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Acta Parasitologica

, Volume 63, Issue 2, pp 299–303 | Cite as

Genetic diversity of Taenia hydatigena in the northern part of the West Bank, Palestine as determined by mitochondrial DNA sequences

  • Kamel Adwan
  • Alaa Jayousi
  • Sameh Abuseir
  • Ibrahim Abbasi
  • Ghaleb AdwanEmail author
  • Naser Jarrar
Article

Abstract

Cysticercus tenuicollis is the metacestode of canine tapeworm Taenia hydatigena, which has been reported in domestic and wild ruminants and is causing veterinary and economic losses in the meat industry. This study was conducted to determine the sequence variation in the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene in 20 isolates of T. hydatigena metacestodes (cysticercus tenuicollis) collected from northern West Bank in Palestine. Nine haplotypes were detected, with one prevailing (55%). The total haplotype diversity (0.705) and the total nucleotide diversity (0.0045) displayed low genetic diversity among our isolates. Haplotype analysis showed a star-shaped network with a centrally positioned common haplotype. The Tajima’s D, and Fu and Li’s statistics in cysticercus tenuicollis population of this region showed a negative value, indicating deviations from neutrality and both suggested recent population expansion for the population. The findings of this study would greatly help to implement control and preventive measures for T. hydatigena larvae infection in Palestine.

Keywords

Taenia hydatigena genetic variation cysticercus tenuicollis cytochrome c oxidase subunit I Palestine 

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References

  1. Bandelt, H.J., Forster, P., Röhl A. 1999. Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16, 37–48CrossRefGoogle Scholar
  2. Boufana, B., Scala, A., Lahmar, S., Pointing, S., Craig, P.S., Dessì G., et al. 2015a. A preliminary investigation into the genetic variation and population structure of Taenia hydatigena from Sardinia, Italy. Veterinary parasitology. 214, 67–74. DOI: 10.1016/j.vetpar.2015.08.003CrossRefGoogle Scholar
  3. Boufana, B., Lett, W., Lahmar, S., Griffiths, A., Jenkins, D.J., Buishi, I., et al. 2015b. Canine echinococcosis: genetic diversity of Echinococcus granulosus s ensu stricto (ss) from definitive hosts. Journal of Helminthology, 89, 689–698. DOI: 10.1017/S0022149X15000395CrossRefGoogle Scholar
  4. Bowles, J., Blair, D., McManus D.P. 1992. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology, 54, 165–173CrossRefGoogle Scholar
  5. Dajani, Y.F., Khalaf F.H. 1981. Hydatidosis and tenuicollosis in sheep and goats of Jordan: a comparative study. Pathogens and global Health, 75, 175–179Google Scholar
  6. Hasslinger, M.A., Weber-Werringhen R. 1988. Fecal surveys in pastured sheep and the occurrence of cysticercus tenuicollis in slaughtered sheep. Angewandte Parasitologie, 29, 227–234PubMedGoogle Scholar
  7. Librado, P., Rozas J. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451–1452. DOI: 10.1093/bioinformatics/btp187CrossRefGoogle Scholar
  8. Mekuria, E., Shimelis, S., Bekele, J., Sheferaw D. 2013. Sheep and goats cysticercus tenuicollis prevalence and associated risk factors. African Journal of Agricultural Research, 8, 3121–3125. DOI: 10.5897/AJAR2012.7361Google Scholar
  9. Murrell, KD. (Ed.). 2005. Guidelines for the surveillance, prevention and control of taeniosis/cysticercosis. Paris, France: OIE/WHO/FAO. pp. 139Google Scholar
  10. Nakao, M., Sako, Y., Ito A. 2003. Isolation of polymorphic microsatellite loci from the tapeworm Echinococcus multilocularis. Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 3, 159–163CrossRefGoogle Scholar
  11. Nimbalkar, R.K., Shinde, S.S., Kamitikar, V.N., Muley S.P. 2011. Study on Taenia hydatigena in the slaughtered sheep (Ovis bharal) and goats (Capra hircus) in Maharashtra, India. Global Veterinaria, 6, 374–377Google Scholar
  12. Nourani, H., Pirali Kheirabadi, K.H., Rajabi, H., Banitalebi A. 2010. An unusual migration of Taenia hydatigena larvae in a lamb. Tropical Biomedicine, 27, 651–656PubMedGoogle Scholar
  13. Oryan, A., Goorgipour, S., Moazeni, M., Shirian S. 2012. Abattoir prevalence, organ distribution, public health and economic importance of major metacestodes in sheep, goats and cattle in Fars, southern Iran. Tropical Biomedicine, 29, 349–359PubMedGoogle Scholar
  14. Ramos-Onsins, S.E., Rozas J. 2002. Statistical properties of new neutrality tests against population growth. Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 19, 2092–2100Google Scholar
  15. Rostami, S., Salavati, R., Beech, R.N., Babaei, Z., Sharbatkhori, M., Baneshi M.R. et al. 2015. Molecular and morphological characterization of the tapeworm Taenia hydatigena (Pallas, 1766) in sheep from Iran. Journal of Helminthology, 89, 150–157CrossRefGoogle Scholar
  16. Samuel, W., Zewde, G.G. 2010. Prevalence, risk factors, and distribution of cysticercus tenuicollis in visceral organs of slaughtered sheep and goats in central Ethiopia. Tropical animal health and production. Tropical Animal Health and Production, 42, 1049–1051. DOI: 10.1007/s11250-010-9537-yCrossRefGoogle Scholar
  17. Scala, A., Pipia, A.P., Dore, F., Sanna, G., Tamponi, C., Marrosu, R., et al. 2015. Epidemiological updates and economic losses due to Taenia hydatigena in sheep from Sardinia, Italy. Parasitology Research, 114, 3137–3143. DOI: 10.1007/s00436-015-4532-xCrossRefGoogle Scholar
  18. Senlik B. 2008. Influence of host breed, sex and age on the prevalence and intensity of cysticercus tenuicollis in sheep. Journal of Animal and Veterinary Advances, 7, 548–551Google Scholar
  19. Soulsby E.J.L. 1982. Helminths, arthropods and protozoa of domesticated animals, 7th ed. Bailliere Tindall, London, pp. 809Google Scholar
  20. Sultan, K., Desoukey, A.Y., Elsiefy, M.A., Elbahy N.M. 2010. An abattoir study on the prevalence of some gastrointestinal helminths of sheep in Gharbia Governorate, Egypt. Global Veterinaria, 5, 84–87Google Scholar
  21. Yamasaki, H., Nakao, M., Sako, Y., Nakaya, K., Ito, A., 2005. Molecular identification of Taenia solium cysticercus genotype in the histopathological specimens. The Southeast Asian Journal of Tropical Medicine and Public Health, 36, 131–134PubMedGoogle Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2018

Authors and Affiliations

  • Kamel Adwan
    • 1
  • Alaa Jayousi
    • 1
  • Sameh Abuseir
    • 2
  • Ibrahim Abbasi
    • 3
  • Ghaleb Adwan
    • 1
    Email author
  • Naser Jarrar
    • 1
  1. 1.Department of Biology and BiotechnologyAn-Najah National UniversityNablusPalestine
  2. 2.Faculty of Veterinary MedicineAn-Najah N. UniversityNablusPalestine
  3. 3.Department of Biology and BiotechnologyAl-Quds UniversityJerusalemPalestine

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