First Phytochemical Investigation of Secondary Metabolites of Euphorbia Davidii Subils. And Antiproliferative Activity of its Extracts

Abstract

The present work is the first phytochemical investigation of Euphorbia davidii Subils. After multistep separation process, three flavonoid glycosides were obtained from the ethyl acetate soluble fraction of the methanol extract of the whole plant. The structures of the isolated compounds were determined as kaemp-ferol 3-O-rhamnoside, myricetin 3-O-rhamnoside, and quercetin 3-O-rhamnoside. Aqueous and organic extracts of the plant were screened in vitro for antiproliferative activity against HeLa (cervix epithelial adenocarcinoma), A431 (skin epidermoid carcinoma), A2780 (ovarian carcinoma) and MCF7 (breast epithelial adenocarcinoma) cells, using the MTT assay. n-Hexane and chloroform extracts demonstrated moderately dose-dependent cell growth inhibitory activity against all four cell lines.

References

  1. 1.

    Barina, Z., Shevera, M., Sirbu, C., Pinke, G. (2013) Current distribution and spreading of Euphorbia davidii (E. dentata agg.) in Europe. Cent. Eur. J. Biol. 8, 87–95.

    Google Scholar 

  2. 2.

    Jassbi, A. R. (2006) Chemistry and biological activity of secondary metabolites in Euphorbia from Iran. Phytochemistry 67, 1977–1984.

    CAS  Article  Google Scholar 

  3. 3.

    Lage, H., Duarte, N., Coburger, C., Hilgeroth, A., Ferreira, M. J. U. (2010) Antitumor activity of terpenoids against classical and atypical multidrug resistant cancer cells. Phytomedicine 17, 441–448.

    CAS  Article  Google Scholar 

  4. 4.

    Markham, K. R., Ternai, B., Stanley, R., Geiger, H., Mabry, T. J. (1978) Carbon-13 NMR studies of flavonoids—III: Naturally occurring flavonoid glycosides and their acylated derivatives. Tetrahedron 34, 1389–1397.

    CAS  Article  Google Scholar 

  5. 5.

    Markham, K. R., Geiger, H. (1994) ’H nuclear magnetic resonance spectroscopy of flavonoids and their glycosydes in hexadeuterodimethylsulfoxide. In: Harborne, J. B. (ed.) The Flavonoids. Chapman & Hall, London, pp. 441–497.

    Google Scholar 

  6. 6.

    Mok, S. Y., Lee, S. (2013) Identification of flavonoids and flavonoid rhamnosides from Rhododendron mucronulatum for. albiflorum and their inhibitory activities against aldose reductase. Food Chem. 136, 969–974.

    CAS  Article  Google Scholar 

  7. 7.

    Molnar, J., Ocsovszki, I., Puskas, L., Ghane, T., Hohmann, J., Zupko, I. (2013) Investigation of the antiproliferative action of the quinoline alkaloids kokusaginine and skimmianine on human cell lines. Curr Signal Transduct. Ther 8, 148–155.

    CAS  Article  Google Scholar 

  8. 8.

    Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55–63.

    CAS  Article  Google Scholar 

  9. 9.

    Schultes, R. E. (1987) Members of the Euphorbiaceae in primitive and advanced societies. Bot. J. Linn. Soc. 94, 79–95.

    Article  Google Scholar 

  10. 10.

    Strack, D., Heilmann, J., Momken, M., Wray, V. (1988) Cell wall-conjugated phenolics from Coniferae leaves. Phytochemistry 27, 3517–3521.

    CAS  Article  Google Scholar 

  11. 11.

    Vasas, A., Redei, D., Csupor, D., Molnar, J., Hohmann, J. (2012) Diterpenes from European Euphorbia species serving as prototypes for natural-product-based drug discovery. Eur. J. Org. Chem. 2012, 5115–5130.

    CAS  Article  Google Scholar 

  12. 12.

    Yang, Y., Riina, R., Morawetz, J. J., Haevermans, T., Aubriot, X., Berry, P. E. (2012) Molecular phylogenetics and classification of Euphorbia subgenus Chamaesyce (Euphorbiaceae). Taxon 61, 764–789.

    Article  Google Scholar 

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Correspondence to Dóra Rédei.

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Rédei, D., Kúsz, N., Szabó, M. et al. First Phytochemical Investigation of Secondary Metabolites of Euphorbia Davidii Subils. And Antiproliferative Activity of its Extracts. BIOLOGIA FUTURA 66, 464–467 (2015). https://doi.org/10.1556/018.66.2015.4.11

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Keywords

  • Euphorbia davidii
  • flavonoid glycoside
  • antiproliferative activity