Anti-Listerial Effect of Selected Essential Oils and Thymol

Abstract

The anti-listerial effect of marjoram, thyme essential oils (EOs) and thymol on Listeria monocytogenes inoculated chicken breast fllets was investigated. Before inoculation the fllets were pretreated by washing or not under running tap water. Inoculated samples were kept at 6 °C for 24 h to allow the growth of L. monocytogenes. After this, the fllets were put in marinating solutions containing salt (5%) and EOs or thymol in MIC/2 concentration established in vitro. Total germ count (TGC) and L. monocytogenes count was monitored on the meat surface and in the marinating solutions following 24 and 48 h storage at 6 °C. Thyme and thymol reduced signifcantly Listeria cell count (1–3 log CFU) in both samples. They also gave good favour to the fried meat. The doses of EOs used were optimal for antimicrobial effciency and had a pleasant favour effect. Washing was not effcient in reducing total germ count.

References

  1. 1.

    Bakkali, F., Averbeck, S., Averbeck, D., Idaomar, M. (2008) Biological effects of essential oils–a review. Food Chem. Toxicol. 46, 446–475.

    CAS  Article  Google Scholar 

  2. 2.

    Beresford, M. R., Andrew, P. W., Shama, G. (2001) Listeria monocytogenes adheres to many materials found in food-processing environments. J Appl. Microbiol. 90, 1000–1005.

    CAS  Article  Google Scholar 

  3. 3.

    Burt, S. (2004) Essential oils: their antibacterial properties and potential applications in foods–a review. Int. J. Food Microbiol. 94, 223–253.

    CAS  Article  Google Scholar 

  4. 4.

    De Oliveira, M. M. M., Florisvaldo Brugnera, D., Das Graças Cardoso, M., Alves, E., Hilsdorf Piccoli, R. (2010) Bioflm formation by Listeria monocytogenes on stainless steel surface and biotransfer potential. Braz. J. Microbiol. 41, 97–106.

    Article  Google Scholar 

  5. 5.

    Farber, J. M., Peterkin, P. (1991) Listeria monocytogenes, a food-borne pathogen. Microbiol. Rev. 55, 476–511.

    CAS  PubMed  PubMed Central  Google Scholar 

  6. 6.

    Food Standard Agency (2014) Don’t wash raw chicken. Retrieved from https://doi.org/www.food.gov.uk/news-updates/campaigns/campylobacter/fsw-2014news-updates/campaigns/campylobacter/fsw-2014

    Google Scholar 

  7. 7.

    Gandhi, M., Chikindas, M. L. (2007) Listeria: A foodborne pathogen that knows how to survive. Int. J. Food Microbiol. 113, 1–15.

    Article  Google Scholar 

  8. 8.

    Høiby, N., Bjarnsholt, T., Givskov, M., Molin, S., Ciofu, O. (2010). Antibiotic resistance of bacterial bioflms. Int. J. Antimicrob. Agents 35, 322–332.

    Article  Google Scholar 

  9. 9.

    Hyldgaard, M., Mygind, T., Meyer, R. L. (2012) Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Front. Microbiol. 3, 12.

    Article  Google Scholar 

  10. 10.

    Johnson, L. R. (2007) Microcolony and bioflm formation as a survival strategy for bacteria. J. Theor. Biol. 251, 24–34.

    Article  Google Scholar 

  11. 11.

    Kerekes, E.-B., Deák, É., Takó, M., Tserennadmid, R., Petkovits, T., Vágvölgyi, C., Krisch, J. (2013) Anti-bioflm forming and anti-quorum sensing activity of selected essential oils and their main components on food-related micro-organisms. J. Appl. Microbiol. 115, 933–942.

    CAS  PubMed  Google Scholar 

  12. 12.

    Khanjari, A., Karabagias, I. K., Kontominas, M. G. (2013) Combined effect of N,O-carboxymethyl chitosan and oregano essential oil to extend shelf life and control Listeria monocytogenes in raw chicken meat fllets. LWT–Food Sci. Technol. 53, 94–99.

