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Biologia

, Volume 70, Issue 12, pp 1606–1613 | Cite as

Formation of radial symmetric needle-like rosette leaves in Arabidopsis

  • Jiang-Ping Song
  • Da-Hui Liu
  • Yi-Bo WangEmail author
  • Ya-Na ShiEmail author
Section Botany

Abstract

In the bodies of seed plants, lateral organs of the shoot, for example cotyledons, leaves, and floral organs, are determinate and exhibit localized planer growth resulting in breaking of radial symmetry and asymmetric development. Localized planer growth in the leaf generates the leaf blade, the principle site of photosynthesis in most plants. Development of flat and expanded lamina is an important physiological process. In these processes, a few kind of meristems cooperate with regulating leaf development. In this work, with transfer sense ASL11, sense ASL38 and antisense ASL15 under 35S promoter to to Arabidopsis (Col-0), we found a series of radial symmetric needle-like rosette leaves. By analyzing the anatomical and the epidermal cell features, our data suggests that in these malformed leaf blades, a few meristem tissues all can be suppressed or accelerated, and normal leaf blade morphology disappears. Pro ASL11:GUS expression further suggests that this process may be triggered by misexpression of a few transcription factors (Tfs).

Key words

Arabidopsis ASYMMERTIC LEAVES2-LIKE15 (ASL15/LBD17) gene ASL11/LBD15 gene ASL38/ LBD41 gene meristem tissues radial symmetric needle-like rosette leaves 

Abbreviations

RT-PCR

reverse transcription PCR

TFs

transcription factors

Kan

kanamycin

SAM

shoot apical meristems

Nptll

neomycin phosphotransferase II

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Copyright information

© Slovak Academy of Sciences 2015

Authors and Affiliations

  1. 1.Yuanpei CollegeShaoxing UniversityShamingP.R. China
  2. 2.Institute of Medicinal PlantsYunnan Academy of Agricultural SciencesKunmingP.R. China
  3. 3.Shool of Bioengineering and BiotechnologyTianshui Normal UniversityP.R. China

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