Advertisement

Acta Parasitologica

, Volume 62, Issue 3, pp 652–665 | Cite as

Redescription of two species of Thaparocleidus (Monogenea: Dactylogyridae), with the description of T. armillatus sp. n. from Wallago attu and a phylogenetic analysis based on 18S rDNA sequences

  • Chandni VermaEmail author
  • Anshu Chaudhary
  • Hridaya Shanker Singh
Article

Abstract

The phylogenetic studies on monogeneans of the genus Thaparocleidus parasitizing W. attu in India was inferred from 18S rDNA gene data. Out of species of Thaparocleidus, one new, T. armillatus sp. n., is described herein which distinguished from its congener species in the shapes and sizes of sclerotized structures and rest of the two species, T. indicus and T. wallagonius are redescribed morphologically as well as validated and confirmed by molecular means also. Besides the above, T. gangus was also included in the molecular analysis as it is recently described on the basis of morphology only. The phylogenetic tree was also reconstructed in order to elucidate the taxonomic status of Thaparocleidus armillatus sp. n., T. indicus, T. wallagonius and T. gangus. This research reports for the first time, the molecular phylogenetic analysis of 18S rDNA gene for four species belonging to the genus Thaparocleidus from W. attu collected off the River Ganga in India. In phylogram, all four species of Thapaocleidus come in a single clade that confirmed their monophyletic status. A very low interspecific variability was observed in T. armillatus sp. n. and T. wallagonius suggesting that these species are highly similar in morphology. Correspondingly, T. indicus and T. gangus also showed low interspecific variability which too signifies their morphological similarities in their sclerotized structures. Our result suggested that all four species taken in the present investigation from India are monophyletic and it is also observed that morphological similarities in the sclerotized structures are well correlated with phylogeny.

