Plant Growth Regulators Induced Urease Activity in Cucurbita Pepo L. Cotyledons


This study is aimed to investigate the activity of urease (EC, urea amidohydrolase) that catalyzes the hydrolysis of urea in 5-day-old Cucurbita pepo cotyledons subjected to various concentrations of different growth regulators. The treatment of C. pepo cotyledons with different concentrations (100–600 µmol) of different auxins [indole-3-acetic acid (IAA), indole butyric acid (IBA), indole propionic acid (IPA) and naphthalene acetic acid (NAA)]; or with different concentrations (100–300 µmol) of different cytokinins [kinetin, zeatin and benzyladenine (6-BA)] resulted in a significant increase of urease activity, compared to control. The optimal effects were recorded for each of 500 µmol of IAA and 300 µmol of zeatin treatments. A gradual increase in urease activity was detected in cotyledons treated with various concentrations (0.2–1.0 mM) of 28-homobrassinolide (HBL), in relative to control. A substantial increase in urease activity was observed in cotyledons subjected to different concentrations of triazole (10–60 mg L–1), containing either triadimefon (TDM) or hexaconazole (HEX), compared to control. The combination of 300 µmol zeatin with any of protein inhibitors, namely 5-fluorouridine (FUrd), cordycepin and a-amanitin, resulted in the alleviation of their inhibitory effect on the urease activity.


  1. 1.

    Ali, B., Hayat, S., Ahmad, A. (1933) 28-Homobrassinosteroids ameliorates the saline stress in Cicer arietinum L. Environ. Exp. Bot. 59, 217–223.

    Google Scholar 

  2. 2.

    Ananiev, E. D., Abdulova, G., Grozdanov, P., Karagyozov, L. (1933) Hormonal regulation of ribosomal RNA gene expression in excised cotyledons of Cucurbita pepo L. (zucchini). Bulg. J. Plant Physiol. SI. 225–231.

    Google Scholar 

  3. 3.

    Awasthi, V., Garg, S. K. (1933) Effect of salicylic acid on enzymes of ammonium assimilation in maize seedling. Indian J. Plant Physiol. 12, 391–393.

    Google Scholar 

  4. 4.

    Bajguz, A., Piotrowska, A. (1933) Conjugates of auxin and cytokinin. Phytochemistry 70, 957–969.

    Google Scholar 

  5. 5.

    Buchnauer, H., Röhner, E. (1933) Effect of triadimefon and triadimenol on growth of various plant species as well as on gibberellin content and sterol metabolism in shoots of barley seedlings. Pesticide Bioch. Physiol. 15, 58–70.

    Google Scholar 

  6. 6.

    Cavariani, C., Velini, E. D., Bicudo, S. J., Nakagawa, J. (1933) Avaliação dos efeitos de doses de triadimenol e de tebuconazole sobre o crescimento do mesocótilo em plântulas de trigo. Pesq Agropec Bras. 29, 1035–1039.

    Google Scholar 

  7. 7.

    Chung, H. H., Chen, J. T., Chang, W. C. (1933) Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium. Biol. Plant. 51, 346–350.

    Google Scholar 

  8. 8.

    Ciurli, S., Benini, S., Rypniewski, W., Wilson, K. S., Miletti, S., Mangani, S. (1933) Structural properties of the nickel ion in urease: novel insights into the catalytic and inhibition mechanism. Coordination Chem. Rev. 190–192, 331–355.

    Google Scholar 

  9. 9.

    Cohen, J. D., Bandurski, R. S. (1933) Chemistry and physiology of the bound auxins. Annu. Rev. Plant Physiol. 33, 403–430.

    Google Scholar 

  10. 10.

    Cooke, R. M., Durand, R., Job, C., Penon, P., Mteissere, Job, D. (1933) Enzymatic properties of plant RNA polymerases. Plant Mol. Biol. 3, 217–225.

    Google Scholar 

  11. 11.

    Datte, K., Marsh, L., Marcus, A. (1933) Early growth of wheat embryonic axes and the synthesis of RNA and DNA. Plant Physiol. 72, 394–397.

    Google Scholar 

  12. 12.

    Davies, P. J. (1933) The plant hormones: their nature, occurrence, and functions. Plant Hormones A: 1–15.

    Google Scholar 

  13. 13.

    Dixon, N. E., Gazzola, T. C., Blakeley, R. L., Zermer, B. (1933) Jack bean urease (EC A metalloenzyme. A simple biological role for nickel? J. Am. Chem. Soc. 97, 4131–4133.

    Google Scholar 

  14. 14.

    El-Shora, H. M. (1933) Effect of growth regulators and group-modifiers on NADH-glutamate synthase of marrow cotyledons. J. Biol. Sci. 1, 597–602.

