Acta Parasitologica

, Volume 63, Issue 2, pp 333–345 | Cite as

First molecular characterization and morphological aspects of Sarcocystis fusiformis infecting water buffalo Bubalus bubalis in Egypt

  • Kareem Morsy
  • Fathy Abdel-Ghaffar
  • Saad Bin Dajem
  • Rewaida Abdel-GaberEmail author
  • Fatma El Gazar


Fresh muscle samples from water buffalo (Bubalus bubalis) aged 2–15, from Giza Province, Egypt; were examined for Sarcocystis infection. Macroscopic ovoid sarcocysts embedded in the muscle tissues of the examined buffaloes were detected; they measured 152–230 (210 ± 7) μm in length and 37–119 (95 ± 3) μm in width. The esophagus was the most infected organ followed by the diaphragm, and tongue, while the heart muscles were the least infected. The cyst cavity was compartmentalized by septa derived from the ground substance located under the primary cyst wall. Using transmission electron microscopy, the primary cyst wall bordered sarcocysts were determined to be 0.08–0.22 μm in thickness, raised from the parasitophorous vacuolar membrane, and surrounded by a secondary cyst wall of host origin. The primary cyst wall had irregular wall folds with numerous cauliflower-like projections of variable sizes and shapes accompanied by knob-like electron-dense elevations. 18S rRNA gene expression studies confirmed that the present parasite isolates belonged to the genus Sarcocystis. The sequence data showed significant identities (>90%) with archived gene sequences from many Eimeriidae organisms, and a dendogram showing the phylogenetic relationship was constructed. The most closely related species was Sarcocystis fusiformis KR186117, with an identity percentage of 98%. The recovered sequences were deposited in the GenBank under the accession number MG572125. The present study, to our knowledge, is the first collective ultrastructural and molecular study that confirmed the taxonomy of sarcocysts isolated from water buffaloes in Egypt as Sarcocystis fusiformis.


Sarcocystis fusiformis Bubalus bubalis light and transmission electron microscopy molecular study 


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  1. Abdel-Ghaffar, F., Al-Johany A.M. 2002. A light and electron microscope study of Sarcocystis mitrani (sp. nov.) infecting the skink Scincus mitranus in the central region of Saudi Arabia. Parasitology Research, 88, 102–106PubMedCrossRefGoogle Scholar
  2. Abdel-Ghaffar, F., Bashtar, A.R., Ashour, M.B., Sakran T.H. 1990. Life cycle of Sarcocystis gongyli Trinci 1911 in the skink Chalcides ocellatus and the snake Spalerosophis diadema. Parasitology Research, 76, 444–450CrossRefGoogle Scholar
  3. Abdel-Ghaffar, F., Heydorn, A.O., Mehlhorn H. 1989. The fine structure of cysts of Sarcocystis moulei from goats. Parasitology Research, 75, 416–418CrossRefGoogle Scholar
  4. Abdel-Ghaffar, F., Hilali, M., Scholtyseek E. 1978. Ultrastructure study of Sarcocystis fusiformis (Railliet 1897) infecting the Indian water buffalo (Bubalus bubalis) of Egypt. Tropical Medicine and Parasitology, 29, 289–294Google Scholar
  5. Abdel-Ghaffar, F., Mehlhorn, H., Bashtar, A.R., Al-Rasheid, K., Sakran T.H., Al-Fayoumi H. 2009. Life cycle of Sarcocystis camelicanis infecting the camel (Camelus dromedarius) and the dog (Canis familiaris), light and electron microscopic study. Parasitology Research, 106, 189–195PubMedCrossRefGoogle Scholar
