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Fabrication of amperometric cholesterol biosensor based on SnO2 nanoparticles and Nafion-modified carbon paste electrode

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Abstract

This study reports the fabrication of an amperometric cholesterol biosensor based on cholesterol oxidase (ChOx), SnO2NPs and Nafion-modified carbon paste enzyme electrodes (CPE/SnO2NPs-ChOx/Naf). The electrochemical characterisations of BCPE and CPE/SnO2NPs were performed using CV and EIS. The determination of cholesterol was carried out by electrochemical oxidation of H2O2 at 0.6 V vs. Ag/AgCl. The CPE/SnO2NPs-ChOx/Naf presented a linear range from 0.20 μ.mol L−1 to 4.95 μmol L−1 with a low limit of detection (0.04 μ.mol L−1). In addition, the optimal values for pH and temperature were found to be 7.5 and 35°C, respectively. The CPE/SnO2NPs-ChOx/Naf was used for the determination of cholesterol in serum samples and good results were obtained.

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  1. Department of Chemistry, Faculty of Science, Ankara University, Ankara, 06100, Turkey, USA

    Gözde Aydoğdu Tığ & Şule Pekyardımcı

  2. Department of Biochemistry, Faculty of Science and Arts, Dumlupinar University, Kütahya 43100, Turkey, USA

    Derya Koyuncu Zeybek

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  1. Gözde Aydoğdu Tığ
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Tığ, G.A., Zeybek, D.K. & Pekyardımcı, Ş. Fabrication of amperometric cholesterol biosensor based on SnO2 nanoparticles and Nafion-modified carbon paste electrode. Chem. Pap. 70, 695–705 (2016). https://doi.org/10.1515/chempap-2016-0005

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