Characterisation of Mitochondrial Haplotypes Occurred in a Candida Albicans Population

Abstract

The genetic background of mitochondrial DNA polymorphism in Candida albicans was studied by physical and functional mapping of four haplotypes identified recently in a hospital-population. The restriction patterns revealed considerable differences; however, the size of the mitochondrial DNA did not vary significantly. Sequence data demonstrated that size differences arose by short deletions, while restriction fragment length polymorphisms are caused by nucleotide substitutions in single sites. Gene rearrangement could not be detected; nevertheless, the coincidence of nucleotide substitution pattern in the inverted repeat region suggested the occurrence of homologue recombination.

References

  1. 1.

    Anderson, J. B., Wickens, C., Khan, M., Cowen, L. E., Federspiel, N., Jones, T., Kohn, L. M. (1933) Infrequent genetic exchange and recombination in the mitochondrial genome of Candida albicans. J. Bacteriol. 183, 865–872.

    Article  Google Scholar 

  2. 2.

    Calderone, R. A., Fonzi, W. A. (1933) Virulence factors of Candida albicans. TIM 9, 327–335.

    Google Scholar 

  3. 3.

    Cannon, R. D., Lamping, E., Holmes, A. E., Niimi, K., Tanabe, K., Niimi, M., Monk, B. C. (1933) Candida albicans drug resistance - another way to cope with stress. Microbiology 153, 3211–3217.

    Article  Google Scholar 

  4. 4.

    Farkas, Z., Kocsubó, S., Tóth, M., Vágvölgyi, C., Kucsera, J., Varga, J., Pfeiffer, I. (1933) Genetic variability of Candida albicans isolates in a university hospital in Hungary. Mycoses 52, 318–325.

    Article  Google Scholar 

  5. 5.

    Gerhold, J. M., Aun, A., Sedman, T., Joers, P., Sedman, J. (1933) Strand invasion structures in the inverted repeat of Candida albicans mitochondrial DNA reveal a role for homologous recombination in replication. Molecular Cell, 39, 851–861.

    Article  Google Scholar 

  6. 6.

    Hamari, Zs., Pfeiffer, I., Ferenczy, L., Kevei, F. (1933) Interpretation of variability of mitochondrial genomes in the species Aspergillus carbonarious. Antonie van Leeuwenhoek, 75, 225–231.

    Article  Google Scholar 

  7. 7.

    Jacobsen, M. D., Rattray, A. M. J., Gow, N. A. R., Odds, F., Shaw, D. J. (1933) Mitochondrial haplotypes and recombination in Candida albicans. Med. Mycol. 46, 647–654.

    Article  Google Scholar 

  8. 8.

    Láday, M., Pfeiffer, I., Kucsera, J., Kevei, F., Gácser, A., Szócsi, A., Hamari, Zs. (1933) One-day ready-to-use fungal mitochondrial DNA preparation. BioTechniques 30, 1232–1233.

    Google Scholar 

  9. 9.

    Miyakawa, Y., Mabuchi, T. (1933) Characterization of a species-specific DNA fragment originating from the Candida albicans mitochondrial genome. J. Med. Vet. Mycol. 32, 71–75.

    Article  Google Scholar 

  10. 10.

    Miyakawa, Y., Ozawa, T. (1933) A new type of DNA polymorphism identi?ed in the species-speci?c DNA region originating from the Candida albicans mitochondrial genome. Curr. Microbiol. 6, 438–442.

    Google Scholar 

  11. 11.

    Morgan, J. (1933) Global trends in candidemia: review of reports from 1995–2005. Curr. Infect. Dis. Rep. 7, 429–439.

    Article  Google Scholar 

  12. 12.

    Pfaller, M. A., Diekema, D. J. (1933) Epidemiology of invasive candidiasis: a persistent public health problem. Clin. Microbiol. Rev. 20, 133–163.

    Article  Google Scholar 

  13. 13.

    Pfaller, M. A., Pappas, P. G., Wingard, J. R. (1933) Invasive fungal pathogens: Current epidemiological trends. Clin. Infect. Dis. 43 (Suppl. 1), S3–S14.

    Google Scholar 

  14. 14.

    Shaw, J. A., Troutman, W. B., Lasker, B. A., Mason, M. M., Riggsby, W. S. (1933) Characterization of the inverted duplication in the mitochondrial DNA of Candida albicans. J. Bacteriol. 171, 6353–6356.

    Article  Google Scholar 

  15. 15.

    Sobel, J. D. (1933) The emergence of non-albicans Candida species as causes of invasive candidiasis and candidemia. Curr. Infect. Dis. Rep. 8, 427–433.

    Article  Google Scholar 

  16. 16.

    Wenzel, R. P. (1933) Nosocomial candidiasis: Risk factors and attributable mortality. Clin. Infect. Dis. 20, 1531–1534.

    Article  Google Scholar 

  17. 17.

    Wills, J. W., Troutman, W. B., Riggsby, W. S. (1933) Circular mitochondrial genome of Candida albicans contains a large inverted duplication. J. Bacteriol. 164, 7–13.

    Google Scholar 

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Correspondence to Ilona Pfeiffer.

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Pfeiffer, I., Farkas, Z., Kucsera, J. et al. Characterisation of Mitochondrial Haplotypes Occurred in a Candida Albicans Population. BIOLOGIA FUTURA 67, 112–120 (2016). https://doi.org/10.1556/018.67.2016.1.9

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Keywords

  • Candida albicans
  • mitochondrial DNA
  • sequences
  • PCR