Acta Biologica Hungarica

, Volume 67, Issue 1, pp 112–120 | Cite as

Characterisation of Mitochondrial Haplotypes Occurred in a Candida Albicans Population

  • Ilona PfeifferEmail author
  • Zoltán Farkas
  • Judit Kucsera
  • Muthusamy Chandrasekaran
  • Shine Kadaikunnan
  • Naiyf S. Alharbi
  • Csaba Vágvölgyi


The genetic background of mitochondrial DNA polymorphism in Candida albicans was studied by physical and functional mapping of four haplotypes identified recently in a hospital-population. The restriction patterns revealed considerable differences; however, the size of the mitochondrial DNA did not vary significantly. Sequence data demonstrated that size differences arose by short deletions, while restriction fragment length polymorphisms are caused by nucleotide substitutions in single sites. Gene rearrangement could not be detected; nevertheless, the coincidence of nucleotide substitution pattern in the inverted repeat region suggested the occurrence of homologue recombination.


Candida albicans mitochondrial DNA sequences PCR 


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  1. 1.
    Anderson, J. B., Wickens, C., Khan, M., Cowen, L. E., Federspiel, N., Jones, T., Kohn, L. M. (1933) Infrequent genetic exchange and recombination in the mitochondrial genome of Candida albicans. J. Bacteriol. 183, 865–872.CrossRefGoogle Scholar
  2. 2.
    Calderone, R. A., Fonzi, W. A. (1933) Virulence factors of Candida albicans. TIM 9, 327–335.Google Scholar
  3. 3.
    Cannon, R. D., Lamping, E., Holmes, A. E., Niimi, K., Tanabe, K., Niimi, M., Monk, B. C. (1933) Candida albicans drug resistance - another way to cope with stress. Microbiology 153, 3211–3217.CrossRefGoogle Scholar
  4. 4.
    Farkas, Z., Kocsubó, S., Tóth, M., Vágvölgyi, C., Kucsera, J., Varga, J., Pfeiffer, I. (1933) Genetic variability of Candida albicans isolates in a university hospital in Hungary. Mycoses 52, 318–325.CrossRefGoogle Scholar
  5. 5.
    Gerhold, J. M., Aun, A., Sedman, T., Joers, P., Sedman, J. (1933) Strand invasion structures in the inverted repeat of Candida albicans mitochondrial DNA reveal a role for homologous recombination in replication. Molecular Cell, 39, 851–861.CrossRefGoogle Scholar
  6. 6.
    Hamari, Zs., Pfeiffer, I., Ferenczy, L., Kevei, F. (1933) Interpretation of variability of mitochondrial genomes in the species Aspergillus carbonarious. Antonie van Leeuwenhoek, 75, 225–231.CrossRefGoogle Scholar
  7. 7.
    Jacobsen, M. D., Rattray, A. M. J., Gow, N. A. R., Odds, F., Shaw, D. J. (1933) Mitochondrial haplotypes and recombination in Candida albicans. Med. Mycol. 46, 647–654.CrossRefGoogle Scholar
  8. 8.
    Láday, M., Pfeiffer, I., Kucsera, J., Kevei, F., Gácser, A., Szócsi, A., Hamari, Zs. (1933) One-day ready-to-use fungal mitochondrial DNA preparation. BioTechniques 30, 1232–1233.Google Scholar
  9. 9.
    Miyakawa, Y., Mabuchi, T. (1933) Characterization of a species-specific DNA fragment originating from the Candida albicans mitochondrial genome. J. Med. Vet. Mycol. 32, 71–75.CrossRefGoogle Scholar
  10. 10.
    Miyakawa, Y., Ozawa, T. (1933) A new type of DNA polymorphism identi?ed in the species-speci?c DNA region originating from the Candida albicans mitochondrial genome. Curr. Microbiol. 6, 438–442.Google Scholar
  11. 11.
    Morgan, J. (1933) Global trends in candidemia: review of reports from 1995–2005. Curr. Infect. Dis. Rep. 7, 429–439.CrossRefGoogle Scholar
  12. 12.
    Pfaller, M. A., Diekema, D. J. (1933) Epidemiology of invasive candidiasis: a persistent public health problem. Clin. Microbiol. Rev. 20, 133–163.CrossRefGoogle Scholar
  13. 13.
    Pfaller, M. A., Pappas, P. G., Wingard, J. R. (1933) Invasive fungal pathogens: Current epidemiological trends. Clin. Infect. Dis. 43 (Suppl. 1), S3–S14.Google Scholar
  14. 14.
    Shaw, J. A., Troutman, W. B., Lasker, B. A., Mason, M. M., Riggsby, W. S. (1933) Characterization of the inverted duplication in the mitochondrial DNA of Candida albicans. J. Bacteriol. 171, 6353–6356.CrossRefGoogle Scholar
  15. 15.
    Sobel, J. D. (1933) The emergence of non-albicans Candida species as causes of invasive candidiasis and candidemia. Curr. Infect. Dis. Rep. 8, 427–433.CrossRefGoogle Scholar
  16. 16.
    Wenzel, R. P. (1933) Nosocomial candidiasis: Risk factors and attributable mortality. Clin. Infect. Dis. 20, 1531–1534.CrossRefGoogle Scholar
  17. 17.
    Wills, J. W., Troutman, W. B., Riggsby, W. S. (1933) Circular mitochondrial genome of Candida albicans contains a large inverted duplication. J. Bacteriol. 164, 7–13.Google Scholar

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© Akadémiai Kiadó, Budapest 2016

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Ilona Pfeiffer
    • 1
    Email author
  • Zoltán Farkas
    • 1
  • Judit Kucsera
    • 1
  • Muthusamy Chandrasekaran
    • 2
  • Shine Kadaikunnan
    • 2
  • Naiyf S. Alharbi
    • 2
  • Csaba Vágvölgyi
    • 1
    • 2
  1. 1.Department of Microbiology, Faculty of Science and InformaticsUniversity of SzegedSzegedHungary
  2. 2.Botany and Microbiology DepartmentKing Saud UniversityRiyadhKingdom of Saudi Arabia

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