Keywords

Monogenea Thaparocleidus spp. morphology molecular 18S rDNA India 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Akhmerov A.K. 1964. On the evolution of the median attachment apparatus of the monogenetic suborder Dactylogyrinea. Trudy Gel’minthologicheskoi Laboratorii, 14, 69–79 (In Russian)Google Scholar
  2. Agrawal N., Sharma R. 1988. On a new monogenea Neomurraytrema lucknowensis sp. nov. from Mystus vittatus Bloch). Indian Journal of Parasitology, 12, 61–63Google Scholar
  3. Agrawal N., Vishwakarma, P., Shukla R., Devak A. 2005. A note on surface topography of Thaparocleidus indicus (Kulkarni, 1969) Lim, 1966 on Wallago attu (Bloch and Schn.). National Journal of Life Science, 2, 131–132Google Scholar
  4. Caballero Y.C.E., Bravo-Hollis, M. 1961. Trematodes de peces de aguas Mexicanas del Pacifico. XVIII. Un nuevo género y una nueva especie de Monogenoidea Byjovskii, 1937. Anales del Instituto de Biología, 31, 197–205. (In Spanish)Google Scholar
  5. Chaudhary A., Singh H.S. 2012. Phylogenetic study of nine species of freshwater monogeneans using secondary structure and motif prediction from India. Bioinformation, 8, 862–869CrossRefGoogle Scholar
  6. Chaudhary A., Singh H.S. 2012. Description of two new species of the genus Thaparocleidus Jain, 1952 (Monogenea, Dactylogyridae) from freshwater fish in India: morphological and molecular phylogenetic evidence. Journal of Helminthology, 87, 160–173CrossRefGoogle Scholar
  7. Chaudhary A., Verma C., Varma M., Singh H.S. 2014. Identification of Thaparocleidus caecus (Monogenea: Dactylogyridae) using morphological and molecular tools: a parasite invasion in Indian freshwater. Bioinvasions Records, 3, 195–200CrossRefGoogle Scholar
  8. Dubey A., Gupta A.K., Agarwal S.M. 1992. Studies on monogenean parasites in freshwater fishes at Raipur. VIII. Validity of Parancylodiscoides Akhmerov, 1964 (Silurodiscoides Gusev, 1974) and two new species from Raipur. Indian Journal of Helminthology, 44, 9–16Google Scholar
  9. Froese R., Pauly D. 2002. Fish Base: World Wide Web electronic publication. http://www.fishbase.orgGoogle Scholar
  10. Gusev A.V. 1973. Monogeneans of freshwater fishes of India and an analysis of the world fauna of the group. Abstract of Doctor of Biological Science Thesis, Leningrad: Akademiya Nauk, pp. 31Google Scholar
  11. Gusev A.V. 1976. Freshwater Indian Monogenoidea. Principles of systematics, analysis of the world faunas and their evolution. Indian Journal of Helminthology, 25 and 26 (1973 and 1974), 1–241Google Scholar
  12. Hall T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98Google Scholar
  13. Jain S.L. 1952. Monogenea of Indian freshwater fishes. II. Thaparocleidus wallagonius n.g. and n. sp. (subfamily Tetraonchinae) from the gills of Wallagonia attu (Bloch) from Lucknow. Indian Journal of Helminthology, 4, 43–48Google Scholar
  14. Kulkarni T. 1969. On a new species Ancylodiscoides Yamaguti, 1937 from the fish Wallagonia attu (Bloch) in Hyderabad, A.P. Zoologische Anzeiger, 182, 461–465Google Scholar
  15. Lim L.H.S. 1996. Thaparocleidus Jain, 1952, the senior synonym of Silurodiscoides Gussev, 1976 (Monogenea: Ancylodiscoidinae). Systematic Parasitology, 35, 207–215CrossRefGoogle Scholar
  16. Lim L.H.S. 1998. Diversity of monogeneans in Southeast Asia. International Journal of Parasitology, 28, 1495–1515CrossRefGoogle Scholar
  17. Lim L.H.S., Lerssutthichawal T. 1996. Monogeneans from Wallago attu (Bloch and Schneider, 1802) of Thailand. Raffles Bulletin of Zoology, 44, 287–300Google Scholar
  18. Lim L.H.S., Timofeeva T.A., Gibson D.I. 2001. Dactylogyridean monogeneans of the siluriform fishes of the Old World. Systematic Parasitology, 50, 159–197CrossRefGoogle Scholar
  19. Littlewood D.T.J., Olson P.D. 2001. Small subunit rDNA and the phylum Platyhelminthes: signal, noise, conflict and compromise. In: (Eds Littlewood, D.T.J., Bray, R.A.) Interrelationships of the Platyhelminthes. Taylor and Francis, London, U.K., pp. 262–278Google Scholar
  20. Mendoza-Palmero C.A., Blasco-Costa I., Scholz T. 2015. Molecular phylogeny of Neotropical monogeneans (Platyhelminthes: Monogenea) from catfishes (Siluriformes). Parasites & Vectors, 8, 164CrossRefGoogle Scholar
  21. Milne I., Lindner D., Bayer M., Husmeier D., McGuire G., Marshall D.F., Wright F. 2009. TOPALi v2: a rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multi-core desktops. Bioinformatics, 25, 126–127CrossRefGoogle Scholar
  22. Pandey K.C., Agarwal A. 1989. On a new monogenetic trematoda from Wallago attu (Bl.). Indian Journal of Helminthology (N.S.), 5, 51–53Google Scholar
  23. Pandey K.C., Agarwal N. 2008. An encyclopaedia of Indian monogenoidea. Vitasta Publishing Pvt. Ltd., New Delhi, India, pp. 522Google Scholar
  24. Pandey K.C., Mehta T. 1986. Studies on some new monogenetic trematodes of Wallago attu (Bloch) at Meerut, Uttar Pradesh, India. In: Proceedings of the Indian National Convocation for Young Scientists, 114–132Google Scholar
  25. Posada D., Crandall K.A. 1998. Modeltest: Testing the model of DNA substitution. Bioinformatics, 14, 817–818CrossRefGoogle Scholar
  26. Šimková A., Serbielle C., Pariselle A., Vanhove M.P.M., Morand S. 2013. Speciation in Thaparocleidus (Monogenea: Dactylogyridae) parasitizing Asian pangasiid catfishes. BioMed Research International, 353956. DOI: 10.1155/2013/353956Google Scholar
  27. Singh H.S., Sharma B. 1992. Wallagotrema indicus n. sp., a new monogenean from the freshwater shark Wallago attu (Bloch and Schn). Functional Development and Morphology, 2, 85–88Google Scholar
  28. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular evolution genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729CrossRefGoogle Scholar
  29. Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680CrossRefGoogle Scholar
  30. Tripathi Y.R. 1959. Monogenetic trematodes from fishes of India. Indian Journal of Helminthology, 9, 1–149Google Scholar
  31. Van de Peer Y., De Rijk P., Wuyts J., Winkelmans T., De Wachter R. 2000. The European Small Subunit Ribosomal RNA database. Nucleic Acids Research, 28, 175–176.CrossRefGoogle Scholar
  32. Verma C., Chaudhary A., Singh H.S. 2015. Morphological redescription and first molecular analyses of two species of Mizelleus (Monogenea, Dactylogyridae) infecting the Indian Gangetic shark, Wallago attu. Journal of Helminthology 16, 1–11. DOI: 10.1017/S0022149X15000814Google Scholar
  33. Verma C., Chaudhary A., Singh H.S. 2016. Thaparocleidus gangus sp. nov. (Monogenea: Dactylogyridae) from gill filaments of Wallago attu Bloch and Schn., 1801, India. Turkish Journal of Zoology, 40: 758–764. DOI:10.3906/zoo-1507-31CrossRefGoogle Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2017

Authors and Affiliations

  • Chandni Verma
    • 1
    Email author
  • Anshu Chaudhary
    • 1
  • Hridaya Shanker Singh
    • 1
  1. 1.Molecular Taxonomy Laboratory, Department of ZoologyChaudhary Charan Singh UniversityMeerutIndia

Personalised recommendations