P.B., Hoffman, D.L., Ueng, P.P. 2004. QTL controlling partial resistance to Stagonospora nodorum leaf blotch in winter wheat cultivar Alba. Euphytica 137:225–231.

    Article  CAS  Google Scholar 

  6. Bockus, W.W., Su, Z., Garret, K.A. Gill, B.S., Stack, J., Fritz, A.K., Roozeboom, K.L., Martin, T.J. 2007. Number of experiments needed to determine wheat disease phenotypes for four wheat diseases. Plant Dis. 91:103–108.

    Article  PubMed  Google Scholar 

  7. Effertz, R.J., Anderson J.A., Francl, L.J. 2001. Restriction fragment length polymorphism mapping of resistance to two races of Pyrenophora tritici-repentis in adult and seedling wheat. Phytopathol. 91:572–578.

    Article  CAS  Google Scholar 

  8. Effertz, R.J., Meinhardt, J.A., Anderson, J.A, Jordahl, J.G., Francl, L.J. 2002. Identification of a chlorosis-inducing toxin from Pyrenophora tritici-repentis and the chromosomal location of an insensitivity locus in wheat. Phytopathol. 92:527–533.

    Article  CAS  Google Scholar 

  9. Faris, J.D., Anderson, J.A.,Francl, L.J., Jordahl, J.G. 1996. Chromosomal location of a gene conditioning insensitivity in wheat to a necrosis-inducing culture filtrate from Pyrenophora tritici-repentis in wheat. Phytopathol. 86:459–463

    Article  CAS  Google Scholar 

  10. Faris, J.D., Anderson, J.A., Francl, L.J., Jordahl, J.G. 1997. RFLP mapping of resistance to chlorosis induction by Pyrenophora tritici-repentis in wheat. Theor. Appl. Genet. 94:98–103.

    Article  CAS  PubMed  Google Scholar 

  11. Faris, J.D. Liu, Z., Xu, S. 2013. Genetics of tan spot resistance. Theor. Appl. Genet. 126:2197–2217.

    Article  CAS  PubMed  Google Scholar 

  12. Ferreira, M.E., Grattapaglia, D. 1998. Introdução ao Uso de Marcadores Moleculares em Análise Genética (Introduction to the use of molecular markers in genetic analysis). 3ª ediçã. M.A. Embrapa, Brasilia. 220 p. (in Portuguese)

  13. Friesen, T.L., Faris, J.D. 2004. Molecular mapping of resistance to Pyrenophora tritici-repentis race 5 and sensitivity to Ptr Tox B in wheat. Theor. Appl. Genet. 109:464–471.

    Article  CAS  PubMed  Google Scholar 

  14. Gamba, F.M., Lamari, L., Brule-Babel, A. 1998. Inheritance of race-specific necrotic and chlorotic reactions induced by Pyrenophora tritici-repentis in hexaploid wheats. Can. J. Plant Pathol. 20:401–407.

    Article  Google Scholar 

  15. Ganal, M.W, Röder. M.S. 2007. Microsatellite and SNP markers in wheat breeding. In: Varshney, R.K., Tuberosa, R. (eds.), Genomics Assisted Crop Improvement: Vol. 2: Genomics Applications in Crops. Springer. Dordrecht, The Netherlands. pp. 1–24.

    Google Scholar 

  16. Genstat 2008. 3rd edition. VSN International, Hemel Hempstead, UK.

  17. Gurung, S., Mamidi, S., Bonman, J.M. Jackson, E.W., del Rio, L.E., Acevedo, M. Mergoum, M., Adhikari, T.B. 2011. Identification of novel genomic regions associated with resistance to Pyrenophora tritici-repentis races 1 and 5 in spring wheat landraces using association analysis. Theor. Appl. Gen. 123:1029–1041. Hosford, R.M. Jr. 1971. A form of Pyrenophora tricosthoma pathogenic to wheat and other grasses. Phytopathol. 61:28–32.

    Google Scholar 

  18. Hosford, R.M. Jr. 1982. Tan spot developing knowledge 1902–1981. In: Tan Spot of Wheat and Related Diseases Workshop. Horsford, R.M., Jr. (ed.), North Dakota State University. Fargo, USA. pp. 1–5.

    Google Scholar 

  19. Hosford, R.M. Jr., Busch, R.H. 1974. Losses in wheat caused by Pyrenophora trichostoma and Leptosphaeria avenaria f. sp. triticea. Phytopathol. 64:184–187.