    CAS  Article  Google Scholar 

  13. 13.

    Noriega, E., Shama, G., Laca, A., Díaz, M., Kong, M. G. (2011) Cold atmospheric gas plasma disinfection of chicken meat and chicken skin contaminated with Listeria innocua. Food Microbiol. 28, 1293–300.

    CAS  Article  Google Scholar 

  14. 14.

    Mead, P. S., Dunne, E. F., Graves, L., Wiedmann, M., Patrick, M., Hunter, S., Salehi, E., Mostashari, F., Craig, A., Mshar, P., Bannerman, T., Sauders, B. D., Hayes, P., Dewitt, W., Sparling, P., Griffn, P., Morse, D., Slutsker, L., Swaminathan, B. (2006) Nationwide outbreak of listeriosis due to contaminated meat. Epidemiol. Infect. 134, 744–751.

    CAS  Article  Google Scholar 

  15. 15.

    Rasooli, I., Rezaei, M. B., Allameh, A. (2006) Ultrastructural studies on antimicrobial effcacy of thyme essential oils on Listeria monocytogenes. Int. J. Infect. Dis. 10, 236–241.

    Article  Google Scholar 

  16. 16.

    Rendueles, O., Ghigo, J.-M. (2012) Multi-species bioflms: how to avoid unfriendly neighbors. FEMS Microbiol. Rev. 36, 972–989.

    CAS  Article  Google Scholar 

  17. 17.

    Sakaridis, I., Soultos, N., Dovas, C. I., Papavergou, E., Ambrosiadis, I., Koidis, P. (2012) Lactic acid bacteria from chicken carcasses with inhibitory activity against Salmonella spp. and Listeria monocy-togenes. Anaerobe 18, 62–66.

    CAS  Article  Google Scholar 

  18. 18.

    Tompkin, R. B. (2002). Control of Listeria monocytogenes in the food-processing environment. J. Food Prot. 65, 709–725.

    CAS  Article  Google Scholar 

  19. 19.

    Tserennadmid, R., Takó, M., Galgóczy, L., Papp, T., Pesti, M., Vágvölgyi, C., Almássy, K., Krisch, J. (2011) Anti yeast activities of some essential oils in growth medium, fruit juices and milk. Int. J. Food Microbiol. 144, 480–486.

    CAS  Article  Google Scholar 

  20. 20.

    United States Department Of Agriculture (2013) Washing food: does it promote food safety? Retrieved from https://doi.org/www.fsis.usda.gov/wps/portal/fsis/topics/food-safety-education/get-answers/food-safety-fact-sheets/safe-food-handling/washing-food-does-it-promote-food-safety/washing-foodwps/portal/fsis/topics/food-safety-education/get-answers/food-safety-fact-sheets/safe-food-handling/washing-food-does-it-promote-food-safety/washing-food

    Google Scholar 

  21. 21.

    Verma, R. S., Padalia, R. C., Chauhan, A. (2013) Introduction of Cymbopogon distans (Nees ex Steud.) Wats to the sub-tropical India: Evaluation of essential-oil yield and chemical composition during annual growth. Ind.Crop.Prod. 49, 858–863.

    CAS  Article  Google Scholar 

  22. 22.

    Verma, R. S., Padalia, R. C., Chauhan, A. (2014) Chemical composition variability of essential oil during ontogenesis of Daucus carota L. subsp. sativus (Hoffm.) Arcang. Ind.Crop.Prod. 52, 809–814.

    CAS  Article  Google Scholar 

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Correspondence to Erika-Beáta Kerekes.

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Kerekes, EB., Vidács, A., Török, J.J. et al. Anti-Listerial Effect of Selected Essential Oils and Thymol. BIOLOGIA FUTURA 67, 333–343 (2016). https://doi.org/10.1556/018.67.2016.3.10

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Keywords

  • Listeria
  • essential oils
  • chicken meat
  • sensory evaluation