  6. Abu-Elwafa, S., Al-Araby, M., Abbas I. 2015. Sarcocystis fusiformis infecting water buffaloes (Bubalus bubalis) in Dakahlia province, Egypt. International Journal of Advanced Research, 3, n116–120Google Scholar
  7. Ahmadi, M.M., Hajimohammadi, B., Eslami, G., Oryan, A., Yasini Ardakani S.A., Zohourtabar, A., Zare S. 2015. First identification of Sarcocystis hominis in Iranian traditional hamburger. Journal of Parasitic Diseases, 39, 770–772PubMedCrossRefGoogle Scholar
  8. Al-Hoot, A.S., Al-Qureishy, S.A., Al-Rashid, K., Bashtar A.R. 2005. Microscopic study on Sarcocystis moulei from sheep and goats in Saudi Arabia. Journal of the Egyptian Society of Parasitology, 35, 295–312PubMedGoogle Scholar
  9. Al-Quraishi, S.A., Bashtar, A.R., Al-Rasheid K.A.S., Abdel-Ghaffar F. 2005. The prevalence and ultrastructure of Sarcocystis species infecting camels (Camelus dromedaries) Slaughtered in Riyadh city, Saudi Arabia. Saudi Journal of Biological Sciences, 11, 135–142Google Scholar
  10. Barutzki, D., Schaper R. 2011. Results of parasitological examinations of faecal samples from cats and dogs in Germany between 2003 and 2010. Parasitology Research, 109, 545–560CrossRefGoogle Scholar
  11. Bashtar, A.R., Abdel-Ghaffar, F., El-Sayed M.A. 1988. Electron microscopic study on Sarcocystis capracanis infecting goats at Aswan area. Egyptian Journal of Histology, 11, 211–219Google Scholar
  12. Bledsoe B. 1980. Sarcocystis idahoensis sp. n. in deer mice Peromyscus maniculatus (wagner) and gopher snakes Pituophis melanoleucus (Daudin). The Journal of Protozoology, 27, 93–102PubMedCrossRefGoogle Scholar
  13. Calero-Bernal, R., Cerqueira-Cézar, C.K., Verma, S.K., Mowery, J., Carmena, D., Beckmen, K., Dubey J.P. 2016. Sarcocystis arctica (Apicomplexa: Sarcocystidae): ultrastructural description and its new host record, the Alaskan wolf (Canis lupus). Parasitology Research, 115, 2893–2897PubMedCrossRefGoogle Scholar
  14. Černá Ž. 1984. The role of birds as definitive hosts and intermediate hosts of heteroxenous coccidians. The Journal of Protozoology, 31, 579–581PubMedCrossRefGoogle Scholar
  15. Cerqueira-Cezar, C.K., Thompson, P.C., Verma, S.K., Mowery, J., Calero-Bernal, R., Antunes Murata, F.H., et al. 2017. Morphological and molecular characterization of Sarcocystis arctica like sarcocysts from the Arctic fox (Vulpes lagopus) from Alaska, USA. Parasitology Research, 116, 1871–1878PubMedCrossRefGoogle Scholar
  16. Chen, X., Wen, T., Hu, J., Liu, T., Esch, G.W., Liang, Y., et al. 2017. Sarcocystis dehongensis n. sp. (Apicomplexa: Sarcocystidae) from water buffalo (Bubalus bubalis) in China. Parasitology Research, 116, 2145–2150PubMedCrossRefGoogle Scholar
  17. Collier, L., Balows, A., Sussman M. 1998. Sarcocystis Isospora and Cyclospora. In: Gransden W.R, editor. Topley and Welson’s: Microbiology and Microbial Infections. 9th ed. Vol. 5. New York: Oxford University Press Inc, pp. 319–326Google Scholar
  18. Dahlgren, S., Gjerde B. 2007. Genetic characterization of six Sarcocystis species from reindeer (Rangifer tarand us tarand us) in Norway based on the small subunit rRNA gene. Veterinary Parasitology, 146, 204–213PubMedCrossRefGoogle Scholar