    Google Scholar 

  20. Kohli, M., Mehta, Y., Ackerman de, M.D. 1992. Spread of tan spot in the Southern Cone Region of South America. In: Francl, L., Krupinsky, J., Mc Mullen, M. (eds), Advances in Tan Spot. Proc. Second Int. Tan Spot Workshop North Dakota State University. Fargo, ND, USA. pp. 86–90.

    Google Scholar 

  21. Lamari, L., Bernier, C.C. 1989a. Toxin of Pyrenophora tritici-repentis: Host specificity, significance in disease, and inheritance of host reaction. Phytopathol. 79:740–744.

    Article  CAS  Google Scholar 

  22. Lamari, L., Bernier, C.C. 1989b. Virulence of isolates of Pyrenophora tritici-repentis on 11 wheat cultivars and cytology of the differential host reaction. Can. J. Plant Pathol. 11:284–290

    Article  Google Scholar 

  23. Lamari, L., Bernier, C.C. 1991. Genetics of tan necrosis and extensive chlorosis in tan spot of wheat caused by Pyrenophora tritici-repentis. Phytopathol. 81:1092–1095.

    Article  Google Scholar 

  24. Lamari, L., Strelkov, S.E. Yahyaoui, A., Orabi, J., Smith, R.B. 2003. The identification of two new races of Pyrenophora tritici-repentis from the host centre of diversity confirms a one-to one relationship in tan spot of wheat. Phytopathol. 93:391–396.

    Article  CAS  Google Scholar 

  25. Lepoint, P., Renard, E. Legreve, E. Duveiller, E., Maraite, H. 2010. Genetic diversity of the mating type and toxin production genes in Pyrenophora tritici-repentis. Phytopathol. 100:474–483.

    Article  CAS  Google Scholar 

  26. Martinez, J.P., Oesch, N.W., Ciuffetti, L.M. 2004. Characterization of the multiple- copy host-selective toxin gene, ToxB, in pathogenic and nonpathogenic isolates of Pyrenophora tritici-repentis. Mol. Plant Microbe Interactions 17:467–474.

    Article  CAS  Google Scholar 

  27. Moreno, M.V., Stenglein, S., Perello, A.E., Balatti, P. 2008. Pathogenic and genetic diversity of isolates of Pyrenophora tritici-repentis causing tan spot of wheat in Argentina. Eur. J. Plant Pathol. 122:239–252.

    Article  Google Scholar 

  28. Moreno, M.V., Stenglein, S., Perelló, A.E. 2015. Distribution of races and Tox genes in Pyrenophora tritici-repentis isolates from wheat in Argentina. Tropical Plant Pathol, 40:141–146.

    Article  Google Scholar 

  29. Nelson, J.C. 1997. QGene: Software for mapping-based genomic analysis and breeding. Mol. Breed. 3:239–245.

    Article  CAS  Google Scholar 

  30. Noriel, A.J., Sun, X., Bockus, W., Bai, G. 2011. Resistance to tan spot and insensitivity to Ptr ToxA in wheat. Crop Sci. 51:1059–1067

    Article  Google Scholar 

  31. Odvody, G., Boosalis, M.G.. 1982. An efficient conidial production technique for Pyrenophora trichostoma. In: Tan Spot of Wheat and Related Disease Workshop. Hosford, R. M., Jr. (ed.), North Dakota State University, Fargo, USA. 116 p.

    Google Scholar 

  32. Orolaza. N.P., Lamari, L., Balance, G.M. 1995. Evidence of a host-specific chlorosis toxin from Pyrenophora tritici-repentis, the causal agent of tan spot of wheat. Phytopathol. 85:1282–1287.

    Article  CAS  Google Scholar 

  33. Patel, J.S., Mamidi, S., Michael Bonman, J., Adhikari, T.B. 2013. Identification of QTL in spring wheat associated with resistance to a novel isolate of Pyrenophora tritici-repentis. Crop Sci. 53:453–483.

    Article  CAS  Google Scholar 

  34. Perelló, A.E., Moreno, M.V., Simón, M.R., Sisterna, M. 2003. Tan spot of wheat infection at different stage of crop development and inoculums type. Crop Prot. 22:157–169.

    Article  Google Scholar 

  35. Platt, H., Morrall, R.A., Gruen, H.E. 1977. The effects of substrate, temperature and photoperiod on conidiation of Phyrenophora tritici-repentis. Can. J. Bot. 55:254:259.

    Article  Google Scholar 

  36. Rees, R.G., Mayer, R.J., Platz, G.J. 1981. Yield losses in wheat from yellow spot: A disease-loss relationship derivated from single tillers. Aust. J. Agr. Res. 32:851–859.

