Abstract
Plasmodium vivax is the most common cause of malaria worldwide as well as southeastern Turkey. After the implementation of a successful national elimination program that the local malaria cases were not reported in 2011, malaria returned to county of Savur located in southeastern Turkey in summer of 2012. The present study aimed to determine the prevalent P. vivax genotypes isolated from southeastern Turkey. Genetic polymorphism in P. vivax CSP gene was analyzed by PCR-RFLP to assess the ratio of VK210 and VK247 types. Blood samples were obtained from 15 patients who lived in southeastern between 2005–2006. According to the results, VK210 type was detected in 10 samples (66.6%), VK247 type was observed in three samples (20%). Remaining two samples showed mixed infection (13.3%). The results of the present study first time showed the ratio of P. vivax genotypes in southeastern Turkey before the elimination in 2011. The results of the present study will be enable researchers to compare the new isolates with the previously detected ones and design new treatment and/elimination strategies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arnot D.E., Barnwell J.W., Tam J.P., Nussenzweig V., Nussenzweig R.S., Enea V. 1985. Circumsporozoite protein of Plasmodium vivax: gene cloning and characterization of the immunodominant epitope. Science, 230, 815–818
de Souza-Neiras W.C., de Melo L.M., Machado R.L. 2007. The genetic diversity of Plasmodium vivax—a review. Memórias do Instituto Oswaldo Cruz, 102, 245–254
Dias S., Wickramarachchi T., Sahabandu I., Escalante A.A., Udagama P.V. 2013. Population genetic structure of the Plasmodium vivax circumsporozoite protein (Pvcsp) in Sri Lanka. Gene, 518, 381–387
Hernández-Martínez M.Á., Escalante A.A., Arévalo-Herrera M., Herrera S. 2011. Antigenic diversity of the Plasmodium vivax circumsporozoite protein in parasite isolates of Western Colombia. The American Journal of Tropical Medicine and Hygiene, 84, 51–57.
Imwong M., Pukrittayakamee S., Grüner A.C., Rénia L., Letourneur F., Looareesuwan S., White N.J., Snounou G. 2005. Practical PCR genotyping protocols for Plasmodium vivax using Pvcs and Pvmsp1. Malaria Journal, 4, 20
Inspection and evaluation report of malaria outbreak occurred in Savur, Mardin, 2012. Turkish Medical Association publications.
Kain K.C., Brown A.E., Webster H.K., Wirtz R.A., Keystone J.S., Rodriguez M.H., Kinahan J., Rowland M., Lanar D.E. 1992. Circumsporozoite genotyping of global isolates of Plasmodium vivax from dried blood specimens. Journal of Clinical Microbiology, 30, 1863–1866
Leclerc M.C., Menegon M., Cligny A., Noyer J.L., Mammadov S., Aliyev N., Gasimov E., Majori G., Severini C. 2004. Genetic diversity of Plasmodium vivax isolates from Azerbaijan. Malaria Journal, 3, 40
Lopez A.C., Ortiz A., Coello J., Sosa-Ochoa W., Torres R.E., Banegas E.I., Jovel I., Fontecha G.A. 2012. Genetic diversity of Plasmodium vivax and Plasmodium falciparum in Honduras. Malaria Journal, 11, 391
Machado R.L., Póvoa M.M. 2000. Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment. Transactions of the Royal Society of Tropical Medicine and Hygiene, 94, 377–381
Malaria Surveillance Directorate statistics about Malaria, Ministry of Health, Republic of Turkey, 2014. http://www.saglik.gov.tr/TR/belge/1-3416/sitma-savas-daire-baskanliginin-sitma-ileilgili-istati-.html. Accessed November 18, 2014
Rosenberg R., Wirtz R.A., Lanar D.E., Sattabongkot J., Hall T., Waters A.P., Prasittisuk C. 1989. Circumsporozoite protein heterogeneity in the human malaria parasite Plasmodium vivax. Science, 245, 973–976
World Health Organization, 2012. Plasmodium vivax Control & Elimination: Development of Global Strategy and Investment Case, Malaria Policy Advisory Committee Meeting, http://www.who.int/malaria/mpac/sep2012/pvivax_development_global_strategy_and_investment_sep2012.pdf. Accessed November 18, 2014
World Health Organization, 2014. World Malaria Report 2012 http://www.who.int/malaria/publications/world_malaria_report_2012/en/index.html. Accessed November 18, 2014
Zakeri S., Abouie Mehrizi A., Djadid N.D., Snounou G. 2006. Circumsporozoite protein gene diversity among temperate and tropical Plasmodium vivax isolates from Iran. Tropical Medicine & International Health, 11, 729–737
Zakeri S., Raeisi A., Afsharpad M., Kakar Q., Ghasemi F., Atta H., Zamani G., Memon M.S., Salehi M., Djadid N.D. 2010b. Molecular characterization of Plasmodium vivax clinical isolates in Pakistan and Iran using pvmsp-1, pvmsp-3alpha and pvcsp genes as molecular markers. Parasitology International, 59, 15–21
Zakeri S., Safi N., Afsharpad M., Butt W., Ghasemi F., Mehrizi A.A., Atta H., Zamani G., Djadid N.D. 2010a. Genetic structure of Plasmodium vivax isolates from two malaria endemic areas in Afghanistan. Acta Tropica, 113, 12–19
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Döşkaya, A.D., Döşkaya, M., Caner, A. et al. Preliminary analysis of Plasmodium vivax genotypes isolated in southeastern Turkey. Acta Parasit. 60, 244–247 (2015). https://doi.org/10.1515/ap-2015-0034
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1515/ap-2015-0034