Advertisement

Acta Parasitologica

, Volume 61, Issue 4, pp 694–696 | Cite as

Comparison of indirect fluorescent antibody test and the modified agglutination test for the detection of Toxoplasma gondii antibodies in stray dogs from Southern Brazil

  • Jonatas Campos de Almeida
  • Michelle Salmon Frehse
  • Italmar Teodorico Navarro
  • João Luis Garcia
  • Alexander Welker Biondo
  • Roberta Lemos FreireEmail author
Article
  • 1 Downloads

Abstract

The aim of the present study was to determine the prevalence of antibodies to Toxoplasma gondii by two serological techniques in sera of 364 stray dogs from Brazil by immunofluorescence antibody test (IFAT, cut off point 1:16) and to the modified agglutination test (MAT, cut-off points 1:25 and 1:50). A total of 175/364 (48.07%) sera were positive by IFAT, and 108/364 (29.67%) and 85/364 (23.35%) were positive by MAT with cutoff points 1:25 and 1:50, respectively were positive by MAT. Cohen’s Kappa Coefficient between IFAT and MAT was 0.81 (excellent) and 0.66 (substantial) with cutoff points 1:25 and 1:50, respectively. Using IFAT as gold standard, MAT sensitivity and specificity were 78% and 99% for 1:25 and 61% and 99% for 1:50, respectively. The results document of the usefulness of MAT for serological diagnosis because it does not require species-specific conjugate.

Keywords

Toxoplasma gondii stray dog MAT IFAT sentine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Camargo M.E. 1974. Introduction to immunofluorescence technique. Revista Brasileira de Patologia Clínica, 10, 143–171Google Scholar
  2. Cohen J. 1960. A coefficient of agreement for nominal scales. Educational and Psychological Measurement, 20, 37–46CrossRefGoogle Scholar
  3. Desmonts G., Remington J. 1980. Direct agglutination test for diagnosis of Toxoplasma infection: method for increasing sensitivity and specificity. Journal of Clinical Microbiology, 1980, 11, 562–568Google Scholar
  4. Dubey J.P., Desmonts G. 1987. Serological responses of equids fed Toxoplasma gondii oocysts. Equine Veterinary Journal. 19, 337–339CrossRefGoogle Scholar
  5. Dubey J.P. (Ed.). 2010. Toxoplasmosis of Animals and Humans. 2.ed. New York, CRC Press Taylor & Francis Group. pp. 313Google Scholar
  6. Dubey J.P., Lago E.G., Gennari S.M., Su C., Jones J.L. 2012. Toxoplasmosis in humans and animals in Brazil: high prevalence, high burden of disease, and epidemiology. Parasitology. 139, 1375–1424CrossRefGoogle Scholar
  7. Dubey J.P., Laurin E., Kwowk O.C.H. 2016. Validation of the modified agglutination test for detection of Toxoplasma gondii in free-range chickens by using cat and mouse bioassay. Parasitology. 143, 314–319CrossRefGoogle Scholar
  8. Frenkel J.K., Lindsay D.S., Parker B.B. 1995. Dogs as potential vectors of Toxoplasma gondii. The American Journal of Tropical Medicine and Hygiene, 53, 226CrossRefGoogle Scholar
  9. Gart J.J., Buck A.A. 1966. Comparison of a screening test and a reference test in epidemiologic studies. II. A probabilistic model for the comparison of diagnostic tests. American Journal of Epidemiology, 83, 593–602CrossRefGoogle Scholar
  10. Landis J.R., Koch G.G. 1977. The measurement of observer agreement for categorical data. Biometrics, 33, 159–174CrossRefGoogle Scholar
  11. Marques J.M., Isbrecht F.B., Lucas T.M., Guerra I.M.P., Dalmolin A., Silva R.C., Langoni H., Silva A.V. 2009. Anti-Toxoplasma gondii antibody detection in animals from farms of a rural community of south of Mato Grosso do Sul, Brasil. Semina: Ciências Agrárias, 30, 889–898. DOI: http://dx.doi.org/10.5433/1679-0359.2009v30n4p889 (In Portuguese)Google Scholar
  12. Schares G., Pantchev N., Barutzi D., Heydorn A.O., Bauer C., Conraths F.J. 2005. Oocysts of Neospora caninum, Hammondia heydorni, Toxoplasma gondii and Hammondia hammondi in faeces collected from dogs in Germany. International Journal for Parasitology, 35, 1525–1537. DOI:10.1016/j.ijpara.2005.08.008CrossRefGoogle Scholar
  13. Souza S.L.P., Gennari S.M., Yai L.E.O., D’Auria S.R.N., Cardoso S.M.S., Guimaräes-Jünior J.S., Dubey J.P. 2003. Occurrence of Toxoplasma gondii antibodies in sera from dogs of the urban and rural areas from Brazil. Revista Brasileira de Parasitología Veterinária, 12, 1–3Google Scholar
  14. Thrusfield M.V. (Ed.). 2004. Veterinary Epidemiology. 2.ed. Säo Paulo, (Roca). pp. 556 (In Portuguese)Google Scholar
  15. Vidotto O. 1992. Toxoplasmosis: epidemiology and importance of the disease in animal health. Semina: Ciências Agrárias, 13, 69–75. DOI: http://dx.doi.org/10.5433/1679-0359.1992v13n1p69 (In Portuguese)Google Scholar

Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2016

Authors and Affiliations

  • Jonatas Campos de Almeida
    • 1
  • Michelle Salmon Frehse
    • 1
  • Italmar Teodorico Navarro
    • 2
  • João Luis Garcia
    • 2
  • Alexander Welker Biondo
    • 3
  • Roberta Lemos Freire
    • 2
    Email author
  1. 1.Laboratory of Zoonoses and Public HealthUniversidade Estadual de LondrinaBrazil
  2. 2.Department of Preventive Veterinary MedicineUniversidade Estadual de LondrinaBrazil
  3. 3.Department of Veterinary MedicineUniversidade Federal do ParanáBrazil

Personalised recommendations