Acta Biologica Hungarica

, Volume 67, Issue 3, pp 297–304 | Cite as

Preculturing Effect of Thidiazuron on In Vitro Shoot Multiplication and Micropropagation Round in Capparis Decidua (FORSK.) an Important Multipurpose Plant

  • Najat A. W. Bukhari
  • Iram SiddiqueEmail author
  • Kahkashan Perveen


An effcient protocol was developed for clonal multiplication of an important shrub: Capparis decidua (Forsk.) Edgew, through in vitro shoot induction and multiplication from nodal explants. Pretreatment of nodal explants in a liquid Murashige and Skoog (MS) medium augmented with various thidiazuron (TDZ) concentrations at relatively high levels (5–100 µM) for different time duration (4, 8, 12 and 16 d), proved a signifcant approach for in vitro shoot production. After an initial exposure time to TDZ, nodal explants were inoculated onto a MS basal medium devoid of TDZ for further induction and proliferation. The highest regeneration rate (85%), average number of shoots/explant (8.7 ± 0.22) and maximum shoot length (3.9 ± 0.33 cm) were obtained from the nodal explants exposed to 50 µM TDZ for 8 d. The nodal explants excised from the proliferated cultures of TDZ (50 µM) for 8 d were used as explants and showed an enhancement rate after next three round of in vitro propagation. Best results for rooting was obtained by ex vitro treatment of shoots with 200 µM indole-3-butyric acid (IBA) for 20 min. as it produced an average of 5.7 ± 0.41 roots per microshoot with 4.4 ± 0.39 cm root length in 84% shoots. Different planting substrates was tested for maximum survival of hardening off micropropagated plantlets and soilrite proved most effective than others as 97.1 ± 7.21 plantlets survived. All micropropagated plants grew well in natural conditions and showed similar morphology to the mother plant.


Acclimatization Clonal multiplication Ex vitro Murashige and Skoog medium Planting substrate 


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© Akadémiai Kiadó, Budapest 2016

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Najat A. W. Bukhari
    • 1
  • Iram Siddique
    • 1
    Email author
  • Kahkashan Perveen
    • 1
  1. 1.Department of Botany and Microbiology, College of Science, Female Centre for Scientifc and Medical CollegesKing Saud UniversityRiyadhKingdom of Saudi Arabia

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