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Acta Parasitologica

, Volume 62, Issue 3, pp 625–629 | Cite as

Leishmania infantum is present in vaginal secretions of naturally infected bitches at lower levels in oestrogenized bitches than in non-oestrogenized bitches

  • Alinne Gonçalves Magro
  • Viviane Pedersoli Assis
  • Lucélia Coimbra Silva
  • Wagner Luiz Tafuri
  • Soraia Oliveira Silva
  • Maria Norma Melo
  • Guilherme Ribeiro ValleEmail author
Article

Abstract

Naturally Leishmania infantum infected bitches were divided into oestrogenized (n = 11) and non-oestrogenized (n = 6) groups. Vaginal secretions were collected for polymerase chain reaction (PCR), and vulval, vaginal and uterine tissues were collected for the immunohistochemical (IHQ) identification of L. infantum. Parasite DNA was identified in vaginal secretions of non-oestrogenized (41.8%) and oestrogenized (18.2%) bitches (P<0.05; Fisher’s Exact test). IHQ was positive in vulvar dermis (23.5%) and vaginal mucosa (17.7%) but negative in endometrium of all bitches. Poor association between positive vaginal secretion PCR and tissue IHQ (Kappa index) were observed. The results showed that genital secretions are a potential source for dog contamination.

Keywords

Leishmania infantum bitch genital secretions 

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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2017

Authors and Affiliations

  • Alinne Gonçalves Magro
    • 1
  • Viviane Pedersoli Assis
    • 1
  • Lucélia Coimbra Silva
    • 1
  • Wagner Luiz Tafuri
    • 3
  • Soraia Oliveira Silva
    • 3
  • Maria Norma Melo
    • 3
  • Guilherme Ribeiro Valle
    • 1
    • 2
    Email author
  1. 1.Departamento de Medicina Veterinária da Pontifícia Universidade Católica de Minas GeraisBetimBrazil
  2. 2.Programa de Pós-graduação em Biologia de Vertebrados da Pontifícia Universidade Católica de Minas GeraisBairro Coração EucarísticoBelo HorizonteBrazil
  3. 3.Instituto de Ciências Biológicas da Universidade Federal de Minas GeraisBelo HorizonteBrazil

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