, Volume 72, Issue 2, pp 153–160 | Cite as

Development of an efficient Agrobacterium mediated transformation system for chickpea (Cicer arietinum)

  • Jaya Srivastava
  • Subhojit DattaEmail author
  • Sudhakar P. Mishra
Section Botany


Morphologically normal and fertile transgenic chickpea plants have been regenerated through a standardized transformation protocols. This protocol is based on the infection of apical meristem explants (AME) with Agrobacterium strain EHA105. The stain, carrying pCAMBIA2301 vector contained β-glucuronidase (uidA) gene and neomycin phosphotransferase (nptII) genes. Different explants of chickpea and Agrobacterium specific conditions were standardized with the help of transient β-glucuronidase (uidA) gene expression to further optimize the transformation protocol. Pre-conditiong of the explants, vacuum infiltration and presence of acetosyringone significantly enhanced the frequency of gus expression. Positive transformants with nptII and gus genes were confirmed by PCR and histochemical gus analysis. An overall successful chickpea transformation frequency of 1.2 was achieved. This high efficiency and easy to use method may provide opportunities for the development of transgenic lines with different useful genes in chickpea in near future.

Key words

Cicer arietinum chickpea Agrobacterium genetic transformation transformation 


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Copyright information

© Slovak Academy of Sciences 2017

Authors and Affiliations

  • Jaya Srivastava
    • 1
    • 2
  • Subhojit Datta
    • 1
    Email author
  • Sudhakar P. Mishra
    • 2
  1. 1.Biotechnology UnitICAR-Indian Institute of Pulses ResearchKanpurIndia
  2. 2.Mahatma Gandhi Chitrakoot Gramodaya VishvavidyalayaChitrakootIndia

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