Abstract
This study was aimed to evaluate the pharmacological potential of various extracts (hexane, chloroform, methanol and aqueous) of dried shoots of Triticum aestivum (wheat grass) in terms of antiproliferative and hepatoprotective potential of T. aestivum. The total chlorophyll content in dried shoots of T. aestivum was 0.54 ± 0.016 g/L (chlorophyll-a: 0.288 ± 0.05 g/L; and chlorophyll-b; 0.305 ± 0.05 g/L), while total carotene content was 0.42 ± 0.066 g/L. In addition, the chloroform extract of dried shoots of T. aestivum (250 µg/mL) exhibited 87.23% inhibitory effect with potent cytotoxicity against human hepatocellular carcinoma (HepG2) cancer cell line. Moreover, chloroform and methanol extracts significantly reduced the levels of SGOT, and SGPT enzymes, as well as total bilirubin content, while raised the level of total protein in a concentration-gradient manner, confirming the potent hepatoprotective effect of T. aestivum. A possible mechanism of apoptosis of the chloroform extract of dried shoots of T. aestivum in terms of its potent antiproliferative activity against HepG2 cancer cell line can also be proposed in this study. Our findings clearly demonstrate that T. aestivum has a significant pharmacological potential that night be used for antiproliferative and hepatoprotective purposes.
References
- 1.
Adams, J. V., Slaght, K. S., Boogaard, M. A. (2004) An automated approach to lithcfield and Wilcoxon’s evaluation of dose-effect experiments using the R package LW1949. Environ. Toxicol. Chem. 35, 3058–3061.
- 2.
Adewusi, E. A., Afolayan, A. J. (2010) A review of natural products with hepatoprotective activity. J. Med. Plant Res. 4, 1318–1334.
- 3.
Aneta, W., Jan, O. S., Renata, C. (2007) Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem. 105, 940–949.
- 4.
Cervantes-Paz, B., Yahia, E. M., Ornelas-Paz, J. J., Victoria-Campos, C. I., Ibarra-Junquera, V, Perez-Martinez, J. D., Escalante-Minakata, P. (2014) Antioxidant activity and content of chlorophylls and carotenoids in raw and heat-processed Jalapeño peppers at intermediate stages of ripening. Food Chem. 146, 188–196.
- 5.
Chudapongse, N., Kamkhunthod, M., Poompachee, K. (2010) Effects o. Phyllanthus urinaria extract on HepG2 cell viability and oxidative phosphorylation by isolated rat liver mitochondria. J. Ethnopharmacol. 130, 315–319.
- 6.
Egner, P. A., Munoz, A., Kensler, T. W. (2003) Chemoprevention with chlorophyll in individuals exposed to dietary aflatoxin. Mut. Res. 524, 209–216.
- 7.
Elliott, R. (2005) Mechanisms of genomic and non-genomic actions of carotenoids. Biochim. Biophys. Acta 1740, 147–154.
- 8.
Ferruzzi, M. G., Blakeslee, J. (2007) Digestion, absorption, and cancer preventative activity of dietary chlorophyll derivatives. Nutri. Res. 27, 1–12.
- 9.
Jayaraman, J. (2011) Estimation of Chlorophyll and Carotenoids. In Laboratory Manual in Biochemistry. New Age. Int. 1, 141–142.
- 10.
Lee, R. H., Cho, J. H., Jeon, Y. J., Bang, W., Cho, J. J., Choi, N. J., Seo, K. S., Shim, J. H., Chae, J. I. (2015) Quercetin induces antiproliferative activity against human hepatocellular carcinoma (HepG2) cells by suppressing specificity protein 1 (Sp1). Drug Dev. Res. doi:10.1002/ddr.21235.
- 11.
Maekawa, L., Lamping, R., Marcacci, M., Maekawa, M., Nassri, M., Koga, I. C. (2007) Antimicrobial activity of chlorophyll based solutions o. Candida albicans an. E. faecalis. RSBO 4, 36–40.
- 12.
Malick, C. P., Singh, M. B. (1980) Plant Enzymology and Histoenzymology. Kalyani Publishers, New Delhi.
- 13.
Rana, S. V., Pal, R., Vaiphei, K., Ola, R. P., Singh, K. (2010) Hepatoprotection by carotenoids in isoniazid-rifampicin induced hepatic injury in rats. Biochem. Cell Biol. 88, 819–834.
- 14.
Reddy, V. R., Bikshapathi, G., Reddy, K. M. (2014) Hepatoprotective activity of extract o. Erythroxylum monogynum in albino rats. Int. J. Pure Appl. Biosci. 2, 58–62.
- 15.
Sarkar, D., Sharma, A., Talukder, G. (1994) Chlorophyll and chlorophyllin as modifiers of genotoxic effects. Mut. Res. 318, 239–247.
- 16.
Shenoy, K. A., Soumyaji, S. N., Bairy, K. L. (2001) Hepatoprotective effect o. Ginkgo Biloba in carbon tetrachloride induced hepatic injury in rats. Indian J. Pharmacol. 33, 260–264.
- 17.
Singh, S., Mehta, A., Mehta, P. (2011) Hepatoprotective activity o. Cajanus cajan against carbon tetrachloride induced liver damage. Int. J. Pharm. Sci. 3, 146–147.
- 18.
Singh, N., Verma, P., Pandey, B. R. (2012) Therapeutic potential of organi. Triticum aestivum Linn. (wheat grass) in prevention and treatment of chronic diseases: An overview. Int. J. Pharm. Sci. Drug Res. 4, 10–14.
- 19.
Tang, Z. Y., Ye, S. L., Liu, Y. K., Qin, L. X., Sun, H. C., Ye, Q. H., Wang, L., Zhou, J., Qiu, S. J., Li, Y. (2004) A decade’s studies on metastasis of hepatocellular carcinoma. J. Cancer Res. Clin. Oncol. 130, 187–196.
- 20.
Yin, Q. H., Yan, F. X., Zu, X. Y., Wu, Y. H., Wu, X. P., Liao, M. C., Deng, S. W., Yin, L. I., Zhuang, Y. Z. (2012) Anti-proliferative and pro-apoptotic effect of carvacrol on human hepatocellular carcinoma cell line HepG-2. Cytotechnology 64, 43–51.
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Rajoria, A., Mehta, A., Mehta, P. et al. Evaluation of Antiproliferative and Hepatoprotective Effects of Wheat Grass (Triticum Aestivum). BIOLOGIA FUTURA 68, 150–161 (2017). https://doi.org/10.1556/018.68.2017.2.3
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Keywords
- T. aestivum (wheat grass)
- antiproliferative
- hepatoprotective
- apoptosis mechanism
- pharmacological significance