  19. Daptardar, M., Singh, B.B., Aulakh, R.S., Gill J.P 2016. Prevalence and first molecular identification of Sarcocystis species in cattle and water buffaloes in India. Acta Parasitologica, 61, 523–528PubMedCrossRefGoogle Scholar
  20. Dar, K.H., Bhat, M.M., Shafi M. 2017. Prevalence and organwise distribution of sarcocystiosis in buffaloes of Mohali, Punjab. Journal of Parasitic Diseases, 41, 318–321PubMedCrossRefGoogle Scholar
  21. Dubey, J.P., Lane, E.P., Wilpe, E., Suleman, E., Reininghaus, B., Verma S.K., et al. 2014. Sarcocystis cafferi n. sp. (Protozoa: Apicomplexa) from the African Buffalo (Syncerus caffer). Journal of Parasitology, 100, 817–827PubMedCrossRefGoogle Scholar
  22. Dubey, J.P., Moré G., van Wilpe, E., Calero-Bernal, R., Verma, S.K., Schares G. 2016. Sarcocystis rommeli, n. sp. (Apicomplexa: Sarcocystidae) from Cattle (Bostaurus) and its Differentiation from Sarcocystis hominis. Journal of Eukaryotic Microbiology, 63, 62–68PubMedCrossRefGoogle Scholar
  23. Dubey, J.P., Rosenthal, B.M., Morales, J.A., Alfaro A. 2006. Morphologic and genetic characterization of Sarcocystis sp. from the African grey parrot, Psittacus erithacus, from Costa Rica. Acta Parasitologica, 51, 161–168Google Scholar
  24. Dubey, J.P., Speer, C.A., Fayer R. 1989. Sarcocystosis of animals and man, 1st ed., CRC Press, Boca RatonGoogle Scholar
  25. Dubey, J.P., Naji, N., Mowery, J., Verma, S., Calero-Bernal R. 2017. Identification of macroscopic sarcocysts of sarcocystis cameli from one- humped camel (camelus dromedarius) in Iraq. Journal of Parasitology, 103, 168–169PubMedCrossRefGoogle Scholar
  26. El-Dakhly, K.M., El-Nesr, K.A., El-Nahass, S., Hirata, A., Sakai H., Yanai T. 2011. Prevalence and distribution patterns of Sarcocystis spp. In buffaloes in Beni-Suef, Egypt. Tropical Animal Health and Production, 43, 1549–1554PubMedCrossRefGoogle Scholar
  27. El-Ganayni S.E. 2003. Ultrastructure of Sarcocystis fayeri infecting donkeys (Equusasinus) in El-Menia Governorate, Egypt. Journal of the Egyptian-German Society of Zoology, 42, 33–46Google Scholar
  28. Ellis, T.J., Luton, K., Baverstock, P.R., Whitworth, G., Tenter A.M., Johnson A.M. 1995. Phylogenetic relationships between Toxoplasma and Sarcocystis deduced from a comparison of 18S rDNA sequences. Parasitology, 110, 521–528PubMedCrossRefGoogle Scholar
  29. El-Morsey, A., El-Seify, M., Desouky, A.R., Abdel-Aziz, M.M., El-Dakhly, K.M., Kasem, S., et al. 2015. Morphologic and molecular characteristics of Sarcocystis atraii n. sp. (Apicomplexa: Sarcocystidae) infecting the common coot (Fulica atra) from Egypt. Acta Parasitologica, 60, 691–9PubMedCrossRefGoogle Scholar
  30. El-Morsey, A., El-Seify, M., Desouky, A.R., Abdel-Aziz, M.M., Sakai H., Yanai T. 2013. Morphologic identification of a new Sarcocystis sp. in the common moorhen (Gallinula chloropus) (Aves: Gruiformes: Rallidae) from Brolos Lake, Egypt. Parasitology Research, 113, 391–397CrossRefGoogle Scholar
  31. Elsheikha, H.M., Kennedy, F.A., Murphy, A.J., Soliman, M., Mansfieled L.S. 2006. Sarcocystosis of Sarcocystis felis in cats. Journal of the Egyptian Society of Parasitology, 36, 1071–1079PubMedGoogle Scholar
  32. Entzeroth, R., Chobotar, B., Scholtyseck, E., Nemesri L. 1985. Light and electron microscope study of Sarcocystis sp. from the fallow deer (Cervivs lama). Parasitology Research, 71, 33–39PubMedGoogle Scholar
