Acta Biologica Hungarica

, Volume 68, Issue 4, pp 466–476 | Cite as

Chemical Composition and Antimicrobial Activity of the Commercial Origanum onites L. Oil Against Nosocomial Carbapenem Resistant Extended Spectrum Beta Lactamase Producer Escherichia coli Isolates

  • Banu KaskatepeEmail author
  • Serap Suzuk Yildiz
  • Merve Eylul Kiymaci
  • Ayse Nur Yazgan
  • Salih Cesur
  • Sinem Aslan Erdem


In recent years rapidly growing antibiotic resistance has increased interest toward natural products, especially essential oils because of their various effects. The aim of this study was to identify the chemical composition of the commercial Origanum onites essential oil (EO) and to investigate the antimicrobial activity by disc diffusion and dilution methods, against ten different ATCC strains, including eight bacteria, two yeasts and seventy-nine clinical nosocomial Escherichia coli isolates that produce extended spectrum beta lactamase (ESBL). The chemical composition of EO was analyzed by GC and GC-MS. The major compounds of the EO were determined as carvacrol (51.4%) followed by linalool (11.2%), p-cymene (8.9%) and γ-terpinene (6.7%). O. onites EO had antimicrobial activity against all standard strains and inhibited microbial growth of ESBL positive E. coli isolates. According to our results, O. onites EO may be an alternative to synthetic drug, used in combination with other antibiotics for treatment of infection caused by multidrug resistant bacteria after testing toxic effects and irritation at preferred doses on human.


