Acta Parasitologica

, Volume 61, Issue 4, pp 756–761 | Cite as

Detection of Theileria lestoquardi cross infection in cattle with clinical theileriosis in Iran

  • Seyedeh Missagh JalaliEmail author
  • Abbas Jolodar
  • Aria Rasooli
  • Ameneh Darabifard


Theileriosis caused by Theileria lestoquardi (malignant ovine theileriosis) in sheep and Theileria annulata (tropical theileriosis) in cattle is an important hemoprotozoal tick-borne disease in Iran. Due to major biologic and phylogenic similarities of these two species, this study was carried out to investigate the occurrence of natural infections with T.lestoquardi and T.annulata in cattle with clinical theileriosis in Ahvaz, southwest Iran. Fifty one cattle were selected based on clinical signs of theileriosis and confirmation by microscopic examination of blood smears. Blood samples were collected from each animal and hematologic and microscopic examinations were performed. Theileria piroplasmic forms were detected in all affected cattle. Pale mucous membranes (43.14%), icterus (11.76%) and fever (70.6%) were also observed. PCR-RFLP analysis revealed T annulata infection in all tested cattle while coinfections with T lestoquardi were found in two samples (3.92%). All sampled cattle including the two with mixed species Theileria infection were anemic. This is the first report of Theileria species cross infections in cattle with clinical theileriosis in Iran. It can be concluded that cattle can be infected with both pathogenic Theileria species, T lestoquardi and T annulata which can be an important issue in the epidemiology and spread of ovine malignant theileriosis.


