Study of in Vitro Antimicrobial and Antiproliferative Activities of Selected Saharan Plants

Abstract

The aim of the present study was the evaluation of the antimicrobial and antiproliferative activities of selected Saharan species, which are applied in the traditional medicine but not studied thoroughly from chemical and pharmacological point of view. The studied plants, namely Anthyllis henoniana, Centropodia forskalii, Cornulaca monacantha, Ephedra alata var. alenda, Euphorbia gnyoniana, Helianthemum confertum, Henophyton deserti, Moltkiopsis ciliata and Spartidium saharae were collected from remote areas of North Africa, especially from the Tunisian region of Sahara. After drying and applying the appropriate extraction methods, the plant extracts were tested in antimicrobial screening assay, performed on 19 Gram-positive and -negative strains of microbes. The inhibition zones produced by plant extracts were determined by disc-diffusion method. Remarkable antibacterial activities were exhibited by extracts of Ephedra alata var. alenda and Helianthemum confertum against B. subtilis, M. catarrhalis and methicillin-resistant and non-resistant S. aureus. Minimum inhibitory concentrations of these two species were also determined. Antiproliferative effects of the extracts were evaluated against 4 human adherent cell lines (HeLa, A431, A2780 and MCF7). Notable cell growth inhibition was found for extract of Helianthemum confertum and Euphorbia guy’oniana. Our results provided data for selection of some plant species for further detailed pharmacological and phytochemical examinations.

References

  1. 1.

    Abdallah, L., Chaieb, M. (2007) Water status and growth phenology of a Saharan shrub in North Africa. Afr J. Ecol. 45, 80–85.

    Article  Google Scholar 

  2. 2.

    Ahmed, A. A., Gherraf N., El-Bassuony, A. A., Rhouati, S., Gad, M. H., Ohta, S., Hirata, T. (2006) Guyonianin A and B, 2 polyester diterpenes from Algerian Euphorbia guyoniana. Nat. Prod. Commun. 1, 273–279.

    CAS  Google Scholar 

  3. 3.

    Al-Khalil, S., Alkofahi, A., El-Eisawi, D., Al-Shibib, A. (1998) Transtorine, a new quinoline alkaloid from Ephedra transitoria. J. Nat. Prod. 61, 262–263.

    CAS  Article  Google Scholar 

  4. 4.

    Amar, Z., Labib, S. N., Noureddine, G., Salah, R. (2012) Phytochemical screening of five Algerian plants and the assessment of the antibacterial activity of two extracts Euphorbia guyoniana. Der Pharmacia Lettre. 4, 1438–1444.

    CAS  Google Scholar 

  5. 5.

    Bauer, A. W., Kirby, W. M. M., Sheriss, J. C., Turck, M. (1966) Antibiotic susceptibility testing by standardized single disk method. Am. J. Clin. Pathol. 45, 493–496.

    CAS  Article  Google Scholar 

  6. 6.

    Bouzergoune, F., Bitam, F., Aberkane, M. C., Mosset, P., Fetha, M. N. H., Boudjar, H., Aberkane, A. (2013) Preliminary phytochemical and antimicrobial activity investigations on the aerial parts of Helianthemum kahiricum. Chem. Nat. Compd. 49, 751–752.

    CAS  Article  Google Scholar 

  7. 7.

    Cunningham, P. L. (2013) Plants included in the diet of Arabian Sand Gazelle (Reem) from Saudi Arabia. J. King Saud Univ. - Science 25, 167–173.

    Article  Google Scholar 

  8. 8.

    Derbel, S. (2012) Statut écologique et stratégies adaptatives et fonctionnelles de certaines espèces végétales sahariennes Nord-africaines. Thèse de Doctorat, Université de Sfax, Sfax.

    Google Scholar 

  9. 9.

    Derbel, S., Bouaziz, M., Dhouib, A., Sayadi, S., Chaieb, M. (2010) Chemical composition and biological potential of seed oil and leaf extracts of Henophyton deserti Coss. & Durieu. C. R. Chim. 13, 473–480.

    CAS  Article  Google Scholar 

  10. 10.

    El-Bassuony, A. A. (2007) Antibacterial activity of new polyester diterpenes from Euphorbia guyoniana. Asian J. Chem. 19, 4553–4562.

    CAS  Google Scholar 

  11. 11.

    Ghanem, P. D., Usama, I. A., El-Magly, P. D. (2008) Antimicrobial activity and tentative identification of active compounds from the medicinal Ephedra alataMale plant. J. Taibah. Univ. Med Sci. 3, 7–15.

    Google Scholar 

  12. 12.

    Godevać, D., Zdunić, G., Savikin, K., Vajs, V., Menković, N. (2008) Antioxidant activity of nine Fabaceae species growing in Serbia and Montenegro. Fitoterapia 79, 185–187.

    Article  Google Scholar 

  13. 13.

    Haba, H., Lavaud, C., Marcourt, L., Long, C., Harkat, H., Benkhaled, M. (2009) Ent-abietane diter-penoids from Euphorbia guyoniana Boiss. & Reut. Biochem. Syst. Ecol. 37, 504–508.

