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Acta Parasitologica

, Volume 63, Issue 1, pp 184–189 | Cite as

Molecular and serological investigation of infectious diseases in captive and free-range crab-eating fox (Cerdocyon thous — Linnaeus, 1776) from northeastern Brazil

  • Jonatas C. AlmeidaEmail author
  • Renata P. B. Melo
  • Pomy C. P. Kim
  • Neurisvan R. Guerra
  • Leucio C. Alves
  • Diego F. Costa
  • Clebert José Alves
  • Wagnner J. N. Porto
  • Rinaldo A. Mota
Article

Abstract

The aim of this study was to detect DNA and antibodies anti-Leishmania spp., Neospora caninum and Toxoplasma gondii in captive and free-range crab-eating fox (Cerdocyon thous) from northeastern Brazil. Twenty-five crab-eating foxes from different states of northeastern Brazil were sampled by this study. Blood samples were collected by cephalic or jugular vein punctures. The whole blood was submitted to PCR, and the sera samples to the serological analysis as follows: MAT for T. gondii, NAT for N. caninum, and ELISA for L. chagasi. The frequence of antibodies anti-T. gondii was 50% and 29.41% for free-range and captive wild canids, respectively. The frequence of antibodies anti-N. caninum observed by this study was 62.50% and 23.52% for free-range and captive wild canids, respectively. The frequence of antibodies anti-L. chagasi was 4.0% for captive wild canids. Co-infections cases were identified as follows: one captive wild canid seropositive for T. gondii and L. chagasi and two free-range animals seropositive for T. gondii and N. caninum. All PCR assays performed were negative for the pathogens analyzed. This study describes the presence of antibodies anti-T. gondii, N. caninum e L. chagasi in wild canids from northeastern Brazil and highlights the necessity of further studies on infectious diseases in free-range and captive wild canids.

Keywords

Leishmania Neospora Toxoplasma wild canids 

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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2018

Authors and Affiliations

  • Jonatas C. Almeida
    • 1
    Email author
  • Renata P. B. Melo
    • 1
  • Pomy C. P. Kim
    • 1
  • Neurisvan R. Guerra
    • 2
  • Leucio C. Alves
    • 2
  • Diego F. Costa
    • 3
  • Clebert José Alves
    • 3
  • Wagnner J. N. Porto
    • 4
  • Rinaldo A. Mota
    • 1
  1. 1.Department of Veterinary Medicine, Laboratory of Infectious-Contagious Diseases of Domestic AnimalsUniversidade Federal Rural de PernambucoRecifeBrazil
  2. 2.Department of Veterinary Medicine, Laboratory of Parasitology and EpidemiologyUniversidade Federal Rural de PernambucoRecifeBrazil
  3. 3.Department of Veterinary MedicineUniversidade Federal de Campina GrandePatosBrazil
  4. 4.Department of Veterinary Medicine, Laboratory of Parasitology and Parasitic DiseasesUniversidade Federal de Alagoas, Fazenda São LuísViçosaBrazil

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