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Acta Biologica Hungarica

, Volume 67, Issue 3, pp 297–304 | Cite as

Preculturing Effect of Thidiazuron on In Vitro Shoot Multiplication and Micropropagation Round in Capparis Decidua (FORSK.) an Important Multipurpose Plant

  • Najat A. W. Bukhari
  • Iram SiddiqueEmail author
  • Kahkashan Perveen
Article

Abstract

An effcient protocol was developed for clonal multiplication of an important shrub: Capparis decidua (Forsk.) Edgew, through in vitro shoot induction and multiplication from nodal explants. Pretreatment of nodal explants in a liquid Murashige and Skoog (MS) medium augmented with various thidiazuron (TDZ) concentrations at relatively high levels (5–100 µM) for different time duration (4, 8, 12 and 16 d), proved a signifcant approach for in vitro shoot production. After an initial exposure time to TDZ, nodal explants were inoculated onto a MS basal medium devoid of TDZ for further induction and proliferation. The highest regeneration rate (85%), average number of shoots/explant (8.7 ± 0.22) and maximum shoot length (3.9 ± 0.33 cm) were obtained from the nodal explants exposed to 50 µM TDZ for 8 d. The nodal explants excised from the proliferated cultures of TDZ (50 µM) for 8 d were used as explants and showed an enhancement rate after next three round of in vitro propagation. Best results for rooting was obtained by ex vitro treatment of shoots with 200 µM indole-3-butyric acid (IBA) for 20 min. as it produced an average of 5.7 ± 0.41 roots per microshoot with 4.4 ± 0.39 cm root length in 84% shoots. Different planting substrates was tested for maximum survival of hardening off micropropagated plantlets and soilrite proved most effective than others as 97.1 ± 7.21 plantlets survived. All micropropagated plants grew well in natural conditions and showed similar morphology to the mother plant.

Keywords

Acclimatization Clonal multiplication Ex vitro Murashige and Skoog medium Planting substrate 

