Skip to main content
Log in

Application of polypyrrole nanowires for the development of a tyrosinase biosensor

  • Short Communication
  • Published:
Chemical Papers Aims and scope Submit manuscript

Abstract

Polypyrrole nanowires (PPyNWs) were fabricated and examined as a structural component of amperometric biosensor matrix. An enzyme, tyrosinase (TYR), was immobilized onto PPyNWs using glutaraldehyde (GA). Matrix composite morphology was investigated using scanning electron microscopy. Electrochemical behavior of the prepared PPyNWs/GA/TYR biosensor towards catechol was studied and the assessment of its analytical characteristics was carried out taking into account linear range, sensitivity, repeatability, reproducibility and operational stability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Apetrei, C., Rodríguez-Méndez, M. L., & De Saja, J. A. (2011). Amperometric tyrosinase based biosensor using an electropolymerized phosphate-doped polypyrrole film as an immobilization support. Application for detection of phenolic compounds. Electrochimica Acta, 56, 8919–8925. DOI: 10.1016/j.electacta.2011.07.127.

    Article  CAS  Google Scholar 

  • Bai, S. L., Zhang, K. W., Sun, J. H., Zhang, D. F., Luo, R. X., Li, D. Q., & Liu, C. C. (2014). Polythiophene—WO3 hybrid architectures for low-temperature H2S detection. Sensors and Actuators B, 197, 142–148. DOI: 10.1016/j.snb.2014.02.038.

    Article  CAS  Google Scholar 

  • Cernat, A., Le Goff, A., Holzinger, M., Sandulescu, R., & Cosnier, S. (2014). Micro- to nanostructured poly(pyrrolenitrilotriacetic acid) films via nanosphere templates: Applications to 3D enzyme attachment by affinity interactions. Analytical and Bioanalytical Chemistry, 406, 1141–1147. DOI: 10.1007/s00216-013-7135-3.

    Article  CAS  Google Scholar 

  • Ćirić-Marjanović, G., Pašti I., Gavrilov, N., Janošević, A., & Mentus, S. (2013). Carbonised polyaniline and polypyrrole: Towards advanced nitrogen-containing carbon materials. Chemical Papers, 67, 781–813. DOI: 10.2478/s11696-013-0312-1.

    Google Scholar 

  • ElKaoutit, M., Naranjo-Rodriguez, I., Domínguez, M., & Hidalgo-Hidalgo-de-Cisneros, J. L. (2011). Bio-functionalization of electro-synthesized polypyrrole surface by heme enzyme using a mixture of Nafion and glutaraldehyde as synergetic immobilization matrix: Conformational characterization and electrocatalytic studies. Applied Surface Science, 257, 10926–10935. DOI: 10.1016/j.apsusc.2011.08.00.

    Article  CAS  Google Scholar 

  • Hnida, K. E., Socha, R. P., & Sulka, G. D. (2013). Polypyrrole-silver composite nanowire arrays by cathodic co-deposition and their electrochemical properties. The Journal of Physical Chemistry C, 117, 19382–19392. DOI: 10.1021/jp4038304.

    CAS  Google Scholar 

  • Hamilton, A., & Breslin, C. B. (2014). The development of a novel urea sensor using polypyrrole. Electrochimica Acta, 145, 19–26. DOI: 10.1016/j.electacta.2014.08.052.

    Article  CAS  Google Scholar 

  • Han, R. X., Cui, L., Ai, S. Y., Yin, H. S., Liu, X. G., & Qiu, Y. Y. (2012). Amperometric biosensor based on tyrosinase immobilized in hydrotalcite-like compounds film for the determination of polyphenols. Journal of Solid State Electrochemistry, 16, 449–456. DOI: 10.1007/s10008-011-1352-5.

    Article  CAS  Google Scholar 

  • Kochana, J., Kozak, J., Skrobisz, A., & Woźniakiewicz, M. (2012). Tyrosinase biosensor for benzoic acid inhibition-based determination with the use of a flow-batch monosegmented sequential injection system. Talanta, 96, 147–152. DOI: 10.1016/j.talanta.2011.12.009.

    Article  CAS  Google Scholar 

  • Krzyczmonik, P., Socha, E., & Skrzypek, S. (2015). Immobilization of glucose oxidase on modified electrodes with composite layers based on poly(3,4-ethylenedioxythiophene). Bioelectrochemistry, 101, 8–13. DOI: 10.1016/j.bioelechem.2014.06.009.