  33. Fayer R. 1972. Gametogeny of Sarcocystis in cell culture. Science, 175, 65–67PubMedCrossRefGoogle Scholar
  34. Fayer, R., Esposito, D.H., Dubeya J.P. 2015. Human infections with Sarcocystis species. Clinical Microbiology Reviews, 28, 295–311PubMedPubMedCentralCrossRefGoogle Scholar
  35. Ford G.E. 1987. Hosts of two canid genera, the red fox and the dog, as alternate vectors in the transmission of Sarcocystis tenella from sheep. Veterinary Parasitology, 26, 13–20PubMedCrossRefGoogle Scholar
  36. Fukuyo, M., Battsetseg, G., Byambaa B. 2002. Prevalence of Sarcocystis infection in horses in Mangolia. The Southeast Asian Journal of Tropical Medicine and Public Health, 33, 718–719PubMedGoogle Scholar
  37. Gillis, K.D., Mackay, R.J., Yowell, C.A., Levy, J.K., Greiner, E.C., Dame, J.B., et al. 2003. Naturally occurring sarcocystis infection in domestic cats (Felis catus). International Journal of Parasitology, 33, 877–883PubMedCrossRefGoogle Scholar
  38. Gjerde B. 2013. Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome c oxidase subunit I gene. International Journal of Parasitology, 43, 579–591PubMedCrossRefGoogle Scholar
  39. Gjerde B. 2016a. Molecular characterisation of Sarcocystis bovifelis, Sarcocystis bovini n. sp., Sarcocystis hirsuta and Sarcocystis cruzi from cattle (Bos taurus) and Sarcocystis sinensis from water buffaloes (Bubalus bubalis). Parasitology Research 115, 1473–1492PubMedCrossRefGoogle Scholar
  40. Gjerde B. 2016b. The resurrection of a species: Sarcocystis bovifelis Heydorn et al., 1975 is distinct from the current Sarcocystis hirsuta in cattle and morphologically indistinguishable from Sarcocystis sinensis in water buffaloes. Parasitology Research, 115, 1–21PubMedCrossRefGoogle Scholar
  41. Gjerde, B., Giacomelli, S., Bianchi, A., Bertoletti, I., Mondani, H., Gibelli L.R. 2017. Morphological and molecular characterization of four Sarcocystis spp., including Sarcocystis linearis n. sp., from roe deer (Capreolus capreolus) in Italy. Parasitology Research, 116, 1317–1338PubMedCrossRefGoogle Scholar
  42. Gjerde, B., Hilali M. 2016. Domestic cats (Feliscatus) are definitive hosts for Sarcocystis sinensis from water buffaloes (Bubalus bubalis). The Journal of Veterinary Medical Science, 78, 1217–1221PubMedPubMedCentralCrossRefGoogle Scholar
  43. Gjerde, B., Hilali, M., Abbas I.E. 2016. Molecular differentiation of Sarcocystis buffalonis and Sarcocystis levinei in water buffaloes (Bubalus bubalis) from Sarcocystis hirsuta and Sarcocystis cruzi in cattle (Bos taurus). Parasitology Research, 115, 2459–2471PubMedCrossRefGoogle Scholar
  44. Gjerde, B., Hilali, M., Mawgood S.A. 2015. Molecular characterization of three regions of the nuclear ribosomal DNA unit and the mitochondrial cox1 gene of Sarcocystis fusiformis from water buffaloes (Bubalus bubalis) in Egypt. Parasitology Research, 114, 3401–3413PubMedCrossRefGoogle Scholar
  45. Grikienienė J. 1994. Sarcocystis in cattle and swine: significance for man and man’s role in its spreading. Proceedings of the sci-entific conference Animal husbandary and ecology, Kaunas, Lithuania, pp. 76–80Google Scholar
  46. Hall T.A. 1999 BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98Google Scholar
