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Bioremediation of PCB-contaminated sediments and evaluation of their pre- and post-treatment ecotoxicity

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Abstract

The paper deals with the application of two perspective and promising bioremediation approaches, bioaugmentation and biostimulation, applied to sediment contaminated with polychlo-rinated biphenyls (PCBs) with the aim to enhance biodegradation of PCBs. Sediments were sampled from Strážsky canal, an industrial waste canal that flows from a former PCB-factory through the Laborec river into the Zemplínska šírava water reservoir, all located in the eastern part of Slovakia. Bioaugmentation of sediments was performed in microcosms using two bacterial isolates with PCB-degradation ability obtained from the contaminated sediment: Achromobacter xylosoxidans, Stenotrophomonas maltophilia. Biostimulation was performed using an addition of cut plants containing terpenes, known as PCB-inducers (ivy leaves and pine needles). Ecotoxicity of the contaminated sediments was evaluated pre- and post-treatment using biotests of the standard aquatic plant Lemna minor, standard contact test using Lactuca sativa var. capitata, and the bioluminescent bacteria Vibrio fischeri. Biostimulation treatment using ivy leaves revealed higher degradation of detected PCB congeners than that achieved by the addition of pine needles, but moderately higher post-treatment toxicity of the sediment to the bioindicator Lemna minor.

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References

  • Anyasi, R. O., Atagana, H. I. (2011). Biological rermediation of polychlorinated biphenyls (PCBs) in the environment by microorganisms and plants. African Journal of Biotechnology, 10, 18916–18938. DOI: 10.5897/ajb10.557.

    Article  CAS  Google Scholar 

  • Apitz, S. E., Brils, J., Marcomini, A., Agostini, P., Micheletti, C., Pippa, R., Scanferla, P., Zuin, S., Lánczos, T., Dercová, K., Kocan, A., Petrík, J., Hucko, P., Kušnír, P. (2006). Approaches and frameworks for managing contaminated sediments–A European Perspective. In D. Reible, S. Apitz (Eds.), Assessment and remediation of contaminated sediments, Chapter 1. Serie IV: Earth &Environmental Science 19 (pp. 5–82). New York, NY, USA: Springer. DOI: 10.1007/978-1-4020-4959-0.

    Chapter  Google Scholar 

  • Bedard, D. (2003). Polychlorinated biphenyls in aquatic sediments: Environmental fate and outlook for biological treatment. In M. M. Häggblom, I. D. Bossert (Eds.), Dehalo-genation: Microbial processes and environmental application (pp. 443–465). Boston, MA, USA: Kluwer Academic Publishers. DOI: 10.1007/978-0-0306-48011-9.

    Google Scholar 

  • Borja, J. Q., Auresenia, J. L., & Gallardo, S. M. (2006). Biodegradation of polychlorinated biphenyls using biofilm grown with biphenyl as carbon source in fluidized bed reactor. Chemosphere, 64, 555–559. DOI: 10.1016/j.chemosphere. 2005.11.047.

    Article  CAS  Google Scholar 

  • Chen, F., Hao, S., Qu, J., Ma, J., Zhang, S. (2015). Enhanced biodegradation of polychlorinated biphenyls by defined bacteria-yeast consortium. Annals of Microbiology, 65, 1847–1854. DOI: 10.1007/s.13213-014-1023-8.

    Article  CAS  Google Scholar 

  • Conka, K., Chovancová, J., Stachová Sejáková, Z., Dömötörová, M., Fabišiková, A., Drobná, B., Kocan, A. (2014). PCDDs, PCDFs, and OCPs in sediments from selected areas in the Slovak Republic. Chemosphere, 98, 37–43. DOI: 10.1016/j.chemosphere.2013.09.068.

    Article  CAS  Google Scholar 

  • Demnerová, K., Lovecká, P., Mat?eju, L., Macková, M., Filip, A. Z. (2007). Approaches in environmental ecotoxicology. In P. P. Simeonova, N. Opopol, M. I. Luster (Eds.), Nanotechnology–toxicological issues and environmental safety (pp. 249–270). New York, NY, USA: Springer. DOI: 10.1007/978-1-4020-6076-2.

