Morphological, histological and molecular characterization of Myxobolus kingchowensis and Thelohanellus cf. sinensis infecting gibel carp Carassius auratus gibelio (Bloch, 1782)
A Myxobolus species and a Thelohanellus species infecting Carassius auratus gibelio (Bloch, 1782) were redescribed by their morphological, histological and molecular characterization. In the present study, the Myxobolus species infecting the muscle was identified as Myxobolus kingchowensis Chen et Ma, 1998 by the morphological and molecular data. Histologically, mature spores of M. kingchowensis were observed in the intercellular and connective tissue of muscle, though the plasmodia were not found. In addition, scattered spores also occurred in the intercellular of haematopoietic cells, intraepithelial of the renal tubules and interior of the melano-macrophage centres. Phylogenetic analysis showed that M. kingchowensis clustered in the clade of muscle-infecting Myxobolus species, further supporting muscle as the infection site of M. kingchowensis. The present Thelohanellus species infecting the gills was identified conspecific as Thelohanellus sinensis reported in Sun (2006) (mark it as T. sinensis-Sun) based on spore morphology, biological traits (host specificity and organ specificity), and molecular data. However, compared with the original description of T. sinensis Chen et Hsieh, 1960, the present Thelohanellus species and T. sinensis-Sun both infecting the gills of gibel carp are distinguishable from the original description in the host and infection site, which made the validity of T. sinensis-Sun dubious. Due to the absence of molecular data in the original description of T. sinensis, we suggest marking the present species and T. sinensis-Sun as T. cf. sinensis to avoid the confusion until T. sinensis is obtained from the type host and type infection site.
KeywordsMyxosporea Myxobolus kingchowensis Thelohanellus sinensis Carassius auratus gibelio China
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- Atkinson, S.D., Bartošová-Sojková P., Whipps, C.M., Bartholomew J.L. 2015. Approaches for characterising myxozoan species. (Eds B. Okamura, A. Gruhl, J. L. Bartholomew) Myxozoan Evolution, Ecology and Development, Cham, Springer. pp. 111–123. DOI: https://doi.org/10.1007/978-3-319-14753-6_6CrossRefGoogle Scholar
- Chen C.L. 1973. An illustrated guide to the fish diseases and causative pathogenic fauna and flora in the Hubei Province. Inst. Hydrobiol., Publishing House Science, Beijing, China. (In Chinese)Google Scholar
- Chen, C.L., Hsieh S.R. 1960. Studies on sporozoa from the freshwater fishes Ophiocephalus maculatus and O. argus of China. Actc Hydrobiologica Sinica, 2, 171–196Google Scholar
- Chen, Q.L., Ma C.L. 1998. Myxozoa: Myxosporea. Science Press, Beijing. (In Chinese)Google Scholar
- Hartigan, A., Wilkinson, M., Gower, D.J., Streicher, J.W., Holzer, A.S., Okamura B. 2016. Myxozoan infections of caecilians demonstrate broad host specificity and indicate a link with human activity. International Journal for Parasitology, 46, 375–381. DOI: https://doi.org/10.1016/j.ijpara.2016.02.001CrossRefGoogle Scholar
- Liu, Y., Zhai, Y.H., Gu Z.M. 2016b. Morphological and molecular characterization of Thelohanellus macrovacuolaris n. sp. Myxosporea: Bivalvulida infecting the palate in the mouth of common carp Cyprinus carpio L. in China. Parasitology International, 65, 303–307. DOI: https://doi.org/10.1016/j.parint.2016.02.013CrossRefGoogle Scholar
- Maciel, P.O., Affonso, E.G., Boijink C.D.L., Tavaresdias, M., Inoue L.A.K.A. 2011. Myxobolus sp. (Myxozoa) in the circulating blood of Colossoma macropomum (Osteichthyes, Characidae). Revista Brasileira De Parasitologia Veterinaria, 20, 82–84. DOI: 10.1590/S1984-29612011000100018CrossRefGoogle Scholar
- Molnár K. 1994. Comments on the host, organ and tissue specificity of fish myxosporeans and on the types of their intrapiscine development. Parasitology Hung, 27, 5–20Google Scholar
- Sun C.Y. 2006. Morphological and molecular comparison of myxosporeans. Master Thesis, Chong Qing Normal University, Chongqing, China. (In Chinese)Google Scholar
- Whipps, C.M., Adlard, R.D., Bryant, M.S., Lester, R.J., Findlay, V., Kent M.L. 2003. First report of three Kudoa species from eastern Australia: Kudoa thyrsites from mahi mahi Coryphaena hippurus, Kudoa amamiensis and Kudoa minithyrsites n. sp. from sweeper Pempheris ypsilychnus. Journal of Eukaryotic Microbiology, 50, 215–219. DOI: 10.1111/j.1550-7408.2003.tb00120.xCrossRefGoogle Scholar
- Yuan, S., Xi, B., Wang, J., Xie, J., Zhang J. 2015. Thelohanellus wangi n. sp. Myxozoa, Myxosporea, a new gill parasite of allogynogenetic gibel carp Carassius auratus gibelio Bloch in China, causing severe gill myxosporidiosis. Parasitology Research, 114, 37–45. DOI: https://doi.org/10.1007/s00436-014-4157-5CrossRefGoogle Scholar