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Biologia

, Volume 70, Issue 12, pp 1575–1585 | Cite as

Biochemical, anatomical and molecular level changes in cucumber (Cucumis sativus) seedlings exposed to copper oxide nanoparticles

  • Prakash M. Gopalakrishnan NairEmail author
  • Ill Min Chung
Section Cellular and Molecular Biology

Abstract

The effect of copper oxide nanoparticles (CuONPs) at the biochemical, anatomical and molecular level was investigated in cucumber (Cucumis sativus L.) seedlings. The seedlings were grown in semi-solid half strength Murashige and Skoog medium supplemented with 0, 50, 100, 200, 400 and 500 mg/L of CuONPs for fifteen days under controlled growth chamber conditions. The results showed that exposure to all concentrations of CuONPs resulted in significant reduction in shoot and root growth and biomass. A concentration-dependant increase in reactive oxygen species (ROS) generation, malondialdehyde production, lignin content and decline in mitochondrial membrane potential were observed. Cross-sections of stem showed anatomical changes, viz. an increase in xylogenesis in CuONPs exposed plants. Significant modulation in the expression of genes related to oxidative stress and lignin biosynthesis, i.e. catalase, ascorbate peroxidase, phenylalanine ammonia lyase, cinnamate 4-hydroxylase, anionic and cationic peroxidases were observed in shoots and roots as a result of CuONPs exposure. Taken together, exposure to CuONPs has resulted in excess ROS generation, lignification and growth suppression in Cucumis sativus seedlings.

Key words

Cucumis sativus; copper oxide nanoparticles oxidative stress lipid peroxidation lignification 

Abbreviations

APX

ascorbate peroxidase

CAT

catalase

CuONPs

copper oxide nanoparticles

C4H

cinnamate 4-hydroxylase

DAB

3’3’-diaminobenzidine

DHE

dihydroethidium

DW

dry weight

ENPs

engineered nanoparticles

FW

fresh weight

ͤΨm

mitochondrial membrane potential

HPF

3′-(p-hydroxyphenyl) fluorescein

MS

Murashige and Skoog

NBT

nitroblue tetrazolium

PAL

phenylalanine ammonia lyase

POD

peroxidase

ROS

reactive oxygen species

TMRM

tetramethylrhodamine methyl ester

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Copyright information

© Slovak Academy of Sciences 2015

Authors and Affiliations

  • Prakash M. Gopalakrishnan Nair
    • 1
    Email author
  • Ill Min Chung
    • 1
  1. 1.Department of Applied Biosciences, College of Life and Environmental SciencesKonkuk UniversitySeoulSouth Korea

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