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Acta Biologica Hungarica

, Volume 67, Issue 1, pp 53–63 | Cite as

Plant Growth Regulators Induced Urease Activity in Cucurbita Pepo L. Cotyledons

  • Hamed M. El Shora
  • Awatif S. Ali
Article

Abstract

This study is aimed to investigate the activity of urease (EC 3.5.1.5, urea amidohydrolase) that catalyzes the hydrolysis of urea in 5-day-old Cucurbita pepo cotyledons subjected to various concentrations of different growth regulators. The treatment of C. pepo cotyledons with different concentrations (100–600 µmol) of different auxins [indole-3-acetic acid (IAA), indole butyric acid (IBA), indole propionic acid (IPA) and naphthalene acetic acid (NAA)]; or with different concentrations (100–300 µmol) of different cytokinins [kinetin, zeatin and benzyladenine (6-BA)] resulted in a significant increase of urease activity, compared to control. The optimal effects were recorded for each of 500 µmol of IAA and 300 µmol of zeatin treatments. A gradual increase in urease activity was detected in cotyledons treated with various concentrations (0.2–1.0 mM) of 28-homobrassinolide (HBL), in relative to control. A substantial increase in urease activity was observed in cotyledons subjected to different concentrations of triazole (10–60 mg L–1), containing either triadimefon (TDM) or hexaconazole (HEX), compared to control. The combination of 300 µmol zeatin with any of protein inhibitors, namely 5-fluorouridine (FUrd), cordycepin and a-amanitin, resulted in the alleviation of their inhibitory effect on the urease activity.

Keywords

Auxins benzyladenine brassinosteroids cordycepin triazole urease activity zeatin 

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© Akadémiai Kiadó, Budapest 2016

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Hamed M. El Shora
    • 1
  • Awatif S. Ali
    • 2
  1. 1.Department of Botany, Faculty of ScienceMansoura UniversityMansouraEgypt
  2. 2.Department of Botany, Faculty of ScienceKafr El Sheikh UniversityKafr El SheikhEgypt

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