ABA Regulation of Antioxidant Activity During Post-Germination Desiccation and Subsequent Rehydration in Wheat
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ABA regulation of antioxidant activity during post-germination desiccation and subsequent rehydration was studied in two wheat cultivars PBW 644 (ABA-higher sensitive and drought tolerant) and PBW 343 (ABA-lesser sensitive and drought susceptible) where 1 d-germinated seeds were exposed to ABA/ PEG- 6000 for next 1 d, desiccated for 4 d and subsequently rehydrated for 4 d. Ascorbate, dehydrascorbate to ascorbate ratio, malondialdehyde (MDA), hydroxyl radicals, and activities of monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), alcohol dehydrogenase (AlcDH) and aldehyde dehydrogenase (AldDH) were measured in seedlings just before desiccation (2 d old), desiccated (6 d old) and rehydrated (10 d old) stages. ROS/NO signaling was studied under CT and ABA supply by supplying ROS and NO scavengers. During desiccation, both cultivars showed increase of oxidative stress (dehydroascorbate to ascorbate ratio, MDA, hydroxyl radicals) and antioxidant activity in the form of ascorbate content and AldDH activity while other antioxidant enzymes were not increased. PBW 644 showed higher antioxidant activity thus produced less oxidative stress compared to PBW 343. During rehydration, activities of all antioxidant enzymes and levels of ROS (hydroxyl radicals) were increased in both cultivars and MDA was decreased in PBW 343. ABA supply improved desiccation as well as rehydration by improving all parameters of antioxidant activity tested in this study. PEG supply resembled to ABA-supply for its effects. ABA/PEG improvements were seen higher in PBW 644. ROS/NO-signalling was involved under CT as well as under ABA for increasing antioxidant activity during desiccation as well as rehydration in both cultivars.
Key wordsAbscissic acid antioxidant nitric oxide post-germination desiccation reactive oxygen species
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- 1.Arakawa, N., Tsutsumi, K., Sanceda, N. G., Kurata, T., Inagaki, C. (1981) A rapid and sensitive method for the determination of ascorbic acid using 4,7-Diphenyl-l,10-phenanthroline. Agric. Biol. Chem. 45, 1289–1290.Google Scholar
- 4.Dinakar, C., Bartels, D. (2013) Desiccation tolerance in resurrection plants: new insights from transcriptome, proteome, and metabolome analysis. Front. Plant Sci. 4, e482.Google Scholar
- 7.Gaff, D. F., Oliver, M. (2013) The evolution of desiccation tolerance in angiosperm plants: a rare yet common phenomenon. Funct. Plant Biol. 40, 315–328.Google Scholar
- 8.Gechev, T. S., Benina, M., Obata, T., Tohge, T., Sujeeth, N., Minkov, I., Hille, J., Temanni, M.-R., Marriott, A. S., Bergstrom, E., Thomas-Oates J., Antonio, C., Mueller-Roeber, B., Schippers, J. H. M., Fernie, A. R., Toneva, V. (2013) Molecular mechanisms of desiccation tolerance in the resurrection glacial relic Haberlea rhodopensis. Cell. Mol. Life Sci. 70, 689–709.PubMedGoogle Scholar
- 9.Gill, S. S., Tuteja, N. (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48, 909–930.Google Scholar
- 11.Heath, R. L., Packer, L. (1968) Photoperoxidation in isolated chloroplasts. I. kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125, 180–198.Google Scholar
- 12.Hossain, M. A., Bhattacharjee, S., Armin, S.-M., Qian, P., Xin, W., Li, H.-Y., Burritt, D. J., Fujita, M., Tran, L.-SP. (2015) Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging. Front. Plant Sci. 6, e420.Google Scholar
- 16.Kaur, L., Gupta, A. K., Zhawar, V. K. (2014) ABA improvement of antioxidant metabolism under water stress in two wheat cultivars contrasting in drought tolerance. Indian J. Plant Physiol. 19, 189–196.Google Scholar
- 18.Kerchev, P. I., Pellny, T. K., Vivancos, P. D., Kiddle, G., Hedden, P., Driscoll, S., Vanacker, H., Verrier, P., Hancock, R. D., Foyer, C. H. (2011) The transcription factor ABI4 is required for the ascorbic acid-dependent regulation of growth and regulation of jasmonate-dependent signaling pathways in Arabidopsis. Plant Cell 23, 3319–3334.PubMedPubMedCentralGoogle Scholar
- 20.Kour, S., Zhawar, V. K. (2018) ABA regulation of post-germination desiccation tolerance in wheat cultivars contrasting in drought tolerance. An. Acad. Bras. Cienc. (Accepted).Google Scholar
- 21.Kranner, I., Richard, P., Beckett, R. P., Wornik, S., Zorn, M., Pfeifhofer, H. W. (2002) Revival of a resurrection plant correlates with its antioxidant status. Plant J. 31, 13–24.Google Scholar
- 22.Leprince, O., Buitink, J. (2010) Introduction to desiccation biology: from old borders to new frontiers. Planta 242, 369–378.Google Scholar
- 32.Nedeva, D., Nikolova, A. (1997) Desiccation tolerance in developing seeds. Bulg. J. Plant Physiol. 23, 100–113.Google Scholar
- 33.Omoto, E., Nagao, H., Taniguchi, M., Miyake, H. (2013) Localization of reactive oxygen species and change of antioxidant capacities in mesophyll and bundle sheath chloroplasts of maize under salinity. Plant Physiol. 149, 1–12.Google Scholar
- 37.Sgherri, C., Stevanovic, B., Navari-Izzo, F. (2004) Role of phenolic acid during dehydration and rehydration of Ramonda serbica. Physiol. Plant. 122, 478–485.Google Scholar
- 40.Vieira, C. V., Amaral da Silva, E. A., de Alvarenga, A. A., de Castro, E. M., Toorop, P. E. (2010) Stress-associated factors increase after desiccation of germinated seeds of Tabebuia impetiginosa Mart. Plant Growth. Regul. 62, 257–263.Google Scholar
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