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Acta Parasitologica

, Volume 61, Issue 4, pp 776–783 | Cite as

Fasciola hepática infection in humans: overcoming problems for the diagnosis

  • Silvana Carnevale
  • Jorge Bruno Malandrini
  • María Laura Pantano
  • Mirna Sawicki
  • Claudia Cecilia Soria
  • Lein Hung Kuo
  • Laura Kamenetzky
  • Osvaldo Germán Astudillo
  • Jorge Néstor VelásquezEmail author
Article

Abstract

Fascioliasis is a zoonosis actually considered as a foodborne trematode disease priority by the World Health Organization. Our study presents three cases of F. hepatica infection diagnosed by direct, indirect and/or imaging diagnostic techniques, showing the need of the combined use of them. In order to overcome some difficulties of the presently available methods we show for the first time the application of molecular tools to improve human fascioliasis diagnosis by employing a PCR protocol based on a repetitive element as target sequence. In conclusion, diagnosis of human fascioliasis has to be carried out by the combination of diagnostic techniques that allow the detection of infection in different disease phases, different epidemiological situations and known/new transmission patterns in the actual scenario.

Keywords

Fascioliasis polymerase chain reaction enzyme-linked immunosorbent assay 

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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2016

Authors and Affiliations

  • Silvana Carnevale
    • 1
    • 2
  • Jorge Bruno Malandrini
    • 3
  • María Laura Pantano
    • 1
  • Mirna Sawicki
    • 4
  • Claudia Cecilia Soria
    • 3
  • Lein Hung Kuo
    • 5
  • Laura Kamenetzky
    • 2
    • 6
  • Osvaldo Germán Astudillo
    • 1
  • Jorge Néstor Velásquez
    • 4
    Email author
  1. 1.Instituto Nacional de Enfermedades Infecciosas — ANLIS “Dr. Carlos G. Malbrán”Ciudad de Buenos AiresArgentina
  2. 2.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Argentina
  3. 3.Facultad de Ciencias de la SaludUniversidad Nacional de CatamarcaSan Fernando del Valle de Catamarca, CatamarcaArgentina
  4. 4.Hospital Municipal de Infecciosas “Dr. Francisco Javier Muñiz”Ciudad de Buenos AiresArgentina
  5. 5.Hospital Samic OberáPincel Federación s/nOberá, MisionesArgentina
  6. 6.Instituto de Microbiología y Parasitología Médica (IMPaM), UBA-CONICETCiudad de Buenos AiresArgentina

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