  47. Hamidinejat, H., Razi Jalali, M.H., Gharibi, D., Molayan P.H. 2015. Detection of Sarcocystis spp. in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) in Iran by PCR-RFLP. Journal of Parasitic Diseases, 39, 658–62PubMedCrossRefGoogle Scholar
  48. Holmdahl, O.J., Mattsson M.J.G., Uggla, A., Johansson K.E. 1994. The phylogeny of Neos- poracaninum and Toxoplasma gondii based on ribosomal RNA sequences. FEMS Microbiology Letters, 119, 187–192PubMedCrossRefGoogle Scholar
  49. Holmdahl, O.J., Morrison, D.A., Ellis, J.T., Huong L.T. 1999. Evolution of ruminant Sarcocystis (Sporozoa) parasites based on small subunit rDNA sequences. Molecular Phylogenetics and Evolution, 11, 27–37PubMedCrossRefGoogle Scholar
  50. Hu, J.J., Huang, S., Wen, T., Esch, G.W., Liang, Y., Li H.L. 2017 Morphology, Molecular Characteristics, and Demonstration of a Definitive Host for Sarcocystis rommeli from Cattle (Bostaurus) in China. Journal of Parasitology, 103, 471–476PubMedCrossRefGoogle Scholar
  51. Hu, J.J., Wen, T., Chen, X.W., Liu, T.T., Esch, G.W., Huang S. 2016. Prevalence, Morphology, and Molecular Characterization of Sarcocystis heydorni Sarcocysts from Cattle (Bos Taurus) in China. Journal of Parasitology, 102, 545–548PubMedCrossRefGoogle Scholar
  52. Huong L.T.T., Dubey, J.P., Nikkilä T., Uggla A. 1997. Sarcocystis buffalonis n. sp. (Protozoa: Sarcocystidae) from the water buffalo (Bubalus bubalis) in Vietnam. Journal of Parasitology, 83, 471–474PubMedCrossRefGoogle Scholar
  53. Jäkel T. 1995. Cyclic transmission of Sarcocystis gerbilliechis sp. n. by the Arabian saw scaled viber, Echiscoloratus to rodents of the sub family Gerbillinae. Journal of Parasitology, 81, 626–631PubMedCrossRefGoogle Scholar
  54. JyothiSree, C., Venu, R., Samatha, V., Malakondaiah, P., Rayulu V.C. 2017. Prevalence and microscopic studies of Sarcocystis infection in naturally infected water buffaloes (Bubalusbubalis) of Andhra Pradesh. Journal of Parasitic Diseases, 41, 476–482PubMedCrossRefGoogle Scholar
  55. Khalifa, R.M., El-Nadi, N.A., Sayed, F.G., Omran E.K. 2008. Comparative morphological studies on three Sarcocystis species in Sohag, Journal of the Egyptian Society of Parasitology, 38, 599–608PubMedGoogle Scholar
  56. Latif, B., Kannan Kutty, M., Muslim, A., Hussaini, J., Omar, E., Heo C.C., et al. 2015. Light microscopy and molecular identification of Sarcocystis spp. in meat producing animals in Selangor, Malaysia. Tropical Biomedicine, 32, 444–452PubMedGoogle Scholar
  57. Li, Q.Q., Yang, Z.Q., Zuo, Y.X., Attwood, S.W., Chen, X.W., Zhang Y.P. 2002. A PCR-based RFLP analysis of Sarcocystiscruzi (Protozoa: Sarcocystidae) in Yunnan province, PR China, reveals the water buffalo (Bubalus bubalis) as a natural intermediate host. Journal of Parasitology, 88, 1259–1261PubMedCrossRefGoogle Scholar
  58. Lukesová D., Nevole, M., Haidová B. 1986. The extent of the incidence of sarcocystosis in cattle and pig farms. Veterinary Medicine, 31, 521–530Google Scholar
  59. Matuschka F.R. 1986. Sarcocystis clethrionomyelaphis n. sp. from snakes of the genus Elaphe and defferent voles of the family Arvicolidae. Journal of Parasitology, 72, 226–231CrossRefGoogle Scholar
