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Acta Parasitologica

, Volume 63, Issue 2, pp 368–374 | Cite as

Evaluation of the protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing BmAMA1 as vaccines against Babesia microti infection in hamster

  • Guanbo Wang
  • Longzheng Yu
  • Artemis Efstratiou
  • Paul Franck Adjou Moumouni
  • Mingming Liu
  • Huanping Guo
  • Yang Gao
  • Shinuo Cao
  • Mo Zhou
  • Jixu Li
  • Aaron Edmond Ringo
  • Xuenan XuanEmail author
Article

Abstract

In the present study, we have investigated the protective effect of a heterologous prime-boost strategy with priming plasmid DNA followed by recombinant adenovirus, both expressing BmAMA1, against Babesia microti infection. Four groups consisting of 3 hamsters per group were immunized with pBmAMA1/Ad5BmAMA1, pNull/Ad5BmAMA1, pBmAMA1/Ad5Null and pNull/Ad5Null, followed by challenge infection with B. microti. Our results showed that hamsters immunized with plasmid and adenovirus expressing BmAMA1 developed a robust IgG and IgG2a antibody response against BmAMA1, suggesting the DNA vaccine or viral vector vaccine tend to induce a Th1-biased response. Compared to the control hamsters, the hamsters vaccinated either with the prime-boost strategy or one of the two “vaccines” exhibited no significant protection against B. microti challenge. Although a slight difference in terms of parasitemia and hematocrit values at days 14–16 post challenge infection was observed, no other statistical difference was detected. Our results indicate that the prime-boost vaccination strategy of injection of plasmid and adenovirus expressing BmAMA1 is not efficient in protecting against B. microti infection.

Keywords

Babesia microti DNA vaccine Adenovirus Heterologous prime-boost BmAMA1 

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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2018

Authors and Affiliations

  • Guanbo Wang
    • 1
  • Longzheng Yu
    • 2
  • Artemis Efstratiou
    • 1
  • Paul Franck Adjou Moumouni
    • 1
  • Mingming Liu
    • 1
  • Huanping Guo
    • 1
  • Yang Gao
    • 1
  • Shinuo Cao
    • 3
  • Mo Zhou
    • 3
  • Jixu Li
    • 1
  • Aaron Edmond Ringo
    • 1
  • Xuenan Xuan
    • 1
    Email author
  1. 1.National Research Center for Protozoan DiseasesObihiro University of Agriculture and Veterinary MedicineInada-cho, ObihiroJapan
  2. 2.Department of Veterinary MedicineYanbian UniversityYanjiPeople’s Republic of China
  3. 3.State Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research Institute, Chinese Academy of Agricultural SciencesPeople’s Republic of China

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