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Acta Parasitologica

, Volume 61, Issue 4, pp 790–795 | Cite as

A genetic structure of novel population of Fascioloides magna from Poland, Podkarpackie Province, indicates an expanding second European natural focus of fascioloidosis

  • Ľudmila Juhásová
  • Eva Bazsalovicsová
  • Ivica Králová-HromadováEmail author
  • Jacek Karamon
Article

Abstract

The veterinary important parasite of ruminants, giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), isolated from liver of farmed fallow deer (Dama dama) from Podkarpackie Province (southeastern Poland) was genotypized by mitochondrial cytochrome c oxidase (cox1) and nicotinamide dehydrogenase (nad1) markers. The data on this newly emerged population were compared with mitochondrial haplotypes of recently detected Polish population of F. magna from Lower Silesian Wilderness (southwestern Poland) and with European populations of the parasite from all three natural foci; northern Italy, Czech Republic and the Danube floodplain forests. The flukes from Podkarpackie Province were found to be genetically identical with flukes from Czech Republic and Lower Silesian Wilderness in Poland. It is evident that central and southwestern Czech Republic, recognized as one of the endemic area of F. magna in Europe, has been enlarging and parasite has been invading several novel localities in Poland.

Note: Nucleotide sequence data reported in this paper are available in the GenBankTM, EMBL and DDBJ databases under the accession numbers KX279954 - 5