    Article  CAS  Google Scholar 

  • Li, X. R., Ren, T. K., Wang, A., & Ji, X. P. (2013). Gold nanoparticles-enhances amperometric tyrosinase biosensor based on three-dimensional sol-gel film-modified gold electrodes. Analytical Sciences, 29, 473–477. DOI: 10.2116/analsci.29.473.

    Article  CAS  Google Scholar 

  • Mai, A. T., Duc, T. P., Thi, X. C., Nguyen, M. H., & Nguyen, H. H. (2014). Highly sensitive DNA sensor based on polypyrrole nanowire. Applied Surface Science, 309, 285–289. DOI: 10.1016/j.apsusc.2014.05.032.

    Article  CAS  Google Scholar 

  • Medina-Plaza, C., de Saja, J. A., & Rodríguez-Méndez, M. L. (2014). Bioelectronic tongue based on lipidic nanostructured layers containing phenol oxidases and lutetium bisphthalocyanine for the analysis of grapes. Biosensors and Bioelectronics, 57, 276–283. DOI: 10.1016/j.bios.2014.02.023.

    Article  CAS  Google Scholar 

  • Mosnáčková, K., Chehimi, M., Fedorko, P., & Omastová, M. (2013). Polyamide grafted with polypyrrole: Formation, properties and stability. Chemical Papers, 67, 979–994. DOI: 10.2478/s11696-012-0305-5.

    Google Scholar 

  • Nowicka, A. M., Fau, M., Rapecki, T., & Donten, M. (2014). Polypyrrole-Au nanoparticles composite as suitable platform for DNA biosensor with electrochemical impedance spectroscopy detection. Electrochimica Acta, 140, 65–71. DOI: 10.1016/j.electacta.2014.03.187.

    Article  CAS  Google Scholar 

  • Park, E. S., Jang, D. H., Lee, Y. I., Jung, C. W., Lim, D. W., Kim, B. S., Jeong, Y. K., Myung, N. V., & Choa, Y. H. (2014). Fabrication and sensing property for conducting polymer nanowire-based biosensor for detection of immunoglobulin G. Research on Chemical Intermediates, 40, 2565–2570. DOI: 10.1007/s11164-014-1669-7.

    Article  CAS  Google Scholar 

  • Srinives, S., Sarkar, T., & Mulchandani, A. (2014). Primary amine-functionalized polyaniline nanothin film sensor for detecting formaldehyde. Sensors and Actuators B, 194, 255–259. DOI: 10.1016/j.snb.2013.12.079.

    Article  CAS  Google Scholar 

  • Sulka, G. D., Hnida, K., & Brzózka, A. (2013). pH sensors based on polypyrrole nanowire arrays. Electrochimica Acta, 104, 536–541. DOI: 10.1016/j.electacta.2012.12.064.

    Article  CAS  Google Scholar 

  • Tran, T. L., Chu, T. X., Huynh, D. C., Luu, T. H. T., & Mai, A. T. (2014). Effective immobilization of DNA for development of polypyrrole nanowires based biosensor. Applied Surface Science, 314, 260–265. DOI: 10.1016/j.apsusc.2014.06.068.

    Article  CAS  Google Scholar 

  • Vicentini, F. C., Janegitz, B. C., Brett, C. M. A., & Fatibello-Filho, O. (2013). Tyrosinase biosensor based on a glassy carbon electrode modified with multi-walled carbon nanotubes and 1-butyl-3-methylimidazolium chloride within a dihexadecylphosphate film. Sensors and Actuators B, 188, 1101–1108. DOI: 10.1016/j.snb.2013.07.109.

    Article  CAS  Google Scholar 

  • Xu, G. Q., Adeloju, S. B., Wu, Y. C., & Zhang, X. Y. (2012). Modification of polypyrrole nanowires array with platinum nanoparticles and glucose oxidase for fabrication of a novel glucose biosensor. Analytica Chimica Acta, 755, 100–107. DOI: 10.1016/j.aca.2012.09.037.

    Article  CAS  Google Scholar 

  • Zhang, L., Meng, F. L., Chen, Y., Liu, J. Y., Sun, Y. F., Luo, T., Li, M. Q., & Liu, J. H. (2009). A novel ammonia sensor based on high density, small diameter polypyrrole nanowire arrays. Sensors and Actuators B, 142, 204–209. DOI: 10.1016/j.snb.2009.07.042.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jolanta Kochana.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kochana, J., Hnida, K., Sulka, G. et al. Application of polypyrrole nanowires for the development of a tyrosinase biosensor. Chem. Pap. 69, 1130–1135 (2015). https://doi.org/10.1515/chempap-2015-0114

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1515/chempap-2015-0114

Keywords

Navigation