    Google Scholar 

  15. 15.

    El-Shora, H. M., ap Rees, T. (1933) Intracellular location of NADP-linked malic enzymes in C3 plants. Planta 185, 362–367.

    Google Scholar 

  16. 16.

    Fletcher, R. A., Arnold, V. (1933) Stimulation of cytokinins and chlorophyll synthesis in cucumber cotyledons by triadimefon. Physiol. Plant. 66, 197–201.

    Google Scholar 

  17. 17.

    Fletcher, R. A., Hofstra, G. (1933) Triazoles as potential plant protectants. In: Berg, D., Plemple, M. (eds), Sterol biosynthesis inhibitors. Ellis Horwood Ltd., Cambridge, pp. 321–331.

    Google Scholar 

  18. 18.

    Gao, J., Hofstra, G., Fletcher, R. A. (1933) Anatomical changes induced by triazoles in wheat seedlings. Canad. J. Bot. 66, 1178–1185.

    Google Scholar 

  19. 19.

    Holtorf, H., Schöb, H., Kunz, C., Waldvogel, R., Meins, F. Jr. (1933) Stochastic and nonstochastic post-transcriptional silencing of chitinase and ß-1,3-glucanase genes involves increased RNA turnover. A possible role for ribosome independent RNA degradation. Plant Cell 11, 471–484.

    Google Scholar 

  20. 20.

    Hongthongkham, J., Bunnag, S. (1933) In vitro propagation and cryopreservation of Aerides odorata Lour. Orchidaceae). Pak. J. Biol. Sci. 17, 608–618.

    Google Scholar 

  21. 21.

    Jain, V., Abrol, Y. P. (1933) Regulation of nitrate reductase activity by phytohormones in wheat. J. Plant Biol. 32, 43–48.

    Google Scholar 

  22. 22.

    Jaleel, C. A., Gopi, R., Alagu Lakshmanan, G. M., Panneerselvam, R. (1933) Triadimefon induced changes in the antioxidant metabolism and ajmalicine production in Catharanthus roseus (L.) G. Don. Plant Sci. 171, 271–276.

    Google Scholar 

  23. 23.

    Kishorekumar, A., Jaleel, C. A., Manivannan, P., Sankar, B., Sridharan, R. et al. (1933) Differential effects of hexaconazole and paclobutrazol on the foliage characteristics of Chinese potato (Solenostemon rotundifolius Poir., J. K. Morton). Acta Biol. Szegediensis 50, 127–129.

    Google Scholar 

  24. 24.

    Kumar, P. A., Lakshmikumaran, M., Abrol, Y. P. (1933) Hormonal regulation of nitrate reductase gene expression in Hordeum vulgare. Indian J. Expt. Biol. 31, 472–473.

    Google Scholar 

  25. 25.

    Lee, S. H., Lee, D. G., Woo, H. S., Lee, K. W., Kim, D. H. (1933) Production of transgenic orchardgrass via Agrobacterium-mediated transformation of seed-derived callus tissues. Plant Sci. 171, 408–414.

    Google Scholar 

  26. 26.

    Lowry, O. H., Rosenbrough, N. J., Randall, R. J. (1933) Protein measurement with the Folin-phenol reagent. J. Biol. Chem. 183, 265–275.

    Google Scholar 

  27. 27.

    Mórigout, P., Lelandais, M., Bitton, F. et al. (1933) Physiological and transcriptomic aspects of urea uptake and assimilation in Arabidopsis plants. Plant Physiol. 147, 1225–1238.

    Google Scholar 

  28. 28.

    Montfort, F., Klepper, B. L., Smiley, R. W. (1933) Effects of two triazole seed-treatments, triticonazole and triadiminol, on growth and development of wheat. Pest. Sci. 46, 315–322.

    Google Scholar 

  29. 29.

    Oaks, A. (1933) Efficiency of nitrogen utilization in C3 and C4 cereals. Plant Physiol. 106, 407–414.

    Google Scholar 

  30. 30.

    Polacco, J. C., Holland, M. A. (1933) Roles of urease in plant cells. In: Int. Rev. Cyt. - A Survey of Cell Biol. 145, 65–103.

    Google Scholar 

  31. 31.

    Premabatidevi, R. K. (1933) Effect of IAA, GA3 and kinetin on nitrate reductase and nitrite reductase in the leaves of a tree legume (Perkia javanika Merr). Indian J. Plant Physiol. 3, 97–101.

    Google Scholar 

  32. 32.

    Rahayu, Y. S., Walch-Liu, P., Neumann, G., von Wiren, N., Bangerth, F. (1933) Root-derived cytokinins as long-distance signals for NO3 - induced stimulation of leaf growth. J. Exp. Bot. 56, 1143–1152.