Theileria lestoquardi Theileria annulata cross infection cattle Iran 


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  1. Ahmed B.M., El Hussein A.M., El Ghali A., Salih D.A. 2003. Some Studies on the epidemiology of ovine theileriosis in River Nile State, Northern Sudan. Journal of Animal and Veterinary Advances, 2, 681–685Google Scholar
  2. Asri Rezaei S., Dalir-Naghadeh B. 2006. Evaluation of antioxidant status and oxidative stress in cattle naturally infected with Theileria annulata. Veterinary Parasitology, 142, 179–186. D0I:10.1016/j.vetpar.2006.05.033CrossRefGoogle Scholar
  3. Dolan T.T. 1989. Theileriosis: a comprehensive review. Revue Scientifique et Technique (International Office of Epizootics), 8, 11–36Google Scholar
  4. Hashemi-Fesharki R. 1988. Control of Theileria annulata in Iran. Parasitology Today, 4, 36–40. D0I:10.1016/0169-4758(88)90062-2CrossRefGoogle Scholar
  5. Hashemi-Fesharki R. 1997. Tick-borne diseases of sheep and goats and their related vectors in Iran. Parassitologia, 39, 115–117PubMedGoogle Scholar
  6. Heidarpour Bami M., Haddadzadeh H.R., Kazemi B., Khazraiinia R, Bandehpour M., Aktas M. 2009. Molecular identification of ovine Theileria species by a new PCR-RFLP method. Veterinary Parasitology, 161, 171–177. D0I:10.1016/j.vetpar.2009.01.035CrossRefGoogle Scholar
  7. Hooshmand-Rad P., Hawa N.J. 1973. Transmission of Theileria hirci in sheep by 246 Hyalomma anatolicum anatolicum. Tropical Animal Health and Production, 5, 103–109'CrossRefGoogle Scholar
  8. Jalali S.M., Khaki Z., Kazemi B., Rahbari S., Shayan P., Bandehpour M., Yasini S.P. 2014. Molecular Detection and Identification of Theileria Species by PCR-RFLP Method in Sheep from Ahvaz, Southern Iran. Iranian Journal of Parasitology, 9, 99–106PubMedPubMedCentralGoogle Scholar
  9. Kirvar E., Ilhan T., Katzer F., Wilkie G., Hooshmand-Rad P., Brown D. 1998. Detection of Theileria lestoquardi (hirci) in ticks, sheep, and goats using the polymerase chain reaction. Annals of the New York Academy of Sciences, 849, 52–62. DOI: 10.1111/j.1749-6632.1998.tb11033.xCrossRefGoogle Scholar
  10. Leemans I., Hooshmand-Rad P., Brown C.G.D., Wilkie G., Uggla A. 1998. In Vitro infectivity and In vivo cross-protectivity of Theileria lestoquardi and T annulata in sheep and cattle. Annals of the New York Academy of Sciences, 849, 408–411. DOI: 10.1111/j.1749-6632.1998.tb11083.xCrossRefGoogle Scholar
  11. Leemans I., Brown D., Fossum C., Hooshmand-Rad P., Kirvar E., Wilkie G., Uggla A. 1999a. Infectivity and cross-immunity studies of Theileria lestoquardi and Theileria annulata in sheep and cattle II. In vitro studies. Veterinary Parasitology, 82, 193–204. DOI:10.1016/S0304-4017(99)00014-XCrossRefGoogle Scholar
  12. Leemans I., Brown D., Hooshmand-Rad P., Kirvar E., Uggla A. 1999b. Infectivity and cross-immunity studies of Theileria lestoquardi and Theileria annulata in sheep and cattle I. In vivo responses. Veterinary Parasitology, 82, 179–192. D0I:10.1016/S0304-4017(99)00013-8CrossRefGoogle Scholar
  13. Leemans I., Hooshmand-Rad P., Uggla A. 1997. The indirect fluorescent antibody test based on schizont antigen for the study of the sheep parasite Theileria lestoquardi. Veterinary Parasitology, 69, 9–18. D0I:10.1016/S0304-4017(96)01098-9CrossRefGoogle Scholar
  14. Li Y., Liu Z., Yang J., Chen Z., Guan G., Niu Q., Zhang X., Luo J., Yin H. 2014. Infection of small ruminants and their red blood cells with Theileria annulata schizonts. Experimental Parasitology, 137, 21–24. D0I:10.1016/j.exppara.2013.11.010CrossRefGoogle Scholar
  15. Mans B.J., Pienaar R., Latif A.A. 2015. A review of Theileria diagnostics and epidemiology. International Journal for Parasitology: Parasites and Wildlife, 4, 104–118PubMedGoogle Scholar
  16. Nabian S., Rahbari S., Changizi A., Shayan P. 2009. The distribution of Hyalomma spp. ticks from domestic ruminants in Iran. Medical and Veterinary Entomology, 23, 281–283. DOI: 10.1111/j.1365-2915.2009.00804.xCrossRefGoogle Scholar
  17. Omer O.H., El-Malik K.H., Mahmoud O.M., Haroun E.M., Hawas A., Sweeney D., Magzoub M. 2002. Haematological profiles in pure bred cattle naturally infected with Theileria annulata in Saudi Arabia. Veterinary Parasitology, 107, 161–168. DOI:10.1016/S0304-4017(02)00094-8CrossRefGoogle Scholar
  18. Rahbari S., Nabian S., Shayan P. 2007. Primary report on distribution of tick fauna in Iran. Parasitology Research, 101, 175–177CrossRefGoogle Scholar
  19. Razmi G., Yaghfoori S. 2013. Molecular surveillance of Theileria ovis, Theileria lestoquardi and Theileria annulata infection in sheep and ixodid ticks in Iran. Onderstepoort Journal of Veterinary Research, 80, 635–640CrossRefGoogle Scholar
  20. Robinson P.M. 1982. Theileria annulata and its transmission a review. Tropical Animal Health and Production, 14, 3–12CrossRefGoogle Scholar
  21. Safapoor Dehkordi F., Parsaei P., Saberian S., Moshkelani S., Hajshafiei P., Hoseini S.R., Babaei M., Ghorbani M.N. 2012. Prevalence study of Theileria annulata by comparison of four diagnostic techniques in southwest Iran. Bulgarian Journal of Veterinary Medicine, 15, 123–130Google Scholar
  22. Schnittger L., Yin H., Jianxun L., Lugwing W., Shayan P., Rahbari S., Voss-Holtmann A., Ahmed J.S. 2000. Ribosomal smallsubunit RNA gene-sequence analysis of Theileria lestoquardi and a Theileria species highly pathogenic for small ruminants in China. Parasitology Research, 86, 352–358CrossRefGoogle Scholar
  23. Sofizadeh A., Telmadarraiy Z., Rahnama A., Gorganli-Davaji A., Hosseini-Chegeni A. 2014. Hard tick species of livestock and their bioecology in Golestan province, north of Iran. Journal of Arthropod Borne Diseases, 8, 108–116PubMedGoogle Scholar
  24. Taha K.M., Salih D.A., Ali A.M., Omer R.A., El Hussein A.M. 2013. Naturally occurring infections of cattle with Theileria lestoquardi and sheep with Theileria annulata in the Sudan. Veterinary Parasitology, 191, 143–145. DOI: 10.1016/j.vetpar.2012.08.003CrossRefGoogle Scholar
  25. Tavassoli M., Tabatabaei M., Esmaeil Nejad B., Hasani Tabatabaei M., Najafabadi A., Pourseyed S.H. 2011. Detection of Theileria annulata by the PCR-RFLP in ticks (Acari,Ixodidae) collected. ActaParasitologica, 56, 8–13. DOI: 10.2478/s11686-011-0001-6Google Scholar
  26. Zaeemi M., Haddadzadeh H., Khazraiinia P., Kazemi B., Bandehpou M. 2011. Identification of different Theileria species (Theileria lestoquardi, Theileria ovis, and Theileria annulata) in naturally infected sheep using nested PCR-RFLP. Parasitology Research, 108, 837–843CrossRefGoogle Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2016

Authors and Affiliations

  • Seyedeh Missagh Jalali
    • 1
    Email author
  • Abbas Jolodar
    • 2
  • Aria Rasooli
    • 1
    • 3
  • Ameneh Darabifard
    • 4
  1. 1.Department of Clinical Sciences, Faculty of Veterinary MedicineShahid Chamran University of AhvazAhvazIran
  2. 2.Department of Basic Sciences, Faculty of Veterinary MedicineShahid Chamran University of AhvazAhvazIran
  3. 3.Department of Animal Health Management, School of Veterinary MedicineShiraz UniversityShirazIran
  4. 4.Graduate student, Faculty of Veterinary MedicineShahid Chamran University of AhvazAhvazIran

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