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References

  1. 1.
    Ahmad, N., Anis, M. (2007) Rapid plant regeneration protocol for cluster bean (Cyamopsis tetragonoloba L. Taub.). J. Hort. Sci. Biotechnol. 82, 585–589.CrossRefGoogle Scholar
  2. 2.
    Ahmad, N., Anis, M. (2011) An effcient in vitro process for recurrent production of cloned plants of Vitex negundo L. Eur. J. For. Res. 130, 135–144.CrossRefGoogle Scholar
  3. 3.
    Ahmed, V. U., Arif, S., Amber, A. R., Usmanghani, K., Mianna, G. A. (1989) A new spermidine alkaloid from C. decidua. Heterocycles 23, 3015–3020.CrossRefGoogle Scholar
  4. 4.
    Alatar, A. A. (2015) Thidiazuron induced effcient in vitro multiplication and ex vitro conservation of Rauvolfa serpentina– a potent antihypertensive drug producing plant. Biotech. Biotech. Equip. 29, 489–497.CrossRefGoogle Scholar
  5. 5.
    Anonymous (2001) Ker: A Genetic wealth of Haryana. CCS HAU. Research Courier 6, 4.Google Scholar
  6. 6.
    Bajaj, Y. P. S., Furmanova, M., Loszowska, O. (1988) Biotechnology of the micropropagation of medicinal and aromatic plants. In: Bajaj, Y. P. S. (ed.) Biotechnology in Agriculture and Forestry. Vol. I V. Medicinal and Aromatic Plants I, Springer-Verlag, Berlin, Heidelberg, New York, pp. 60–103.Google Scholar
  7. 7.
    Chahar, O. P., Kharb, P., Ali, S. F., Batra, P., Chowdhury, V. K. (2010) Development of protocol on micropropagation in Ker (Capparis decidua (Forsk) Edgew). World Appl. Sci. J. 10, 695–698.Google Scholar
  8. 8.
    Deora, N. S., Shekhawat, N. S. (1995) Micropropagation of Capparis decidua (Forsk) Edgew; a tree of arid horticulture. Plant Cell Rep. 15, 278–281.CrossRefGoogle Scholar
  9. 9.
    Faisal, M., Singh, S., Anis, M. (2005) In vitro regeneration and plant establishment of Tylophora indica (Burm. f.) Merrill: petiole callus culture. In Vitro Cell. Dev. Biol. Plant 41, 511–515.CrossRefGoogle Scholar
  10. 10.
    Gupta, I. C., Harsh, L. N., Sharikarnarayan, K. A., Sharma, B. D. (1989) Wealth from wastelands. Ind. Farm. 38, 18–19.Google Scholar
  11. 11.
    Huetteman, C. A., Preece, J. E. (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. Cult. 33, 105–119.CrossRefGoogle Scholar
  12. 12.
    Khan, T. I., Dular, A. K., Soloman, D. M. (2003) Biodiversity in Thar desert with emphasis on endemic and medicinal plants. Environ. 23, 137–144.Google Scholar
  13. 13.
    Mithila, J., Murch, S. J., Krishnaraj, S., Saxena, P. K. (2001) Recent advances in Pelargonium in vitro regeneration systems. Plant Cell Tiss. Org. Cult. 67, 1–9.CrossRefGoogle Scholar
  14. 14.
    Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 15, 473–497.CrossRefGoogle Scholar
  15. 15.
    Murch, S. J., Choffe, K. L., Victor, J. M. R., Slimmon, T. Y., KrishnaRaj, S., Saxena, P. K. (2000) Thidiazuron induced plant regeneration from hypocotyl cultures of St. Johns wort (Hypericum perfo-ratum L. cv. Anthos). Plant Cell Rep. 19, 576–581.CrossRefGoogle Scholar
  16. 16.
    Olmez, Z., Yahyao, Z., Ucler, O. (2004) Effect of H2SO4, KNO3 and GA3 treatments on germination of Caper (Capparis ovata Desf.) seeds. Pak. J Biol. Sci. 7, 872–882.Google Scholar
  17. 17.
    Onofrio, C. D., Morini, S. (2005) Development of adventitious shoots from in vitro grown Cydonia oblonga leaves as infuenced by different cytokinins and treatment duration. Biol. Plant. 49, 17–21.CrossRefGoogle Scholar
  18. 18.
    Purohit, S. D., Dave, A., Kukda, G. (1994) Micropropagation of safed musli (Chlorophytum borivilianum)–a rare Indian medicinal plant. Plant Cell Tiss. Org. Cult. 39, 93–96.CrossRefGoogle Scholar
  19. 19.
    Rivera, D., Inocencio, C., Obon, C., Alcaraz, F. (2003) Review of food and medicinal uses of Capparis L. subgenus Capparis (Capparidaceae). Eco. Bot. 57, 515–534.CrossRefGoogle Scholar
  20. 20.
    Rout, G. R. (2002) Direct plant regeneration from leaf explants of Plumbago species and its genetic fdelity through RAPD markers. Annals Appl. Biol. 140, 305–313.CrossRefGoogle Scholar
  21. 21.
    Rout, G. R., Reddy, G. M., Das, P. (2001) Studies on in vitro clonal propagation of Paulownia tomentosa Steud, and evaluation of genetic fdelity through RAPD marker. Silvae Genet. 50, 208–212.Google Scholar
  22. 22.
    Sharma, B., Kumar, P. (2009) In vitro antifungal potency of some plant extracts against Fusarium oxysporum. Int. J. Green Pharm. 3, 63–65.CrossRefGoogle Scholar
  23. 23.
    Shekhawat, M. S., Manokari, M., Ravindran, C. P. (2015) An improved micropropagation protocol by ex vitro rooting of Passifora edulis Sims. f. favicarpa Deg. through nodal segment culture. Scientifca article id 578676, p. 1–8.Google Scholar
  24. 24.
    Siddique, I., Bukhari, N. A., Perveen, K., Siddiqui, I., Anis, M. (2013) Preculturing of nodal explants in thidiazuron supplemented liquid medium improves in vitro shoot multiplication of Cassia angustifolia. Acta Biol. Hung. 64, 377–384.CrossRefGoogle Scholar
  25. 25.
    Singh, D., Singh, R. K. (2011) Kair (Capparis decidua): A potential ethnobotanical weather predictor and livelihood security shrub of the arid zone of Rajasthan and Gujrat. Indian J. Tradit. Knowl. 10, 146–155.Google Scholar
  26. 26.
    Tyagi, P., Kothari, S. L. (1997) Micropropagation of Capparis decidua through in vitro shoot proliferation on nodal explants of mature tree and seedlings explants. J. Plant Biochem. Biotechnol. 6, 19–23.CrossRefGoogle Scholar
  27. 27.
    Vijay, N., Arya, S., Arya, I. D. (2014) Rapid and mass propagation of the economically important desert plant Capparis decidua for its afforestation program. J. Arid Land Studies 24, 33–36.Google Scholar
  28. 28.
    Yadav, K., Singh, N. (2011) Effect of seed harvesting season and sterilization treatments on germination and in vitro propagation of Albizia lebbeck (L.) Benth. Analele Universitatii din Oradea–Fascicula Biologie 18, 151–156.Google Scholar
  29. 29.
    Yadav, K., Singh, N., Verma, S. (2012) Plant tissue culture: a biotechnological tool for solving the problem of propagation of multipurpose endangered medicinal plants in India. J. Agricul. Technol, 8, 305–318.Google Scholar

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© Akadémiai Kiadó, Budapest 2016

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Najat A. W. Bukhari
    • 1
  • Iram Siddique
    • 1
    Email author
  • Kahkashan Perveen
    • 1
  1. 1.Department of Botany and Microbiology, College of Science, Female Centre for Scientifc and Medical CollegesKing Saud UniversityRiyadhKingdom of Saudi Arabia

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