Antimicrobial activity nosocomial ESBL (+) E. coli GC/MS Origanum onites essential 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Adams, R. P. (2001) Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy. Allured Publishing Co., Illinois, USA.Google Scholar
  2. 2.
    Akgül, A., Kıvanç, M. (1998) Inhibitory effects of selected Turkish spices and oregano components on some food borne fungi, Intern. J. Food. Microbiol. 6, 263–268.CrossRefGoogle Scholar
  3. 3.
    Askun, T., Tumen, G., Satil, F., Ates, M. (2009) Characterization of the phenolic composition and antimicrobial activities of Turkish medicinal plants. Pharm. Biol. 47, 563–571.CrossRefGoogle Scholar
  4. 4.
    Baser, K. H. C. (2004) Domestic uses of Oregano in Turkey. In: Kitzios, S. E. (ed.) Oregano: The genera Origanum and Lippia. Taylor & Francis, London, pp. 111.Google Scholar
  5. 5.
    Baser, K. H. C. (2008) Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Curr. Pharm. Design. 14, 106–3019.Google Scholar
  6. 6.
    Baydar, H., Sagdiç, O., Ozkan, G., Karadoğan, T. (2004) Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control. 15, 169–172.CrossRefGoogle Scholar
  7. 7.
    Beklem Bostancıoğlu, R., Kürkçüoğlu, M., Başer, K. H., Koparal, A. T. (2012) Assessment of antiangiogenic and anti-tumoral potentials of Origanum onites L. essential oil. Food Chem. Toxicol. 50, 2002–2008.CrossRefGoogle Scholar
  8. 8.
    Boyraz, N., Ozcan, M. (2006) Inhibition of phytopathogenic fungi by essential oil, hydrosol, ground material and extract of summer savory (Satureja hortensis L.) growing wild in Turkey. Int. J. Food Microbiol. 107, 238–42CrossRefGoogle Scholar
  9. 9.
    Cantón, R., Novais, A., Valverde, A., Machado, E., Peixe, L., Baquero, F. (2008) Prevalence and spread of extended-spectrum beta-lactamase-producing Enterobacteriaceae in Europe. Clin. Microbiol. Infect. 14, 144–153.CrossRefGoogle Scholar
  10. 10.
    CLSI (2013) Performance Standards for Antimicrobial Susceptibility Testing, Twenty-Third Informational Supplement, Wayne PA, M100-S23, Vol. 33, No.1, January.Google Scholar
  11. 11.
    Cosentino, S., Tuberoso, C. I., Pisano, B., Satta, M., Mascia, V., Arzedi, E., Palmas, F. (1999) Palmas, In-vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Lett. Appl. Microbiol. 29, 130–135.CrossRefGoogle Scholar
  12. 12.
    Demirci, F., Paper, D. H., Franz, G., Başer, K. H. C. (2004) Investigation of the Origanum onites L. essential oil using the chorioallantoic membrane assay. J. Agr. Food Chem. 52, 251–254.CrossRefGoogle Scholar
  13. 13.
    EUCAST (2016) Breakpoint tables for interpretation of MICs and zone diameters, Version 6.0. European Committee on Antimicrobial Susceptibility Testing (EUCAST); [cited 2016-01-01]. Avaiablefrom: Scholar
  14. 14.
    Fachini-Queiroz, F. C., Kummer, R., Estevão-Silva, C. F., Barros Carvalho, M. D., Cunha, M. J., Grespan, R., Bersani-Amado, C. A., Cuman, R. K. N. (2012) Effects of Thymol and Carvacrol, Constituents of Thymus vulgaris L. Essential Oil, on the Inflammatory Response. J Evid Based Complementary Altern Med. vol. 2012, Article ID 657026, 10 pages.Google Scholar
  15. 15.
    Holley, R. A., Patel, D. (2005) Improvement of shelflife and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Microbiol. 22, 273–292.CrossRefGoogle Scholar
  16. 16.
    Kacar, O., Göksu, E., Azkan, N. (2006) The effect of different plant densites on some agronomic and quality characteristics of Oregano (Origanum onites L.) The Journal of Agricultural Faculty of Uludag Universit. 2, 51–60.Google Scholar
  17. 17.
    Kan, Y., Uçan, U. S., Kartal, M., Altun, M. L., Aslan, S., Sayar, E., Ceyhan, T. (2006) GC-MS analysis and antibacterial activity of cultivated Satureja cuneifolia Ten essential oil. Turkish Journal of Chemistr. 30, 253–259.Google Scholar
  18. 18.
    Kintzios, S. E. (2002) Profile of thyme multifaceted prince of the herbs. In: Kitzios, S. E. (ed.) Oregano: The genera Origanum and Lippia. Taylor & Francis, London, 3–8.Google Scholar
  19. 19.
    Kirimer, N., Başer, K. H. C., Tümen, G. (1995) Carvacrol-rich plants in Turkey. Chem Nat Compd. 31, 37–41.CrossRefGoogle Scholar
  20. 20.
    Kordali, S., Cakir, A., Ozer, H., Cakmakci, R., Kesdek, M., Mete, E. (2008) Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresource Technol. 99, 8788–8795.CrossRefGoogle Scholar
  21. 21.
    Mith, H., Dure, R., Delcenserie, V., Zhiri, A., Daube, G., Clinquart, A. (2014) Antimicrobial activities of commercial essential oils and their components against food-borne pathogens and food spoilage bacteria. Food Sci Nutr. 2, 403–416.CrossRefGoogle Scholar
  22. 22.
    Şahin, N. F., Güllüce, M., Dafererad, D., Sökmen, A., Sökmen, M., Polissioud, M., Agar, G., Özer, H. (2004) Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. vulgare in the Eastern Anatolia region of Turkey. Food Contro. 15, 549–557.CrossRefGoogle Scholar
  23. 23.
    Sarac, N., Ugur, A. (2008) Antimicrobial activities of the essential oils of Origanum onites L., Origanum vulgare L. subspecies hirtum (link) letswaart, Satureja thymbra L., and Thymus cilicicus Boiss. & Bal. growing wild in Turkey. J Med Food. 11, 568–573.CrossRefGoogle Scholar
  24. 24.
    Sarikurkcu, C., Zengin, G., Oskay, M., Uysal, S., Ceylan, R., Aktumsek, R. (2015) Composition, antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp. vulgare and subsp. hirtum) essential oils. Ind. Crops Prod. 70, 178–184.CrossRefGoogle Scholar
  25. 25.
    Schulz, H., Özkan, G., Baranska, M., Krüger, H., Özcan, M. (2005) Characterization of essential oil plants from Turkey by IR and Raman spectroscopy. Vibrational Spectroscop. 39, 249–256.CrossRefGoogle Scholar
  26. 26.
    Sertkaya, E., Kaya, K. (2010) Acaricidal activities of the essential oils from several medicinal plants against the carmine spider mite (Tetranychus cinnabarinus Boisd.) (Acarina: Tetranychidae). Ind Crops Prod. 31, 107–112.CrossRefGoogle Scholar
  27. 27.
    Skandamis, P., Tsigarida, E., Nychas, G. J. E. (2002) The effect of oregano essential oil on survival/ death of Salmonella typhimurium in meat stored at 50 °C under aerobic, VP/MAP conditions. Food Microbiol. 19, 97–103.CrossRefGoogle Scholar
  28. 28.
    Solgi, M., Kafi, M., Taghavi, T., Naderi, R. (2009) Essential oils and silver nanoparticles (SNP) as novel agents to extend vase-life of gerbera (Gerbera jamesonii cv. ‘Dune’) flowers. Postharvest Biol. Technol. 53, 155–158.CrossRefGoogle Scholar
  29. 29.
    Solórzano-Santos, F., Miranda-Novales, M. G. (2012) Essential oils from aromatic herbs as antimicrobial agents. Curr. Opin Biotechnol. 23, 136–141.CrossRefGoogle Scholar
  30. 30.
    Tozlu, E., Cakir, A., Kordali, S., Tozlu, G., Ozer, H., Akcin, T. A. (2011) Chemical compositions and insecticidal effects of essential oils isolated from Achillea gypsicola, Satureja hortensis, Origanum acutidens and Hypericum scabrum against broadbean weevil (Bruchus dentipes). Sci. Horticult. 130, 9–17.CrossRefGoogle Scholar
  31. 31.
    Vázquez, B. I., Fente, C., Franco, C. M., Vázquez, M. J., Cepeda, A. (2001) Inhibitory effects of eugenol and thymol on Penicillium citrinum strains in culture media and cheese. Int. J. Food Microbiol. 67, 157–163.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2017

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Banu Kaskatepe
    • 1
    Email author
  • Serap Suzuk Yildiz
    • 2
  • Merve Eylul Kiymaci
    • 1
  • Ayse Nur Yazgan
    • 3
  • Salih Cesur
    • 4
  • Sinem Aslan Erdem
    • 3
  1. 1.Department of Pharmaceutical MicrobiologyAnkara UniversityAnkaraTurkey
  2. 2.National Antimicrobial Resistance LaboratoryPublic Health Institution of TurkeyAnkaraTurkey
  3. 3.Department of PharmacognosyAnkara UniversityAnkaraTurkey
  4. 4.Department of Infection DiseasesAnkara Training HospitalAnkaraTurkey

Personalised recommendations