  60. Matuschka, F.R., Bannert B. 1989. Recognition of cyclic transmission of Sarcocystis stehlinii n. sp. in the Gran Canarian giant lizard. Journal of Parasitology, 75, 383–387PubMedCrossRefGoogle Scholar
  61. Matuschka, F.R., Mehlhorn, H., Abd-Al-Aal Z. 1987. Replacement of Besnoitia Matuschka and Häfner 1984 by Sarcocystis hoarensis. Parasitology Research, 74, 94–96PubMedCrossRefGoogle Scholar
  62. Mehlhorn H. 2008. Encylopedic reference of parasitology 3rd ed. Springer, Verlag, BerlinGoogle Scholar
  63. Mehlhorn, H., Hartly, W.J., Heydorn A.O. 1976. A comparative ultrastructural study of the cyst wall of 13 Sarcocystis species. Protistologica, 12, 451–467Google Scholar
  64. Mehlhorn, H., Heydorn A.O. 1978. Sarcosporidia (Protozoa, Sporozoa): Life cycle and fine structure. Advances in Parasitology, 16, 73–92Google Scholar
  65. Mehlhorn, H., Scholtyseck E. 1973. Elektron en mikrokopischeUntersuchungen an Cystenstadien von Sarcocysti stenella aus der OesophagusMuskulatur des Schafes. Parasitology Research, 41, 291–310PubMedGoogle Scholar
  66. Metwally, A.M., Abd-Ellah, M.R., Al-Hosary, A.A., Omar M.A. 2014. Microscopical and serological studies on Sarcocystis infection with first report of Sarcocystis cruzi in buffaloes (Bubalus bubalis) in Assiut, Egypt. Journal of Parasitic Diseases, 38, 378–82PubMedCrossRefGoogle Scholar
  67. Modrý D., Koudela, B., Slapeta J.R. 2000. Sarcocystis stenodactylicolubris n. sp., a new sarcosporidian coccidium with a snakegecko heteroxenous life cycle. Parasite, 7, 201–207PubMedCrossRefGoogle Scholar
  68. Mollenhauer H.H. 1964. Plastic embedding mixtures for use in electron microscopy. Stain Technology, 39, 111–114PubMedGoogle Scholar
  69. Moré G., Abrahamovich, P., Jurado, S., Bacigalupe, D., Marin, J.C., Rambeaud, M., et al. 2011. Prevalence of Sarcocystis spp. in Argentinean cattle. Veterinary Parasitology, 177, 162–165PubMedCrossRefGoogle Scholar
  70. Moré G., Regensburger, C., Gos, L., Pardini, L., Verma, S., Ctibor, J., et al. 2016. Sarcocystis masoni, n. sp. (Apicomplexa: Sarcocystidae), and redescription of Sarcocystis aucheniae from llama (Lama glama), guanaco (Lama guanicoe) and alpaca (Vicugna pacos). Parasitology, 143, 617–626PubMedCrossRefGoogle Scholar
  71. Morsy, K., Saleh, A., Al-Ghamdi, A., Abdel-Ghaffar, F., Al-Rasheid, K., Bashtar, A.R., et al. 2011. Prevalence pattern and biology of Sarcocystis capracanis infection in the Egyptian goats: a light and ultrastructural study. Veterinary Parasitology, 181, 75–82PubMedCrossRefGoogle Scholar
  72. Olias, P.P., Olias, L., Krücken, J., Lierz, M., Gruber A.D. 2011. High prevalence of Sarcocystis calchasi sporocysts in European Accipiter hawks. Veterinary Parasitology, 175, 230–236PubMedCrossRefGoogle Scholar
  73. Olsen, G.J., Woese C.R. 1993. Ribosomal RNA: A key to phylogeny. Federation of American Societies for Experimental Biology Journal, 1, 113–123CrossRefGoogle Scholar
  74. Oryan, A., Moghaddar, N., Gaur S.N.S. 1996. The distribution pattern of Sarcocystis sp., their transmission and pathogenesis in sheep in Fars province of Iran. Veterinary Research Communications, 20, 243–253PubMedCrossRefGoogle Scholar
  75. Prakas, P., Kutkienė L., Butkauskas, D., Sruoga, A., Zalakevičius M. 2013. Molecular and morphological investigations of Sarcocystis corvusi sp. nov. from the jackdaw (Corvusmonedula). Parasitology Research, 112, 1163–1167PubMedCrossRefGoogle Scholar