    Chapter  Google Scholar 

  • Dercová, K., Šeligová, J., Dudášová, H., Mikulášová, M., Šil-hárová, K., Tóthová, L., Hucko, P. (2009). Characterization of the bottom sediments contaminated with polychlo-rinated biphenyls: Evaluation of ecotoxicity and biodegrad-ability. International Biodeterioration & Biodegradation, 63, 440–449. DOI: 10.1016/j.ibiod.2008.12.005.

    Article  Google Scholar 

  • Dudášová, H., Lukácová, L., Murínová, S., Dercová, K. (2012). Effects of plant terpenes on biodegradation of poly-chlorinated biphenyls (PCBs). International Biodeterioration &Biodegradation, 69, 23–27. DOI: 10.1016/j.ibiod.2012.01.003.

    Article  Google Scholar 

  • Dudášová, H., Lukácová, L., Murínová, S., Puškárová, A., Pan-gallo, D., Dercová, K. (2014). Bacterial strains isolated from PCB-contaminated sediments and their use for bioaugmentation strategy in microcosms. Journal of Basic Microbiology, 54, 253–260. DOI: 10.1002/jobm.201200369.

    Article  Google Scholar 

  • Egorova, D. O., Demakov, V. A., Plotnikova, E. G. (2013). Bioaugmentation of a polychlorobiphenyl contaminated soil with two bacterial strains. Journal of Hazardous Materials, 261, 378–386. DOI: 10.1016/j.jhazmat.2013.07.067.

    Article  CAS  Google Scholar 

  • Fagervold, S. K., Watts, J. E. M., May, H. D., Sowers, K. R. (2011). Effects of bioaugmentation on indigenous PCB dechlorinating activity in sediment microcosms. Water Research, 45, 3899–3907. DOI: 10.1016/j.watres.2011.04.048.

    Article  CAS  Google Scholar 

  • Field, J. A., Sierra-Alvarez, R. (2008). Microbial transformation and degradation of polychlorinated biphenyls. Environmental Pollution, 155, 1–12. DOI: 10.1016/j.envpol.2007.10. 016.

    Article  CAS  Google Scholar 

  • Hernandéz, B. S., Koh, S. C., Chial, M., Focht, D. D. (1997). Terpene utilizing isolates and their relevance to enhanced biotransformation of polychlorinated biphenyls in soil. Biodegradation, 8, 153–158. DOI: 10.1023/a:100825521 8432.

    Article  Google Scholar 

  • Hiller, E., Zemanová, L., Sirotiak, M., Jurkovic, L. (2011). Concentrations, distributions, and sources of polychlori-nated biphenyls and polycyclic aromatic hydrocarbons in bed sediments of the water reservoirs in Slovakia. Environmental Monitoring and Assessment, 173, 883–897. DOI: 10.1007/s10661-010-1431-6.

    Article  CAS  Google Scholar 

  • International Organization for Standardization (2006). Water quality–Sampling–Part 1: Guidance on the design of sampling programmes and sampling techniques. ISO 5667-12:2006. Geneva, Switzerland.

    Google Scholar 

  • Jung, K. J., Kim, E., So, J. S., Koh, S. C. (2001). Specific biodegradation of polychlorinated biphenyls (PCBs) facilitated by plant terpenes. Biotechnology and Bioprocess Engineering, 6, 61–66. DOI: 10.1007/bf02942252.

    Article  CAS  Google Scholar 

  • Jung, K. J., Kim, B. H., Kim, E., So, J. S., Koh, S. C. (2002). Monitoring expression of bphC gene from Ralstonia eutropha H850 induced by plant terpenes in soil. Journal of Microbiology, 40, 340–343.