Keywords

Giant liver fluke fallow deer mitochondrial DNA coxnadnatural focus 

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References

  1. Apostolo C. 1996. The naturalistic aspects: flora, fauna and the environment. In: (Eds M. Lupo, M. Paglieri, C. Apostolo, E. Vaccarino and M. Debernardi) La Mandria Storia e natura del Parco. Edizioni Eda, Torino, 67–98Google Scholar
  2. Bazsalovicsová E., Králová-Hromadová I., Spakulová M., Reblánová M., Oberhauserová K. 2010. Determination of ribosomal internal transcribed spacer 2 (ITS2) interspecific markers in Fasciola hepatica, Fascioloides magna, Dicrocoelium dendriticum and Paramphistomum cervi (Trematoda), parasites of wild and domestic ruminants. Helminthologia, 47, 76–82. DOI: 10.2478/s11687-010-0011-1CrossRefGoogle Scholar
  3. Bazsalovicsová E., Králová-Hromadová I., Štefka J., Minárik G., Bokorová S., Pybus M. 2015. Genetic interrelationships of North American populations of giant liver fluke Fascioloides magna. Parasites & Vectors, 8, 288. DOI: 10.1186/s13071015-0895-1CrossRefGoogle Scholar
  4. Demiaszkiewicz A.W., Kuligowska I., Pyziel A.M., Lachowicz J., Kowalczyk R. 2015. Extension of occurrence area of the American fluke Fascioloides magna in south-western Poland. Annals of Parasitology, 61, 93–96PubMedGoogle Scholar
  5. Demiaszkiewicz A.W., Pyziel A.M., Kuligowska I., Lachowicz J. 2016. Fascioloides magna pasozytem jeleni w Borach Dolnoslqskich. Medycyna Weterynaryjna, 72, 110–112. (In Polish)Google Scholar
  6. Foreyt W.J., Todd A.C. 1976. The development of the large American liver fluke, Fascioloides magna, in white-tailed deer, cattle, and sheep. Journal of Parasitology, 62, 26–32. DOI: 10.2307/3279036CrossRefGoogle Scholar
  7. Karamon J., Larska M., Jasik A., Sell B. 2015. First report of the giant liver fluke (Fascioloides magna) infection in farmed fallow deer (Dama dama) in Poland — pathomorphological changes and molecular identification. Bulletin of the Veterinary Institute in Pulawy, 59, 339–344. DOI:10.1515/bvip2015-0050CrossRefGoogle Scholar
  8. Kašny M., Beran L., Siegelová V., Siegel T., Leontovyc R., Beránková K., Pankrác J., Košt’áková M., Horák P. 2012. Geographical distribution of the giant liver fluke (Fascioloides magna) in the Czech Republic and potential risk of its further spread. Veterinarni Medicina, 57, 101–109CrossRefGoogle Scholar
  9. Králová-Hromadová I., Spakulová M., Horácková E., Turceková L., Novobilsky A., Beck R., Koudela B., Marinculic A., Rajsky D., Pybus M. 2008. Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica. Journal of Parasitology, 94, 58–67. DOI:10.1645/GE-1324.1CrossRefGoogle Scholar
  10. Králová-Hromadová I., Bazsalovicsová E., Štefka J., Spakulová M., Vávrová S., Szemes T., Tkach V., Pybus M. 2011. Multiple origins of European populations of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), a liver parasite of ruminants. International Journal for Parasitology, 41, 373–383. DOI: 10.1016/j.ijpara.2010.10.010CrossRefGoogle Scholar
  11. Králová-Hromadová I., Bazsalovicsová E., Demiaszkiewicz A.W. 2015. Molecular characterization of Fascioloides magna (Trematoda: Fasciolidae) from south-western Poland based on mitochondrial markers. Acta Parasitologica, 60, 544–547. DOI: 10.1515/ap-2015-0077CrossRefGoogle Scholar
  12. Králová-Hromadová I., Juhásová E., Bazsalovicsová E. (Eds). 2016. Giant liver fluke, Fascioloides magna: past, present and future research. Springer International Publishing, Switzerland Springer Briefs in Animal Sciences, pp. 106 DOI: 10.1007/978-3-319-29508-4Google Scholar
  13. Novobilsky A., Horácková E., Hirtová L., Modry D., Koudela B. 2007. The giant liver fluke Fascioloides magna (Bassi 1875) in cervids in the Czech Republic and potential of its spreading to Germany. Parasitology Research, 100, 549–553. DOI: 10.1007/s00436-006-0299-4CrossRefGoogle Scholar
  14. Plötz C., Rehbein S., Bamler H., Reindl H., Pfister K., Scheuerle M.C. 2015. Fascioloides magna — epizootiology in a deer farm in Germany. Berliner und Münchener tierärztliche Wochenschrift, 128, 177–182. DOI: 10.2376/0005-9366-128-177PubMedGoogle Scholar
  15. Pybus M.J. 2001. Liver flukes. In: (Eds W.M. Samuel, M.J. Pybus and A.A. Kocan) Parasitic diseases of wild mammals. Edn. 2. Iowa State University Press, Ames, 121–149CrossRefGoogle Scholar
  16. Pyziel A.M., Demiaszkiewicz A.W., Kuligowska I. 2014. Molecular identification of Fascioloides magna (Bassi, 1875) from red deer from south-western Poland (Lower Silesian Wilderness) on the basis of internal transcribed spacer 2 (ITS-2). Polish Journal of Veterinary Sciences, 17, 523–525. DOI: 10.2478/pjvs-2014-0077CrossRefGoogle Scholar
  17. Rehbein S., Hamel D., Reindl H., Visser M., Pfister K. 2012. Fascioloides magna and Ashworthius sidemi — two new parasites in wild ungulates in Germany. In: Program and Abstracts of the XI European Multicolloquium of Parasitology (EMOP XI), 25–29 July, 2012, Cluj-Napoca, Romania, 565Google Scholar
  18. Salomon S. (1932) Fascioloides magna bei deutschem Rotwild. Berliner und Münchener tierärztliche Wochenschrift, 48, 627–628. (In German)Google Scholar
  19. Slusarski W. 1955. Studia nad europejskimi przedstawicielami przywry Fasciola magna (Bassi, 1875) Stiles, 1894. Acta Parasitologica Polonica, 3, 1–59. (In Polish)Google Scholar
  20. Swales W.E. 1935. The life cycle of Fascioloides magna (Bassi, 1875), the large liver fluke of ruminants, in Canada. Canadian Journal of Research, 12, 177–215. DOI: 10.1139/cjr35 015CrossRefGoogle Scholar
  21. Ullrich K. 1930. Über das Vorkommen von seltenen oder wenig bekannten Parasiten der Säugetiere und Vögel in Böhmen und Mähren. Prag Arch Tiermed, 10, 19–43. (In German)Google Scholar
  22. Werle E., Schneider C., Renner M., Volker M., Fiehn W. 1994. Convenient single-step, one tube purification of PCR products for direct sequencing. Nucleic Acids Research, 22, 4354–4355CrossRefGoogle Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2016

Authors and Affiliations

  • Ľudmila Juhásová
    • 1
  • Eva Bazsalovicsová
    • 1
  • Ivica Králová-Hromadová
    • 1
    Email author
  • Jacek Karamon
    • 2
  1. 1.Institute of ParasitologySlovak Academy of SciencesKošiceSlovakia
  2. 2.Department of Parasitology and Invasive DiseasesNational Veterinary Research InstitutePulawyPoland

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