Advertisement

Acta Biologica Hungarica

, Volume 67, Issue 4, pp 412–423 | Cite as

Essential Oil Composition of Sweet Basil (Ocimum Basilicum L.) in Symbiotic Relationship with Piriformospora Indica and Paclobutrazol Application Under Salt Stress

  • Sara KeramatiEmail author
  • Hemmatollah Pirdashti
  • Valliollah Babaeizad
  • Ali Dehestani
Article
  • 1 Downloads

Abstract

Essential oil content and oil composition of paclobutrazol treated sweet basil (Ocimum basilicum L.) plant inoculated with Piriformospora indica under salt stress were investigated by GC-MS. The results show a slight increase in essential oil content when basil plants subjected to moderate salinity stress (3 dS m–1 of NaCl). It decreased significantly with increasing salinity level to 9 dS m–1. The findings revealed that leaf area, above ground and leaf dry weights, essential oil content and yield were significantly affected by P. indica inoculation, however paclobutrazol application significantly influenced essential oil yield but not content. Fungal symbiosis as well as paclobutrazol application ameliorated the negative effects of salinity on dry matter and essential oil yield. The main constituents found in the volatile oil of O. basilicum in control treatment were Geranial (26.03%), Neral (24.88%) and Estragole (24.78%). The compounds concentrations showed some differences in P. indica and paclobutrazol treatments. The results demonstrate that micorrhiza-like fungi concomitantly increase essential oil production and biomass in sweet basil, a medicinal herb rich in commercially valuable essential oils.