    CAS  Google Scholar 

  • Kocan, A., Petrik, J., Jursa, S., Chovancova, J., Drobna, B. (2001). Environmental contamination with polychlori-nated biphenyls in the area of their former manufacture in Slovakia. Chemosphere, 43, 595–600. DOI: 10.1016/s0045-6535(00)00411-2.

    Article  CAS  Google Scholar 

  • Koubek, J., Mackova, M., Macek, T., & Uhlik, O. (2013). Diversity of chlorobiphenyl-metabolizing bacteria and their biphenyl dioxygenases in contaminated sediment. Chemo-sphere, 93, 1548–1555. DOI: 10.1016/j.chemosphere.2013.07. 073.

    Article  CAS  Google Scholar 

  • Kwon, S. H., Hong, M. H., Choi, J. H., Whang, K. S., Lee, H. S., So, J. S., Koh, S. C. (2008). Bioremediation of Aroclor 1242 by a consortium culture in marine sediment microcosms. Biotechnology and Bioprocess Engineering, 13, 730–737. DOI: 10.1007/s12257-008-0111-7.

    Article  CAS  Google Scholar 

  • Lebeau, T. (2011). Bioaugmentation for in situ soil remediation: How to ensure the success of such a process. In A. Singh, N. Parmar, R. C. Kuhad, (Eds.), Bioaugmentation, biostimulation and biocontrol, soil biology (Vol. 28 pp. 129–186). New York, NY, USA: Springer. DOI: 10.1007/978-3-642-19769-7 2.

    Article  Google Scholar 

  • Luo, W., D’Angelo, E. M., Coyne, M. S. (2008). Organic carbon effects on aerobic polychlorinated biphenyl removal and bacterial community composition in soils and sediments. Chemosphere, 70, 364–373. DOI: 10.1016/j.chemosphere. 2007.07.022.

    Article  CAS  Google Scholar 

  • Mackova, M., Uhlik, O., Lovecka, P., Viktorova, J., Novakova, M., Demnerova, K., Sylvestre, M., Macek, T. (2010). Bacterial degradation of polychlorinated biphenyls. Geomicrobiology: Molecular and Environmental Perspective, 2010, 347–366. DOI: 10.1007/978-90-481-9204-5 16.

    Google Scholar 

  • Murínová, S., Dercová, K., & Dudášová, H. (2014). Degradation of polychlorinated biphenyls (PCBs) by four bacterial isolates from the PCB-contaminated soil and PCB-contaminated sediment. International Biodeterioration &Biodegradation, 91, 52–59. DOI: 10.1016/j.ibiod.2014.03.011.

    Article  Google Scholar 

  • Oh, E. T., Koh, S. C., Kim, E., Ahn, Y. H., So, J. S. (2003). Plant terpenes enhance survivability of polychlori-nated biphenyl (PCB) degrading Pseudomonas pseudoalcali-genes KF707 labeled with gfp in microcosms contaminated with PCB. Journal of Microbiology and Biotechnology, 13, 463–468.

    CAS  Google Scholar 

  • Perelo, L. W. (2011). In situ bioremediation of organic pollutants in aquatic sediments. Journal of Hazardous Materials, 177, 81–89. DOI: 10.1016/j.jhazmat.2009.12.090.

    Article  Google Scholar 

  • Petríc, I., Hršak, D., Fingler, S., Udikovíc-Kolíc, N., Bru, D., Martin-Laurent, F. (2011). Insight in the PCB–degrading functional community in long-term contaminated soil under bioremediation. Journal of Soils and Sediments, 11, 290–300. DOI: 10.1007/s11368-010-0299-y.

    Article  Google Scholar 

  • Rhodes, A. H., Owen, S. M., & Semple, K. T. (2007). Biodegradation of 2,4-dichlorophenol in the presence of volatile organic compounds in soils under different vegetation types. FEMS Microbiology Letters, 269, 323–330. DOI: 10.1111/j.1574-6968.2007.00657.x.

    Article  CAS  Google Scholar 

  • Slovak Environmental Agency (1998). Slovak standard: Metod-icky pokyn na hodnotenie rizík zo znecistených sedimentov tokov a vodných nádrží. No. 549/98-2. Bratislava, Slovakia.