  76. Rommel, M., Heydorn, A.O., Gruber V. 1972. Beitragezum Lebenszyklus der Sarkosporidien. I. Die Sporozyste von S. tenella in den Fazes der Katze. Berliner und Münchener tierärztliche Wochenschrift, 85, 101–105. (In German)PubMedGoogle Scholar
  77. Sakran, T., Al-Hroub, A., Ahmed A. 2013. Studies on Sarcocystis infecting domestic horse. American Journal of Research Communication, 1, 39–53Google Scholar
  78. Scholtyseck, E., Mehlhorn, H., Miiller B.E.G. 1974. Feinstruktur der Cyste und Cystenw and von Sarcocystis tenella, Besnoitia jellisoni, Frenkelia sp. und Toxoplasma gondii. The Journal of Protozoology, 21, 284–294CrossRefGoogle Scholar
  79. Scioscia, N.P., Olmos, L., Gorosábel, A., Bernad, L., Pedrana, J., Hecker Y.P., et al. 2017. Pampas fox (Lycalopex gymnocercus) new intermediate host of Sarcocystis svanai (Apicomplexa: Sarcocystidae). Parasitology International, 66, 214–218PubMedCrossRefGoogle Scholar
  80. Stina, S., Dahlgren, S.S., Gjerde B. 2008. Molecular identification and phylogeny of six Sarcocystis species in moose, and a morphological description of three new species. Parasitology Research, 103, 93–110CrossRefGoogle Scholar
  81. Stolte, M., Odening, K., Quand, T.S., Bengis, R.G., Bockhardt I. 1998. Sarcocystis dubeyella n. sp. and Sarcocystis phacochoeri n. sp. (Protozoa: Sarcocystidae) from the warthog (Phaco-choerus aethiopicus) in South Africa. Journal of Eukaryotic Microbiology, 45, 101–104PubMedCrossRefGoogle Scholar
  82. Tamura, K., Dudley, J., Nei, M., Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599PubMedCrossRefGoogle Scholar
  83. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins D.G. 1997. The Clustal-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882PubMedPubMedCentralCrossRefGoogle Scholar
  84. Verma, S.K., Lindsay, D.S., Mowery, J.D., Rosenthal, B.M., Dubey J.P. 2017. Sarcocystis pantherophisi n. sp., from Eastern Rat Snakes (Pantherophis alleghaniensis) as Definitive Hosts and Interferon Gamma Gene Knockout Mice as Experimental Intermediate Hosts. Journal of Parasitology, 103, 547–554PubMedCrossRefGoogle Scholar
  85. Yan, W., Qian, W., Li, X., Wang, T., Ding, K., Huang T. 2013. Morphological and molecular characterization of Sarcocystis miescheriana from pigs in the central region of China. Parasitology Research, 112, 975–980PubMedCrossRefGoogle Scholar
  86. Yang, Z.Q., Zuo, Y.X., Yao, Y.G., Chen, X.W., Yang, G.C., Zhang Y.P. 2001. Analysis of the 18S rRNA genes of Sarcocystis species suggests that the morphologically similar organisms from cattle and water buffalo should be considered the same species. Molecular and Biochemical Parasitology, 115, 283–288PubMedCrossRefGoogle Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2018

Authors and Affiliations

  • Kareem Morsy
    • 1
    • 2
  • Fathy Abdel-Ghaffar
    • 2
  • Saad Bin Dajem
    • 1
  • Rewaida Abdel-Gaber
    • 2
    • 3
    Email author
  • Fatma El Gazar
    • 2
  1. 1.Biology Department, College of ScienceKing Khalid UniversityAbhaSaudi Arabia
  2. 2.Zoology Department, Faculty of ScienceCairo UniversityCairoEgypt
  3. 3.Zoology Department, College of ScienceKing Saud UniversityRiyadhSaudi Arabia

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