Acta Parasitologica

, Volume 61, Issue 4, pp 849–854 | Cite as

The PCR and nested PCR detection of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Dermacentor reticulatus F. collected in a new location in Poland (Trzciel, Western Poland)

  • Patrycja OpalińskaEmail author
  • Anna Wierzbicka
  • Marek Asman


The study was performed in the Trzciel Forest Districts which is located in the west part of Poland. The Scots pine is the main tree species creating forest landscape there. Dermacentor reticulatus, usually found in wet, boggy and damp habitat, in this case was discovered in fresh mixed coniferous forest and fresh coniferous forest. In Central Europe the Dermacentor reticulatus is after Ixodes ricinus the second most important vector for tick-borne diseases in Europe. The ticks were collected by flagging from lower vegetation during the autumn peak of their activity. All Dermacentor reticulatus were checked for presence of tickborne pathogens using PCR and nested PCR. In total 125 Dermacentor reticulatus ticks were collected. Among the pathogens examined, only Babesia microti has been found in 4% of them whereas Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum have not been found. In this research the presence of Babesia. microti in Dermacentor reticulatus has been proved. It should be stressed that it is a new focus for the Dermacentor reticulatus tick in Western Poland.


Dermacentor reticulatus Babesia microti Borrelia burgdorferi sensu lato Anaplasma phagocytophilum Trzciel Forest District new focus of the occurrence 


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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2016

Authors and Affiliations

  • Patrycja Opalińska
    • 1
    Email author
  • Anna Wierzbicka
    • 1
  • Marek Asman
    • 2
  1. 1.Department of Game Management and Forest ProtectionPoznań University of Life SciencesPoznańPoland
  2. 2.Department of ParasitologyMedical University of SilesiaSosnowiecPoland

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