    CAS  Article  Google Scholar 

  14. 14.

    Hegazy, M. E. F., Mohamed, A. H., Aoki, N., Ikeuchi, T., Ohta, E., Ohta, S. (2010) Bioactive jat-rophane diterpenes from Euphorbia guyoniana. Phytochemistry 71, 249–253.

    CAS  Article  Google Scholar 

  15. 15.

    Ibrahim, M. (2012) Instinctive Plant Tolerance towards Abiotic Stresses in Arid Regions. In: Najafpour, M. (ed.) Artificial Photosynthesis. In Tech, Rijeka, pp. 219–233.

    Google Scholar 

  16. 16.

    Jeppesen, A. S., Soelberg, J., Jager, A. K. (2012) Antibacterial and COX-1 inhibitory effect of medicinal plants from the Pamir Mountains, Afghanistan Plants 1, 74–81.

    Article  Google Scholar 

  17. 17.

    Kandil, F. E., Grace, M. H. (2001) Polyphenols from Cornulaca monacantha. Phytochemistry 58, 611–613.

    CAS  Article  Google Scholar 

  18. 18.

    Le Houerou, H. N. (1996) Climate change drought & desertification. J. Arid Environ. 34, 133–185.

    Article  Google Scholar 

  19. 19.

    Mann, C. M., Markham, J. L. (1998) A new method for determining the minimum inhibitory concentration of essential oils. J. Appl. Microbiol. 84, 538–544.

    CAS  Article  Google Scholar 

  20. 20.

    Molnár, J., Ocsovszki, I., Puskás, L., Ghane, T., Hohmann, J., Zupkó, I. (2013) Investigation of the antiproliferative action of the quinoline alkaloids kokusaginine and skimmianine on human cell lines. Curr Signal. Transd T. 8, 148–155.

    Article  Google Scholar 

  21. 21.

    Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55–63.

    CAS  Article  Google Scholar 

  22. 22.

    Nawwar, M. A., Barakat, H. H., Buddrus, J., Linscheid, M. (1985) Alkaloidal, lignan and phenolic constituents of Ephedra alata. Phytochemistry 24, 878–879.

    CAS  Article  Google Scholar 

  23. 23.

    Nawwar, M. A., El-Sissi, H., Barakat, H. H. (1984) Flavonoid constituents of Ephedra alata. Phytochemistry 23, 2937–2939.

    CAS  Article  Google Scholar 

  24. 24.

    Ourcival, J. M., Floret, C., Le Floc’h, E., Pontanier, R. (1994) Water relations between two perennial species in the steppes of southern Tunisia. J. Arid Environ. 28, 333–350.

    Article  Google Scholar 

  25. 25.

    Papaefthimiou, D., Papanikolaou, A., Givanoudi, S., Kanellis, A. K., Falara, V., Kostas, S. (2014) Genus Cistus: a model for exploring labdane-type diterpenes’ biosynthesis and a natural source of high value products with biological, aromatic, and pharmacological properties. Front. Chem. 2, 35.

    Article  Google Scholar 

  26. 26.

    Rizk, A. M., Hammouda, F. M., Ismail, S. I., Hassan, N. M., Hosseiny, H. A., Roeder, E., Wiedenfeld, H., Ghaleb, H. A., Madkour, M. K. (1988) Constituents of plants growing in Qatar. Part XV. Chemical investigation and pharmacotoxicity of pyrrolizidine alkaloids of Moltikiopsis ciliata. Int. J. Crude. Drug. Res. 26, 112–116.

    CAS  Article  Google Scholar 

  27. 27.

    Rizk, A. M., Heiba, H. I., Ma’Ayergi, H. A., Batanouny, K. H. (1986) Constituents of plants growing in Qatar. Fitoterapia 57, 3–9.

    CAS  Google Scholar 

  28. 28.

    Rubio-Moraga, A., Argandona, J., Mota, B., Perez, J., Verde, A., Fajardo, J., Gomez-Navarro, J., Castillo-Lopez, R., Ahrazem, O., Gomez-Gomez, L. (2013) Screening for polyphenols, antioxidant and antimicrobial activities of extracts from eleven Helianthemum taxa (Cistaceae) used in folk medicine in south-eastern Spain. J. Ethnopharmacol. 148, 287–296.

    CAS  Article  Google Scholar 

  29. 29.

    Tusevski, O., Kostovska, A., Iloska, A., Trajkovska, L., Simic, S. G. (2014) Phenolic production and antioxidant properties of some Macedonian medicinal plants. Cent. Eur. J. Biol. 9, 888–990.

    CAS  Google Scholar 

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Correspondence to Judit Hohmann.

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Palici, I.F., Liktor-Busa, E., Zupkó, I. et al. Study of in Vitro Antimicrobial and Antiproliferative Activities of Selected Saharan Plants. BIOLOGIA FUTURA 66, 385–394 (2015). https://doi.org/10.1556/018.66.2015.4.3

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Keywords

  • Saharan plants
  • Ephedra alata var. alenda
  • Helianthemum confertum
  • antibacterial activity
  • antiproliferative activity