Acta Biologica Hungarica

, Volume 67, Issue 4, pp 412–423 | Cite as

Essential Oil Composition of Sweet Basil (Ocimum Basilicum L.) in Symbiotic Relationship with Piriformospora Indica and Paclobutrazol Application Under Salt Stress

  • Sara KeramatiEmail author
  • Hemmatollah Pirdashti
  • Valliollah Babaeizad
  • Ali Dehestani


Essential oil content and oil composition of paclobutrazol treated sweet basil (Ocimum basilicum L.) plant inoculated with Piriformospora indica under salt stress were investigated by GC-MS. The results show a slight increase in essential oil content when basil plants subjected to moderate salinity stress (3 dS m–1 of NaCl). It decreased significantly with increasing salinity level to 9 dS m–1. The findings revealed that leaf area, above ground and leaf dry weights, essential oil content and yield were significantly affected by P. indica inoculation, however paclobutrazol application significantly influenced essential oil yield but not content. Fungal symbiosis as well as paclobutrazol application ameliorated the negative effects of salinity on dry matter and essential oil yield. The main constituents found in the volatile oil of O. basilicum in control treatment were Geranial (26.03%), Neral (24.88%) and Estragole (24.78%). The compounds concentrations showed some differences in P. indica and paclobutrazol treatments. The results demonstrate that micorrhiza-like fungi concomitantly increase essential oil production and biomass in sweet basil, a medicinal herb rich in commercially valuable essential oils.


Essential oil GC-MS Ocimum basilicum paclobutrazol Piriformospora indica salinity 


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© Akadémiai Kiadó, Budapest 2016

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Authors and Affiliations

  • Sara Keramati
    • 1
    Email author
  • Hemmatollah Pirdashti
    • 1
  • Valliollah Babaeizad
    • 2
  • Ali Dehestani
    • 1
  1. 1.Department of Agronomy, Genetic and Agricultural Biotechnology Institute of TabarestanSari Agricultural Sciences and Natural Resources UniversitySariIran
  2. 2.Department of PhytopathologySari Agricultural Sciences and Natural Resources UniversitySariIran

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