Evaluation of Polyphenol Composition and Biological Activities of Two Samples from Summer and Winter Seasons of Ligularia Fischeri Var. Spiciformis Nakai


Phenolic contents, antioxidant and antimicrobial activities were determined by two samples from summer (June) and winter (December) seasons of Ligularia fischeri var. spiciformis Nakai. A total of 24 phenolic compounds were identified by ultra-performance liquid chromatography (UPLC) analysis. Myricetin (1964.35 and 1829.12 µg/g) was the most dominant flavonol compared to quercetin and kaempferol. Salicylic acid (222.80 and 215.25 μg/g) was the most important phenolic compound compared to pyrogallol, caffeic acid, gentisic acid, o-coumaric acid, gallic acid, protocatechuic acid and ferulic acid in summer (June) and winter (December) seasons. Phenolic contents and antioxidant capacities were estimated for the various solvent extracts (petroleum ether, butanol, ethyl acetate, methanol and water). Ethyl acetate extract exhibited the highest phenolic (332.64 and 299.44 mg/g gallic acid equivalent) and flavonoid contents (5.72 and 5.29 mg/g quercetin equivalent) and also the strongest antioxidant activity in summer and winter seasons. Due to these metabolic variations, the antioxidant and antimicrobial activities were increased with summer seasons compared to winter seasons. Our study shows that the samples collected in June had higher phenolic compounds, stronger antioxidative and antimicrobial activity than the samples of L. fischeri leaf extracts collected in December.


  1. 1.

    Bae, J. H., Yu, S. O., Kim, Y. M., Chon, S. U., Kim, B. W., Heo, B. G. (2009) Physiological activity of methanol extracts from Ligularia fischeri and their hyperplasia inhibition activity of cancer cell. J. Biol. Environ. Cont. 18, 67–73.

    Google Scholar 

  2. 2.

    Boyle, S. P., Dobson, V. L., Duthie, S. J., Kyle, J. A., Collins, A. R. (2000) Absorption and DNA protective effects of flavonoid glycosides from an onion meal. Euro. J. Nutr. 39, 213–223.

    CAS  Article  Google Scholar 

  3. 3.

    Carvalho, I. S., Cavaco, T., Brodelius, M. (2011) Phenolic composition and antioxidant capacity of six Artemisia species. Industrial Crops Prod. 33, 382–388.

    CAS  Article  Google Scholar 

  4. 4.

    Choi, E. M., Ding, Y., Nguyen, H. T., Park, S. H., Kim, Y. H. (2007) Antioxidant activity of gomchi (Ligularia fischeri) leaves. Food Sci. Biotechnol. 16, 710–714.

    Google Scholar 

  5. 5.

    Choi, E. M., Kim, Y. H. A. (2008) Preliminary study of the effects of an extract of Ligularia fischeri leaves on type II collagen-induced arthritis in DBA/1J mice. Food Chem. Toxicol. 46, 375–379.

    CAS  Article  Google Scholar 

  6. 6.

    Elzaawely, A. A., Xuan, T. D., Tawata, S. (2005) Antioxidant and antibacterial activities of Rumex japonicus HOUTT. aerial parts. Biol. Pharm. Bull. 28, 2225–2230.

    CAS  Article  Google Scholar 

  7. 7.

    Ham, S. S., Lee, S. Y., Oh, D. H., Jung, S. W., Kim, S. H., Jeong, C. H., Kang, I. J. (1998) Cytotoxicity of Ligularia fischeri extracts. Kor. Soc. Food Sci. Nutr. 27, 987–992.

    Google Scholar 

  8. 8.

    Hilton, P. J., Palmer-Jones, R., Ellis, R. T. (1973) Effects of season and nitrogen fertilizer upon the flavanol composition and tea making quality of fresh shoots of tea (Camellia sinensis L.) in central Africa. J. Sci. Food Agri. 24, 819–826.

    CAS  Article  Google Scholar 

  9. 9.

    Janssen, K., Mensink, R., Cox, F., Harryvan, J., Hovenior, R., Hollman, P., Katan, M. (1998) Effects of the flavonoids quercetin and apigenin on hemostasis in healthy volunteers: results from an in vitro and a dietary supplement study. Am. J. Clinic. Nutr. 2, 255–262.

    Article  Google Scholar 

  10. 10.

    Jayanthi, P., Lalitha, P. (2011) Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. Int. J. Pharm. Pharmaceutical Sci. 3, 126–128.

    Google Scholar 

  11. 11.

    Jeong, S. W., Kim, E. J., Hwangbo, H. J., Ham, S. S. (1998) Effects of Ligularia fischeri extracts on oxidation of low density lipoprotein. Kor. J. Food Sci. Tech. 30, 1214–1221.

    Google Scholar 

  12. 12.

    Kim, S. M., Jeon, J. S., Kang, S. W., Jung, Y. J., Ly, L. N., Um, B. H. (2012) Content of antioxidative caffeoylquinic acid derivatives in field-grown Ligularia fischeri (Ledeb.) Turcz and responses to sunlight. J. Agric. Food Chem. 60, 5597−5603.

