Acta Biologica Hungarica

, Volume 69, Issue 4, pp 385–395 | Cite as

Inhibition of the Formation of Amyloid-Like Fibrils with Spices, Especially Cloves

  • Márta KotormánEmail author
  • Alexandra Varga
  • Phanindra Babu Kasi
  • János Nemcsók


During the study of inhibition of amyloid fibril formation, a-chymotrypsin protein was developed in 55% ethanol at pH 7.0. We investigated the inhibitory effect of different spices on amyloid fibril formation using turbidity measurements and Congo red binding assays. We found that all spices except the black pepper and caraway seed prevented fibril formation. The highest inhibition was measured with the clove, which reduced the amount of aggregates by 90%. We studied the inhibitory effect of the cloves at different concentrations on aggregation, it was found that the inhibitory activity of clove is dependent on concentration. We have measured the total phenolic content of the spice extracts too. Based on all these findings we have come to the following conclusion: Our results indicate that spices can contain other compounds too — not only phenolic compounds — which influence the formation of amyloid fibrils, and the effectiveness of various phenolic compounds are different.


α-chymotrypsin amyloid fibrils clove polyphenols spice 


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© Akadémiai Kiadó Zrt. 2018

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Márta Kotormán
    • 1
    Email author
  • Alexandra Varga
    • 1
  • Phanindra Babu Kasi
    • 1
    • 2
  • János Nemcsók
    • 3
  1. 1.Department of Biochemistry and Molecular Biology, Faculty of Science and InformaticsUniversity of SzegedSzegedHungary
  2. 2.Doctoral School in Biology, Faculty of Science and InformatiesUniversity of SzegedSzegedHungary
  3. 3.Department of Biology, Pedagogical FacultySelye János UniversityKomarnoSlovak Republic

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