    Google Scholar 

  • Slovak Office of Standards, Metrology and Testing (1996). Slovak standard: Water quality. Determination of certain organochlorine insecticides, polychlorinated biphenyls and chlorobenzenes–Gas chromatographic method after liquidliquid extraction. STN ISO 6468:1996. Bratislava, Slovakia.

    Google Scholar 

  • Slovak Office of Standards, Metrology and Testing (2006). Slovak standard: Water quality. Sampling. Part 12: Guidance on the design of sampling programmes and sampling techniques (757051). STN ISO 5667-12:2006. Bratislava, Slovakia.

    Google Scholar 

  • Slovak Office of Standards, Metrology and Testing (2007). Slovak standard: Water quality. Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test). Part 2: Method using liquid-dried bacteria 757445-2007. STN ISO 11348-2:2007. Bratislava, Slovakia.

    Google Scholar 

  • Slovak Office of Standards, Metrology and Testing (2008). Slovak standard: Water quality (2008). Determination of the toxic effect of water constituents and waste water to duckweed (Lemna minor)–Duckweed growth inhibition test. (757747). STN ISO 20079. Bratislava, Slovakia.

    Google Scholar 

  • Tandlich, R., Brežná, B., Dercová, K. (2001). The effect of terpenes on the biodegradation of polychlorinated biphenyls by Pseudomonas stutzeri. Chemosphere, 44, 1547–1555. DOI: 10.1016/s0045-6535(00)00523-3.

    Article  CAS  Google Scholar 

  • United States Environmental Protection Agency (2007). US standard: Organochlorine pesticides by gas chromatography. US EPA Method 8089/8081. Dallas, TX, USA.

    Google Scholar 

  • Varadhan, A. S., Khodadoust, A. P., Brenner, R. C. (2011). Effect of biostimulation on the microbial community in PCB-contaminated sediments through periodic amendment of sediment with iron. Journal of Industrial Microbiology & Biotechnology, 38, 1691–1707. DOI: 10.1007/s10295-011-0959-y.

    Article  Google Scholar 

  • Vasilyeva, G. K., & Strijakova, E. R. (2007). Bioremedia-tion of soils and sediments contaminated by polychlorinated biphenyls. Microbiology, 76, 639–653. DOI: 10.1134/s002626 170706001x.

    Article  CAS  Google Scholar 

  • Villeneuve, J. P., Carvalho, F. P., Fowler, S. W., Cattini, C. (1999). Levels and trends of PCBs, chlorinated pesticides and petroleum hydrocarbons in mussels from the NW Mediterranean coast: comparison of concentrations in 1973/1974 and 1988/1989. Science of the Total Environment, 237–238, 57–65. DOI: 10.1016/s0048-9697(99)00124-2.

    Article  Google Scholar 

  • Wiegel, J., Wu, Q. Z. (2000). Microbial reductive dehalogena-tion of polychlorinated biphenyls. FEMS Microbiol Ecology, 32, 1–15. DOI: 10.1111/j.1574-6941.2000.tb00693.x.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemical Technology, Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37, Bratislava, Slovakia

    Hana Dudášová, Katarína Lászlová, Lucia Lukáčová, Marta Balaščáková & Katarína Dercová

  2. Water Research Institute, Nábrežie arm. gen. L. Svobodu 5, 812 49, Bratislava, Slovakia

    Slavomíra Murínová

Authors
  1. Hana Dudášová
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  2. Katarína Lászlová
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  3. Lucia Lukáčová
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  4. Marta Balaščáková
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  5. Slavomíra Murínová
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  6. Katarína Dercová
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Correspondence to Hana Dudášová.

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Dudášová, H., Lászlová, K., Lukáčová, L. et al. Bioremediation of PCB-contaminated sediments and evaluation of their pre- and post-treatment ecotoxicity. Chem. Pap. 70, 1049–1058 (2016). https://doi.org/10.1515/chempap-2016-0041

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