    Article  Google Scholar 

  37. Rees, R.G., Platz, G.J. 1983. Effects of yellow spot on wheat: Comparison of epidemics at different stages of crop development. Aust. J. Agric. Res. 4:39–46.

    Article  Google Scholar 

  38. Simón, M.R., Ayala, F., Terrile, I., Golik, S., Perelló, A., Cordo, C., Chidichimo, H. 2011. Integrated foliar disease management to prevent yield loss in Argentinian wheat production. Agron. J. 103:1441–1451.

    Article  Google Scholar 

  39. Singh, P.K., Gonzalez-Hernandez, J.L., Mergoum, M., Ali, S., Adhikari, T.B., Kianian, S.F., Elias, E.M., Hughes, G.R. 2006. Identification and molecular mapping of a gene conferring resistance to Pyrenophora tritici-repentis race 3 in tetraploid wheat. Phytopathol. 96:885–889.

    Article  CAS  Google Scholar 

  40. Singh, P.K., Mergoum, M., Gonzalez-Hernandez, J.L., Ali, S., Adhikari, T.B., Kianian, S.F., Elias, E.M. Hughes, G.R. 2008. Genetics and molecular mapping of resistance to necrosis inducing race 5 of Pyrenophora tritici-repentis in tetraploid wheat. Mol. Breeding 21:293–304.

    Article  CAS  Google Scholar 

  41. Stenglein, S.A., Balatti, P.A. 2006. Genetic diversity of Phaeoisariopsis griseola in Argentina as revealed by pathogenic and molecular markers. Physiol. and Mol. Plant Pathol. 68:158–167.

    Article  CAS  Google Scholar 

  42. Strelkov, S.E., Lamari, L. 2003 Host-parasite interaction in tan spot Pyrenophora tritici-repentis of wheat. Can. J. Plant Pathol. 25:339–349.

    Article  CAS  Google Scholar 

  43. Strelkov, S.E., Kowatsch, R.F., Balance, G.M., Lamari, L. 2006. Characterization of the ToxB gene from North America and Canadian isolates of Pyrenophora tritici-repentis. Physiol. and Mol. Plant Pathol. 67:164–170.

    Article  CAS  Google Scholar 

  44. Sun,X.C., Bockus, W., Bai, G.H. 2010. Quantitative trait loci for resistance to Pyrenophora tritici-repentis race 1 in Chinese wheat. Phytopathol. 100:468–473.

    Article  Google Scholar 

  45. Tadesse, W., Hsam, S.L.K., Wenzel, G., Zeller, F.J. 2006a. Identification and monosomic analysis of tan spot resistance and chromosomal location of a resistance gene in the cultivar Salamouni. Plant Breed. 125:318–322.

    Article  Google Scholar 

  46. Tadesse, W., Hsam, S.L.K., Zeller, F.J. 2006b. Evaluation of common wheat cultivars for tan spot (Pyrenophora tritici-repentis) in synthetic wheat lines. Theor. Appl. Genet. 14:885–862.

    Google Scholar 

  47. Tadesse, W., Schmolke, M., Mohler, V., Wenzel, G., Hsam, S.L.K., Zeller, F.J. 2007. Molecular mapping of resistance genes to tan spot (Pyrenophora tritici-repentis) in synthetic wheat lines. Theor. Appl. Genet. 14:885–862.

    Google Scholar 

  48. Van Deynze, A.E., Dubcovsky, J., Gill, K.S., Nelson, J.C., Sorrells, M.E., Dvořák, J., Gill, B.S., Lagudah, E.S., McCouch, S.R., Appels, R. 1995. Molecular-genetic maps for group 1 chromosomes of Triticeae species and their relation to chromosomes in rice and oat. Genome 38:45–49.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  49. Wiese, M.V. 1987. Tan spot (yellow leaf spot). In: Wiese, M.V. (ed.), Compendium of wheat diseases. The Am. Phytopathol. Soc., APS Press. St. Paul, MN, USA. pp. 42–43.

    Google Scholar 

  50. Zadoks, J.C., Chang, T.T., Konzak, C.F. 1974. A decimal code for the growth stages of cereals. Weed Res. 14:415–421.

    Article  Google Scholar 

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Correspondence to M. R. Simón.

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Communicated by X.F. Zhang

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Simón, M.R., Ayala, F.M., Moreno, M.V. et al. Mapping QTL for Resistance against Pyrenophora tritici-repentis in Wheat. CEREAL RESEARCH COMMUNICATIONS 43, 649–660 (2015). https://doi.org/10.1556/0806.43.2015.022

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Keywords

  • tan spot
  • resistance
  • races
  • QTL