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Structural predication and antigenic analysis of Toxoplasma gondii ROP20

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Abstract

Toxoplasma gondii infects almost all the warm-blooded animals. ROP20 protein is expressed in the rhoptry of Toxoplasma gondii. In this study, the secondary structure of ROP20 was analyzed using SMART software. We constructed and analyzed the 3D model of ROP20 protein using SWISS-MODEL online procedure and Visual Molecular Dynamics (VMD) software. The structure analysis fully indicated that ROP20 protein is an important member of the ROP family. Furthermore, We used DNASTAR software and Epitope Database online service to analyze liner-B cell epitopes and T-cell epitopes of ROP20 protein. All the analysis results of ROP20 protein can provide positive information on treatment and vaccine for toxoplasmosis. Moreover, ROP20 gene was obtained from PCR, and a recombinant eukaryotic expression vector (pEGFP-C1-ROP20) was constructed in the following study. After restriction enzyme digestion, the constructed plasmid was transfected into HEK 293-T cells. The RT-PCR result indicated that the recombinant plasmid could transcribe successfully in HEK 293-T cell. The results of western blotting indicated the expressed proteins can be recognized by anti-STAg mouse sera.

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Authors and Affiliations

  1. Department of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People’s Republic of China

    Jian Zhou, Wanchun Wang & Xiadong Du

  2. Department of Sports Medicine Research Center, Central South University, Changsha, Hunan, 410008, People’s Republic of China

    Jian Zhou

  3. Department of Medicine, Quzhou College of Technology, Quzhou, Zhejiang, 324000, People’s Republic of China

    Pengxia Song

  4. Department of Electroneurophysiology, Jinan Children’s Hospital, Jinan, Shandong, 250022, People’s Republic of China

    Lin Wang

  5. Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong, 250012, People’s Republic of China

    Yali Han, Jingjing Guo, Zhen Hao & Shenyi He

  6. Department of Lymphoma and Hematology, Hunan Cancer Hospital, Changsha, Hunan, 410000, People’s Republic of China

    Xi Zhu

  7. University of South China, Hengyang, Hunan, 421001, People’s Republic of China

    Xi Zhu

  8. Department of Cardiology, The Fourth Hospital of Changsha, Changsha, Hunan, 410006, People’s Republic of China

    Qiong Zhou

  9. Department of Pathology, Taishan Medical College, Taian, Shandong, 271000, People’s Republic of China

    Gang Lu

  10. Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People’s Republic of China

    Yingquan Luo

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  1. Jian Zhou
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  2. Wanchun Wang
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  4. Lin Wang
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  8. Xi Zhu
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  9. Qiong Zhou
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  10. Xiadong Du
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  11. Gang Lu
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  12. Shenyi He
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  13. Yingquan Luo
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Correspondence to Yingquan Luo.

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Zhou, J., Wang, W., Song, P. et al. Structural predication and antigenic analysis of Toxoplasma gondii ROP20. Acta Parasit. 63, 244–251 (2018). https://doi.org/10.1515/ap-2018-0028

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  • DOI: https://doi.org/10.1515/ap-2018-0028

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