Acta Biologica Hungarica

, Volume 68, Issue 4, pp 428–442 | Cite as

Establishment of an Efficient Plant Regeneration Culture Protocol and Achievement of Successful Genetic Transformation in Jatropha curcas L.

  • Ying LiuEmail author
  • Guoxuan Liu
  • Yali Yang
  • Sufang Niu
  • Fuguang Yang
  • Shaoxia Yang
  • Jianian Tang
  • Jianping Chen


An efficient and reproducible protocol is described for shoot-bud regeneration and Agrobacterium tumefaciens-mediated genetic transformation of J. curcas. Treating the explants with high concentrations (5–120 mg/L) of TDZ for short durations (5–80 min) before inoculation culture increased significantly the regeneration frequency and improved the quality of the regenerated buds. The highest shoot-buds induction rate (87.35%) was achieved when petiole explants were treated with 20 mg/L TDZ solution for 20 min and inoculated on hormone-free MS medium for 30 days. Regenerated shoots of 0.5 cm or a little longer were isolated and grafted to seedling stocks of the same species, and then the grafted plantlets were planted on half-strength MS medium containing 0.1 mg/L IBA and 2 mg/L sodium nitroprusside (SNP). This grafting strategy was found to be very effective, to obtain that healthy grafted plantlets ready for acclimatization within 20 days. By the above mentioned protocol and with general Agrobacterium–mediated genetic transformation methods only 65 days were needed to obtain intact transgenic plants.


Jatropha curcas Petiole explants Plant regeneration Genetic transformation 


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© Akadémiai Kiadó, Budapest 2017

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Ying Liu
    • 1
    Email author
  • Guoxuan Liu
    • 1
  • Yali Yang
    • 2
  • Sufang Niu
    • 3
  • Fuguang Yang
    • 4
  • Shaoxia Yang
    • 1
  • Jianian Tang
    • 1
  • Jianping Chen
    • 5
  1. 1.Department of Biotechnology, Faculty of Agricultural ScienceGuang Dong Ocean UniversityZhanjiang, GuangdongP.R. China
  2. 2.Department of Basic Medicine, School of MedicineJiaying UniversityMeizhou, GuangdongP.R. China
  3. 3.Department of Marine Biology, Fisheries CollegeGuang Dong Ocean UniversityZhanjiang, GuangdongP.R. China
  4. 4.Faculty of Mathematics and Computer Science, College of Software EngineeringGuang Dong Ocean UniversityZhanjiang, GuangdongP.R. China
  5. 5.Department of Food Science and Engineering, College of Food Science and TechnologyGuang Dong Ocean UniversityZhanjiang, GuangdongP.R. China

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