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Acta Parasitologica

, Volume 62, Issue 3, pp 557–564 | Cite as

The Prevalence of Cyclospora cayetanensis and Cryptosporidium spp. in Turkish patients infected with HIV-1

  • Hayriye Kirkoyun Uysal
  • Gokhan Tolga Adas
  • Kevser Atalik
  • Semih Altiparmak
  • Ozer Akgul
  • Suat Saribas
  • Mert Gurcan
  • Pelin Yuksel
  • Taner Yildirmak
  • Bekir KocazeybekEmail author
  • Tevhide Ziver
  • Yasar Ali Oner
Article

Abstract

Opportunistic infections such as cryptosporidiosis and cyclosporiasis are commonly encountered in patients with acquired immunodeficiency syndrome (AIDS).We investigated the existence of opportunistic protozoans that significantly affect the quality of life in HIV-1 infected patients using conventional and molecular methods. The study group comprised 115 HIV-1 positive patients. In the identification of Cyclospora cayetanensis and Cryptosporidium, the formol-ether precipitation method was used and smears were evaluated in optical microscope by staining modified Ziehl-Neelsen (ZN). The primers and probes used for PCR were Heat shock protein 70 for C. cayetanensis and the oocysts wall protein for Cryptosporidium spp.. Cyclospora and Cryptosporidium spp. oocysts were detected in one and two patients, respectively, by staining, whereas we detected C. cayetanensis in three patients out of 115 (2.6%) by PCR, and Cryptosporidium spp. in a further three patients (2.6%). C. cayetensis was detected in patients with CD4 counts of 64 cells/μm, 182 cells/μm and 287 cells/μm, respectively. Cryptosporidium spp. was detected in patients with CD4 counts of 176 cells/μm, 241 cells/μm and 669 cells/μm. As conclusion, PCR method is faster and more sensitive than microscopic methods and to screen intestinal pathogens routinely in patients infected with HIV should not be neglected in developing countries like Turkey.

Keywords

Cyclospora cayetanensis Cryptosporidium spp HIV-1 

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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2017

Authors and Affiliations

  • Hayriye Kirkoyun Uysal
    • 1
  • Gokhan Tolga Adas
    • 2
  • Kevser Atalik
    • 1
  • Semih Altiparmak
    • 1
  • Ozer Akgul
    • 3
  • Suat Saribas
    • 4
  • Mert Gurcan
    • 5
  • Pelin Yuksel
    • 4
  • Taner Yildirmak
    • 6
  • Bekir Kocazeybek
    • 4
    Email author
  • Tevhide Ziver
    • 7
  • Yasar Ali Oner
    • 1
  1. 1.Istanbul University İstanbul Medical Faculty Department of Medical MicrobiologyIstanbulTurkey
  2. 2.Okmeydanı Training and Research Hospital Department of General SurgeryIstanbulTurkey
  3. 3.Üsküdar University Vocational School of Health ServicesIstanbulTurkey
  4. 4.Istanbul University Cerrahpasa Medical Faculty Department of Medical MicrobiologyIstanbulTurkey
  5. 5.Medical Faculty Studentİstanbul University Cerrahpasa Medical FacultyIstanbulTurkey
  6. 6.Department of Microbiology and Clinical MicrobiologyOkmeydanı Training and Research HospitalIstanbulTurkey
  7. 7.Health Sciences FacultyEast Mediterranean UniversityGazimagusaNorth Cyprus

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