    CAS  Article  Google Scholar 

  13. 13.

    Lee, K. T., Koo, S. J., Jung, S. H., Choi, J. W., Jung, H. J., Park, H. J. (2002) Structure of three new terpenoids, spiciformisins a and b, and monocyclosqualene, isolated from the herbs of Ligularia fischeri var. spiciformis and cytotoxicity. Arch. Pharm. Res. 25, 820–823.

    CAS  Article  Google Scholar 

  14. 14.

    Lee, Y. J., Thiruvengadam, M., Chung, I. M., Nagella, P. (2013) Polyphenol composition and antioxidant activity from the vegetable plant Artemisia absinthium L. Aust. J. Crop Sci. 7, 1921–1926.

    CAS  Google Scholar 

  15. 15.

    Nagella, P., Thiruvengadam, M., Ahmad, A., Chung, I. M. (2014) UHPLC analysis of polyphenol composition and antioxidant activity from different solvent extracts of Coriandrum sativum seeds cultivated in Korea. Asian J. Chem. 26, 6351–6356.

    CAS  Article  Google Scholar 

  16. 16.

    Pai, S. R., Masingraj, S., Nimbalkar, Pawar, N. V., Patil, R. P., Dixit, G. B. (2010) Seasonal discrepancy in phenolic content and antioxidant properties from bark of Nothapodytes nimmoniana (Grah.) Mabb. Int. J. Pharma Bio Sci. 1, 1–17.

    Google Scholar 

  17. 17.

    Pattison, D. J., Silman, A. J., Goodson, N. J., Lunt, M., Bunn, D., Luben, R., Welch, A., Bingham, S., Khaw, K. T., Day, N., Symmons, D. P. (2004) Vitamin C and the risk of developing inflammatory polyarthritis: Prospective nested case-control study. Ann. Rheumatic Diseases 63, 843–847.

    CAS  Article  Google Scholar 

  18. 18.

    Ross, J., Kasum, C. (2002) Dietary flavonoids: Bioavailability, metabolic effects, and safety. Ann. Rev. Nutr. 22, 19–34.

    CAS  Article  Google Scholar 

  19. 19.

    Thiruvengadam, M., Chung, I. M. (2015) Selenium, putrescine, and cadmium influence health-promoting phytochemicals and molecular-level effects on turnip (Brassica rapa ssp. rapa). Food Chem. 173, 185–193.

    CAS  Article  Google Scholar 

  20. 20.

    Thiruvengadam, M., Praveen, N., Maria John, K. M., Yang, Y. S., Kim, S. H., Chung, I. M. (2014a) Establishment of Momordica charantia hairy root cultures for the production of phenolic compounds and determination of their biological activities. Plant Cell Tissue Org. Cult. 118, 545–557.

    CAS  Article  Google Scholar 

  21. 21.

    Thiruvengadam, M., Praveen, N., Yu, B. R., Kim, S. H., Chung, I. M. (2014b) Polyphenol composition and antioxidant capacity from different extracts of Aster scaber. Acta Biol. Hung. 65, 144–155.

    CAS  Article  Google Scholar 

  22. 22.

    Waladkhani, A., Clemens, M. R. (1998) Effect of dietary phytochemicals on cancer development. International J. Molecular Med. 1, 747–753.

    CAS  Google Scholar 

  23. 23.

    Wong, P. Y. Y., Kitts, D. D. (2006) Studies on the dual antioxidant and antibacterial properties of parsley (Petroselinum crispum) and cilantro (Coriandrum sativum) extracts. Food Chem. 97, 505–515.

    CAS  Article  Google Scholar 

  24. 24.

    Yao, L., Caffin, N., D’Arcy, B., Jiang, Y., Shi, J., Sinaganusong, R., Liu, X., Datta, N., Kakuda, Y., Xu, Y. (2005) Seasonal variations of phenolic compounds in Australia-grown tea (Camellia sinensis). J. Agric. Food Chem. 53, 6477–6483.

    CAS  Article  Google Scholar 

  25. 25.

    Zhang, W. J., Qi, H. Y., Shi, Y. P. (2010) Norsesquiterpene derivatives from the roots of Ligularia fischeri. Planta Med. 76, 159–164.

    CAS  Article  Google Scholar 

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Correspondence to Muthu Thiruvengadam.

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Rekha, K., Sivasubramanian, C. & Thiruvengadam, M. Evaluation of Polyphenol Composition and Biological Activities of Two Samples from Summer and Winter Seasons of Ligularia Fischeri Var. Spiciformis Nakai. BIOLOGIA FUTURA 66, 179–191 (2015). https://doi.org/10.1556/018.66.2015.2.5

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  • Biological activities
  • phenolic compounds
  • flavonoids
  • seasonal variations
  • Ligularia fischeri