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Cereal Research Communications

, Volume 43, Issue 4, pp 591–603 | Cite as

Differential and Temperature Dependent Regulation of ADP-Glucose Pyrophosphorylase by Specific Chromosome in Wheat Grains

  • N. Passricha
  • R. Batra
  • R. K. BehlEmail author
  • V. K. Sikka
Physiology

Abstract

A stock of disomic chromosome substitution (DCS) lines having specific chromosome of wheat variety C591 substituted in the background of rest of Chinese spring chromosomes, were used to analyze grain yield components as a function of enzyme activity of ADP—glucose pyrophosphorylase (AGPase), a starch biosynthesis enzyme in wheat grains. Associations between yield characteristics, grain growth rate (GGR) and AGPase enzyme activity of DCS lines suggested a major involvement of chromosome 3A, 4B, 7D and 2D in a temperature dependent manner. Assessment of AGPase assay at different developmental stages such as 14, 21, 28 days post anthesis (DPA) embodied that gene(s) for this enzyme are present on specific chromosomes and operate at different stages of grain development. The DCS line with 7D chromosome has a major contribution in determining the grain starch content. In this line, AGPase enzyme activity was highest at 21 DPA and was the most crucial determinant in its high GGR. Line 4B performed well at only early stage (14 DPA) suggesting that line 4B AGPase requires a lower temperature range for activation as compared to 7D line. Line 3A had substantially reduced (40%) test weights revealing the presence of few down-regulatory elements on chromosome 3A to reduce the activity of AGPase. The DCS line 2D showed higher test weights and grain number than all other lines ascribed to a consistent AGPase activity along with an efficient mechanism for translocation of photosynthates from source to sink. The chromosome 2D shows positive relation with yield attributes therefore, it can be employed to improve wheat productivity via analytical breeding programme.

Keywords

AGPase enzyme chromosome days post anthesis (DPA) days to anthesis (DTA) disomic substitution line (DCS) grain growth rate (GGR) wheat 

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Differential and Temperature Dependent Regulation of ADP-Glucose Pyrophosphorylase by Specific Chromosome in Wheat Grains

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© Akadémiai Kiadó, Budapest 2015

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • N. Passricha
    • 1
  • R. Batra
    • 1
  • R. K. Behl
    • 2
    Email author
  • V. K. Sikka
    • 1
  1. 1.Molecular Biology and Biotechnology, College of Basic SciencesCCS Haryana Agricultural UniversityHissarIndia
  2. 2.Genetics and Plant Breeding, College of Agricultural SciencesCCS Haryana Agricultural UniversityHissarIndia

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