Keywords

Essential oil GC-MS Ocimum basilicum paclobutrazol Piriformospora indica salinity 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Adams, R. P. (2007) Identification of essential oil components by gas chromatography/mass spectrometry. Allured Publ. Corp., San Diego.Google Scholar
  2. 2.
    Allen, M. F., Moore, Jr. T. S. Christensen, M. (1980) Phytohormone changes in Bouteloua gracilis infected by vesicular-arbuscular mycorrhizae: I. Cytokinin increases in the host plant. Can. J. Bot. 58, 371–374.CrossRefGoogle Scholar
  3. 3.
    Ansari, M. W., Trivedi, D. K., Sahoo, R. K., Gill, S. S., Tuteja, N. (2013) A critical review on fungi mediated plant responses with special emphasis to Piriformospora indica on improved production and protection of crops. Plant Physiol. Biochem. 70, 403–410.CrossRefGoogle Scholar
  4. 4.
    Ashoori, M., Ashraf, S., Alipour, Z. T. (2015) Investigating the effect of two species of mycorrhiza fungi and salinity on growth, function and chlorophyll content on Ocimum basilicum. Intl. J. Agri. Crop Sci. 8, 503–509.Google Scholar
  5. 5.
    Baltruschat, H., Fodor, J., Harrach, B. D., Niemczyk, E., Barna, B., Gullner, G., Janeczko, A., Kogel, K. H., Schäfer, P., Schwarczinger, I., Zuccaro, A. (2008) Salt tolerance of barley induced by the root endophyte Piriformospora indica is associated with a strong increase in antioxidants. New Phytol. 180, 501–510.CrossRefGoogle Scholar
  6. 6.
    Bañón, S., González, A., Cano, E. A., Franco, J. A., Fernández, J. A. (2002) Growth, development and colour response of potted Dianthus caryophyllus cv. Mondriaan to paclobutrazol treatment. Sci. Hortic. 94, 371–377.CrossRefGoogle Scholar
  7. 7.
    Bauer, K., Garbe, D., Surburg, H. (2008) Common fragrance and flavor materials: preparation, properties and uses. Wiley-VCH Verlag, Weinheim, Germany.Google Scholar
  8. 8.
    Carvalho Filho, J. L., Blank, A. F., Alves, P. B., Ehlert, P. A., Melo, A. S., Cavalcanti, S. C., Arrigoni-Blank, M. D., Silva-Mann, R. (2006) Influence of the harvesting time, temperature and drying period on basil (Ocimum basilicum L.) essential oil. Rev. Bras. Farmacogn. 16, 24–30.CrossRefGoogle Scholar
  9. 9.
    Copetta, A., Lingua, G., Berta, G. (2006) Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil production in Ocimum basilicum L. var. Genovese. Mycorrhiza 16, 485–494.CrossRefGoogle Scholar
  10. 10.
    Dolatabadi, H. K., Goltapeh, E. M., Moieni, A., Jaimand, K., Sardrood, B. P., Varma, A. (2011) Effect of Piriformospora indica and Sebacina vermifera on plant growth and essential oil yield in Thymus vulgaris in vitro and in vivo experiments. Symbiosis 53, 29–35.CrossRefGoogle Scholar
  11. 11.
    Ghahfarokhi, R. M., Goltapeh, M. E. (2010) Potential of the root endophytic fungus Piriformospora indica; Sebacina vermifera and Trichoderma species in biocontrol of take-all disease of wheat Gaeumannomyces graminis var. tritici in vitro. J. Agric. Technol. 6, 11–18.Google Scholar
  12. 12.
    Hajihashemi, S., Kiarostami, K., Enteshari, S., Saboora, A. (2006) The effects of salt stress and paclobutrazol on some physiological parameters of two salt tolerant and salt sensitive cultivars of wheat. Pak. J. Biol. Sci. 9, 1370–1374.CrossRefGoogle Scholar
  13. 13.
    Haque, M. A., Hossain, M. S., Rahman, M. Z., Rahman, M. R., Hossain, M. S., Mosihuzzaman, M., Nahar, N., Khan, S. I. (2005) Isolation of bioactive secondary metabolites from the endophytic fungus of Ocimum basilicum. J. Pharm. Sci. 4, 127–130.Google Scholar
  14. 14.
    Hussain, A. I., Anwar, F., Sherazi, S. T., Przybylski, R. (2008) Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food. Chem. 108, 986–995.CrossRefGoogle Scholar
  15. 15.
    Käfer, E. (1977) Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv. Genet. 19, 33–131.CrossRefGoogle Scholar
  16. 16.
    Manivannan, P., Jaleel, C. A., Kishorekumar, A., Sankar, B., Somasundaram, R., Panneerselvam, R. (2008) Protection of Vigna unguiculata (L.) Walp. plants from salt stress by paclobutrazol. Colloids Surf. B Biointerfaces, 61, 315–318.CrossRefGoogle Scholar
  17. 17.
    Morales, C., Cusido, R. M., Palazon, J., Bonfill, M. (1993) Tolerance of mint plants to soil salinity. J. Indian Soc. Soil Sci. 44, 184–186.Google Scholar
  18. 18.
    Oelmüller, R., Sherameti, I., Tripathi, S., Varma, A. (2009) Piriformospora indica, a cultivable root endophyte with multiple biotechnological applications. Symbiosis 49, 1–7.CrossRefGoogle Scholar
  19. 19.
    Sepahri, M., Saleh Rastin, N., Hossieni Salkedeh, G., Khayam Nekouie, M. (2009) Effect of endophytic fungus, Piriformospora indica, on growth and resistance of Hordeum vulgare L. to salinity stress. Rangeland 3, 508–518 (In Persian).Google Scholar
  20. 20.
    Sharafzadeh, S., Zare, M. (2011) Influence of growth regulators on growth and secondary metabolites of some medicinal plants from Lamiaceae family. Adv. Environ. Biol. 1, 2296–2303.Google Scholar
  21. 21.
    Sun, C., Johnson, J. M., Cai, D., Sherameti, I., Oelmüller, R., Lou, B. (2010) Piriformospora indica confers drought tolerance in Chinese cabbage leaves by stimulating antioxidant enzymes, the expression of drought-related genes and the plastid-localized CAS protein. J. Plant. Physiol. 167, 1009–1017.CrossRefGoogle Scholar
  22. 22.
    Vadassery, J., Ritter, C., Venus, Y., Camehl, I., Varma, A., Shahollari, B., Novák, O., Strnad, M., Ludwig-Müller, J., Oelmüller, R. (2008) The role of auxins and cytokinins in the mutualistic interaction between Arabidopsis and Piriformospora indica. Mol. Plant-Microbe Interact. 21, 1371–1383.CrossRefGoogle Scholar
  23. 23.
    Varma, A., Verma, S., Sahay, N., Bütehorn, B., Franken, P. (1999) Piriformospora indica, a cultivable plant-growth-promoting root endophyte. Appl. Environ. Microbiol. 65, 2741–2744.PubMedPubMedCentralGoogle Scholar
  24. 24.
    Vierheilig, H., Coughlan, A. P., Wyss, U., Piché, Y. (1998) Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi. Appl. Environ. Microbiol. 64, 5004–5007.PubMedPubMedCentralGoogle Scholar
  25. 25.
    Vos, C. M., Yang, Y., De Coninck, B., Cammue, B. P. (2014) Fungal (-like) biocontrol organisms in tomato disease control. Biol. Control, 74, 65–81.CrossRefGoogle Scholar
  26. 26.
    Wogiatzi, E., Papachatzis, A., Kalorizou, H., Chouliara, A., Chouliaras, N. (2011) Evaluation of essential oil yield and chemical components of selected basil cultivars. Biotechnol. Equip. 25, 2525–2527.CrossRefGoogle Scholar
  27. 27.
    World Health Organization. (2011) Traditional medicine fact sheet No 134. December, 2008.Google Scholar
  28. 28.
    Yeshitela, T., Robbertse, P. J., Stassen, P. J. (2004) Paclobutrazol suppressed vegetative growth and improved yield as well as fruit quality of ‘Tommy Atkins’ mango (Mangifera indica) in Ethiopia. N. Z. J. Crop Hortic. Sci. 32, 281–293.CrossRefGoogle Scholar
  29. 29.
    Zhang, H. W., Song, Y. C., Tan, R. X. (2006) Biology and chemistry of endophytes. Nat. Prod. Rep. 23, 753–771.CrossRefGoogle Scholar
  30. 30.
    Zuccarini, P., Okurowska, P. (2008) Effects of mycorrhizal colonization and fertilization on growth and photosynthesis of sweet basil under salt stress. J. Plant Nutr. 31, 497–513.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2016

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Sara Keramati
    • 1
    Email author
  • Hemmatollah Pirdashti
    • 1
  • Valliollah Babaeizad
    • 2
  • Ali Dehestani
    • 1
  1. 1.Department of Agronomy, Genetic and Agricultural Biotechnology Institute of TabarestanSari Agricultural Sciences and Natural Resources UniversitySariIran
  2. 2.Department of PhytopathologySari Agricultural Sciences and Natural Resources UniversitySariIran

Personalised recommendations