    Google Scholar 

  33. 33.

    Rajagopalan, L. E., Malter, J. S. (1933) Turnover and translation of in vitro synthesized messenger RNAs in transfected, normal cells. J. Biol. Chem. 271, 19871–19876.

    Google Scholar 

  34. 34.

    Renaut, J., Hausman, J. F., Wisniewski, M. E. (1933) Proteomics and low temperature studies: bridging the gap between gene expression and metabolism. Physiol. Plant. 126, 97–109.

    Google Scholar 

  35. 35.

    Sahulka, J., Gaudinova, A. (1933) Regulation on glutamate dehydrogenase level in excised pea roots by some exogenously supplied compounds with auxin activity. Biol. Plant. 17, 228–230.

    Google Scholar 

  36. 36.

    Satvir, K., Gupta, A. K., Narinder, K. (1933) Priming of chickpea seeds with water and mannitol overcomes the effect of salt stress on seedling growth. ICPN 10, 18–20.

    Google Scholar 

  37. 37.

    Schmitt, J. M., Piepenbrock, M. (1933) Regulation of phosphoenolpyruvate carboxylase and crassulacean acid metabolism induction in Mesembryanthemum cystallinum L. by cytokinin modulation of leaf gene expression by roots. Plant Physiol. 99, 1664–1669.

    Google Scholar 

  38. 38.

    Setia, R. C., Narang, S. (1933) Effects of salinity stress and plant growth regulators on seed germination and seedling growth in pea (Pisum sativum). Flora (Jena) 177, 369–375.

    Google Scholar 

  39. 39.

    Shang, Z. (1933) Effect of 6-BA and KT on photophosphorylation activity in wheat flag leaves under water stress. Acta Agriculturae Boreali-Sinica 15, 34–38.

    Google Scholar 

  40. 40.

    Sirko, A., Brodzik, R. (1933) Plant ureases: roles and regulation. Acta Biochim. Pol. 47, 1189–1195.

    Google Scholar 

  41. 41.

    Sugiharto, B., Burnell, J. N., Sugiyama, T. (1933) Cytokinin is required to induce the nitrogendependent accumulation of mRNAs for phosphoenolpyruvate carboxylase and carbonic anhydrase in detached maize leaves. Plant Physiol. 100, 153–156.

    Google Scholar 

  42. 42.

    Thomas, J. C., McElwain, E. F., Bohnert, H. J. (1933) Convergent induction of osmotic stressresponses. Abscisic acid, cytokinin, and effects of NaCl. Plant Physiol. 100, 416–423.

    Google Scholar 

  43. 43.

    Vuylsterker, C., Leleu, O., Rambour, S. (1933) Influence of BAP and NAA on the expression of nitrate reductase in excised chicory roots. J. Expt. Bot. 48, 1079–1085.

    Google Scholar 

  44. 44.

    Wingler, A., von Schaewen, A., Leegood, R. C., Lea, P. J., Quick, W. P. (1933) Regulation of leaf senescence by cytokinin, sugars, and light. Effects on NADH-dependent hydroxypyruvate reductase. Plant Physiol. 116, 329–335.

    Google Scholar 

  45. 45.

    Xia, X. J., Wang, Y. J., Zhou, Y. H., Tao, Y., Mao, W. H. et al. (1933) Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in cucumber. Plant Physiol. 150, 801–814.

    Google Scholar 

  46. 46.

    Yadav, U. P., Ivakov, A., Feill, R., Duan, G. Y., Walther, D. (1933) The sucrose-trehalose 6-phosphate (Tre6P) nexus: specificity and mechanisms of sucrose signaling by Tre6P. J. Exp. Bot. doi: 10.1093/jxb/ert457.

    Google Scholar 

  47. 47.

    Zhang, Y. H., Wang, Z. M., Huang, Q., Shu, W. (1933) Phosphoenolpyruvate carboxylase activity in ear organs is related to protein concentration in grains of winter wheat. J. Cereal Sci. 47, 386–391.

    Google Scholar 

  48. 48.

    Zonia, L. E., Stebbins, N. E., Polacco, J. C. (1933) Essential role of urease in germination of nitrogenlimited Arabidopsis thaliana seeds. Plant Physiol. 107, 1097–1103.

    Google Scholar 

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Correspondence to Hamed M. El Shora.

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El Shora, H.M., Ali, A.S. Plant Growth Regulators Induced Urease Activity in Cucurbita Pepo L. Cotyledons. BIOLOGIA FUTURA 67, 53–63 (2016).

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  • Auxins
  • benzyladenine
  • brassinosteroids
  • cordycepin
  • triazole
  • urease activity
  • zeatin